Abstract
CONTEXT: Regulation of IGF-I activity appears crucial in anorexia nervosa (AN) during adaptation to chronic starvation as well as during the regenerative processes on nutritional restoration. OBJECTIVE: The objective of this study was to examine the relationship between IGF-I bioactivity and IGF-binding capacity as expressed as formation of the binary complex of IGF-binding protein-1 (IGFBP-1) and IGF-I in patients with AN at different stages and with different subtypes of the disease. DESIGN: This was a longitudinal study. SETTING: The study took place at a clinical research center at a university hospital. STUDY PARTICIPANTS: We studied a total of 45 women with AN and 24 age-comparable healthy controls. MAIN OUTCOME MEASURES: IGF-I bioactivity was determined using an IGF-I receptor activation assay, and IGF-I/IGFBP-1 complex formation was determined by an assay that allows direct determination of the binary complex. RESULTS: IGF-I bioactivity was significantly decreased in serum from patients with AN. We found significant correlations between total, ultrafiltered free, and bioactive IGF-I. Despite increased IGFBP-1 concentrations, levels of IGF-I/IGFBP-1 binary complex were not significantly increased in AN. Oral contraceptives were associated with increased levels of IGF-I, IGFBP-1, and binary complex formation. Ghrelin levels were only significantly raised in those patients who had lost more than 5% of the body weight during the last 4 wk, whereas ghrelin levels in weight-stable as well as in weight-gaining patients did not significantly differ from the controls. CONCLUSIONS: Total IGF-I level is a suitable marker of IGF-I bioactivity in emaciated patients with AN irrespective of the clinical subtype and acute nutritional state.
Original language | English |
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Journal | The Journal of Clinical Endocrinology & Metabolism |
Volume | 92 |
Issue number | 6 |
Pages (from-to) | 2323-9 |
Number of pages | 6 |
ISSN | 0021-972X |
DOIs | |
Publication status | Published - 1. Jun 2007 |
Keywords
- Absorptiometry, Photon
- Adiponectin
- Adult
- Anorexia Nervosa
- Biological Markers
- Body Weight
- Cell Line
- Eating
- Female
- Ghrelin
- Homeostasis
- Humans
- Insulin Resistance
- Insulin-Like Growth Factor Binding Protein 1
- Insulin-Like Growth Factor I
- Kidney
- Longitudinal Studies
- Peptide Hormones
- Transfection