Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.

D Schledermann; B T Andersen; K Bisgaard; M Dohse; D Ejersbo; B Hoelund; P Horal; M Lindh; W Ryd

doi:10.1002/dc.20822

Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.

D Schledermann, B T Andersen, K Bisgaard, M Dohse, D Ejersbo, B Hoelund, P Horal, M Lindh, W Ryd

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16(INK4a) as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16(INK4a) interpretation.A total of 232 ThinPrep samples were stained for p16(INK4a), and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected.The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16(INK4a) and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16(INK4a) had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test.

Original language	English
Journal	Diagnostic Cytopathology
Volume	36
Issue number	7
Pages (from-to)	453-9
Number of pages	6
ISSN	8755-1039
DOIs	https://doi.org/10.1002/dc.20822
Publication status	Published - 1. Jul 2008

Keywords

Adult
Cervical Intraepithelial Neoplasia
Cervix Uteri
Cyclin-Dependent Kinase Inhibitor p16
DNA, Viral
Female
Histological Techniques
Humans
Immunohistochemistry
Middle Aged
Observer Variation
Papillomaviridae
Papillomavirus Infections
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Tumor Markers, Biological
Uterine Cervical Neoplasms
Vaginal Smears

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10.1002/dc.20822

Cite this

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title = "Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.",

abstract = "The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16(INK4a) as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16(INK4a) interpretation.A total of 232 ThinPrep samples were stained for p16(INK4a), and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected.The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16(INK4a) and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16(INK4a) had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test.",

keywords = "Adult, Cervical Intraepithelial Neoplasia, Cervix Uteri, Cyclin-Dependent Kinase Inhibitor p16, DNA, Viral, Female, Histological Techniques, Humans, Immunohistochemistry, Middle Aged, Observer Variation, Papillomaviridae, Papillomavirus Infections, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Tumor Markers, Biological, Uterine Cervical Neoplasms, Vaginal Smears",

author = "D Schledermann and Andersen, {B T} and K Bisgaard and M Dohse and D Ejersbo and B Hoelund and P Horal and M Lindh and W Ryd",

year = "2008",

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doi = "10.1002/dc.20822",

language = "English",

volume = "36",

pages = "453--9",

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}

Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up. / Schledermann, D; Andersen, B T; Bisgaard, K; Dohse, M; Ejersbo, D; Hoelund, B; Horal, P; Lindh, M; Ryd, W.

In: Diagnostic Cytopathology, Vol. 36, No. 7, 01.07.2008, p. 453-9.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.

AU - Schledermann, D

AU - Andersen, B T

AU - Bisgaard, K

AU - Dohse, M

AU - Ejersbo, D

AU - Hoelund, B

AU - Horal, P

AU - Lindh, M

AU - Ryd, W

PY - 2008/7/1

Y1 - 2008/7/1

N2 - The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16(INK4a) as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16(INK4a) interpretation.A total of 232 ThinPrep samples were stained for p16(INK4a), and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected.The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16(INK4a) and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16(INK4a) had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test.

AB - The objectives of the study were to evaluate 1) the diagnostic sensitivity and specificity of p16(INK4a) as a marker for high-grade cervical lesions, 2) the results of a real-time polymerase chain reaction detecting high-risk human papillomavirus, and 3) the interobserver variability of the p16(INK4a) interpretation.A total of 232 ThinPrep samples were stained for p16(INK4a), and HPV-DNA PCR was performed on 107 specimens with inclusion of both benign and abnormal cytology. Histological follow-up information was collected.The diagnostic sensitivity of ASC+ with CIN2+ in histology as endpoint was 96% for p16(INK4a) and 100% for HR-HPV DNA PCR, and the diagnostic specificity was 41% and 27%, respectively. If p16(INK4a) had been used for triage of the ASC samples, then 18 patients (42%) could have been spared unnecessary follow-up procedures compared to six patients (21%) with the HR-HPV DNA test.

KW - Adult

KW - Cervical Intraepithelial Neoplasia

KW - Cervix Uteri

KW - Cyclin-Dependent Kinase Inhibitor p16

KW - DNA, Viral

KW - Female

KW - Histological Techniques

KW - Humans

KW - Immunohistochemistry

KW - Middle Aged

KW - Observer Variation

KW - Papillomaviridae

KW - Papillomavirus Infections

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Tumor Markers, Biological

KW - Uterine Cervical Neoplasms

KW - Vaginal Smears

U2 - 10.1002/dc.20822

DO - 10.1002/dc.20822

M3 - Journal article

C2 - 18528890

VL - 36

SP - 453

EP - 459

JO - Diagnostic Cytopathology

JF - Diagnostic Cytopathology

SN - 8755-1039

IS - 7

ER -

Are adjunctive markers useful in routine cervical cancer screening? Application of p16(INK4a) and HPV-PCR on ThinPrep samples with histological follow-up.

Abstract

Keywords

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