Automated, parallel mass spectrometry imaging and structural identification of lipids

Shane R. Ellis, Martin R.L. Paine, Gert B. Eijkel, Josch K. Pauling, Peter Husen, Mark W. Jervelund, Martin Hermansson, Christer S. Ejsing*, Ron M.A. Heeren

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

Original languageEnglish
JournalNature Methods
Issue number7
Pages (from-to)515-518
Publication statusPublished - 2018

Bibliographical note

Published: 21 May 2018


  • Automation
  • Carbohydrate Conformation
  • Lipids/chemistry
  • Mass Spectrometry/methods


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