Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.

D Schledermann, B T Andersen, K Bisgaard, M Dohse, D Ejersbo, B Hoelund, P Horal, M Lindh, W Ryd

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16(INK4a) as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16(INK4a) interpretation.A total of 232 ThinPrep samples were stained for p16(INK4a), and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected.The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16(INK4a) and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16(INK4a) had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test.
Original languageEnglish
JournalDiagnostic Cytopathology
Volume36
Issue number7
Pages (from-to)453-9
Number of pages6
ISSN8755-1039
DOIs
Publication statusPublished - 1. Jul 2008

Keywords

  • Adult
  • Cervical Intraepithelial Neoplasia
  • Cervix Uteri
  • Cyclin-Dependent Kinase Inhibitor p16
  • DNA, Viral
  • Female
  • Histological Techniques
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Observer Variation
  • Papillomaviridae
  • Papillomavirus Infections
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Tumor Markers, Biological
  • Uterine Cervical Neoplasms
  • Vaginal Smears

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