Abstract
We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.
Original language | English |
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Journal | Nature Methods |
Volume | 15 |
Issue number | 7 |
Pages (from-to) | 515-518 |
ISSN | 1548-7091 |
DOIs | |
Publication status | Published - 2018 |
Bibliographical note
Published: 21 May 2018Keywords
- Automation
- Carbohydrate Conformation
- Lipids/chemistry
- Mass Spectrometry/methods