Calcium Fluxes in Work-Related Muscle Disorder: Implications from a Rat Model

J. Hadrevi, M. F. Barbe, N. Ørtenblad, U. Frandsen, E. Boyle, S. Lazar, G. Sjøgaard, K. Søgaard*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Introduction: Ca2+ regulatory excitation-contraction coupling properties are key topics of interest in the development of work-related muscle myalgia and may constitute an underlying cause of muscle pain and loss of force generating capacity.

Method: A well-established rat model of high repetition high force (HRHF) work was used to investigate if such exposure leads to an increase in cytosolic Ca2+ concentration ([Ca2+]i) and changes in sarcoplasmic reticulum (SR) vesicle Ca2+ uptake and release rates.

Result: Six weeks exposure of rats to HRHF increased indicators of fatigue, pain behaviors, and [Ca2+]i, the latter implied by around 50-100% increases in pCam, as well as in the Ca2+ handling proteins RyR1 and Casq1 accompanied by an ∼10% increased SR Ca2+ uptake rate in extensor and flexor muscles compared to those of control rats. This demonstrated a work-related altered myocellular Ca2+ regulation, SR Ca2+ handling, and SR protein expression.

Discussion: These disturbances may mirror intracellular changes in early stages of human work-related myalgic muscle. Increased uptake of Ca2+ into the SR may reflect an early adaptation to avoid a sustained detrimental increase in [Ca2+]i similar to the previous findings of deteriorated Ca2+ regulation and impaired function in fatigued human muscle.

Original languageEnglish
Article number5040818
JournalBioMed Research International
Number of pages14
Publication statusPublished - 30. Sep 2019


  • Animals
  • Calcium/metabolism
  • Calcium-Binding Proteins/metabolism
  • Cytosol/metabolism
  • Disease Models, Animal
  • Excitation Contraction Coupling/physiology
  • Female
  • Mitochondrial Proteins/metabolism
  • Muscle Contraction/physiology
  • Muscle, Skeletal/metabolism
  • Muscular Diseases/metabolism
  • Myalgia/metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Ryanodine Receptor Calcium Release Channel/metabolism
  • Sarcoplasmic Reticulum/metabolism
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism


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