Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

Frank J. Hernandez, Neerja Kalra, Jesper Wengel, Birte Vester

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.
Original languageEnglish
JournalBioorganic & Medicinal Chemistry Letters
Volume19
Issue number23
Pages (from-to)6585-6587
Number of pages3
ISSN0960-894X
DOIs
Publication statusPublished - 2009

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Nucleotide Aptamers
Avidin
Monomers
Surface Plasmon Resonance
Surface plasmon resonance
Kinetics
Experiments
amino-LNA

Cite this

Hernandez, Frank J. ; Kalra, Neerja ; Wengel, Jesper ; Vester, Birte. / Aptamers as a model for functional evaluation of LNA and 2'-amino LNA. In: Bioorganic & Medicinal Chemistry Letters. 2009 ; Vol. 19, No. 23. pp. 6585-6587.
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Aptamers as a model for functional evaluation of LNA and 2'-amino LNA. / Hernandez, Frank J.; Kalra, Neerja; Wengel, Jesper; Vester, Birte.

In: Bioorganic & Medicinal Chemistry Letters, Vol. 19, No. 23, 2009, p. 6585-6587.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Aptamers as a model for functional evaluation of LNA and 2'-amino LNA

AU - Hernandez, Frank J.

AU - Kalra, Neerja

AU - Wengel, Jesper

AU - Vester, Birte

PY - 2009

Y1 - 2009

N2 - The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.

AB - The affinity change upon incorporation of LNA and 2′-amino-LNA monomers into an avidin binding DNA aptamer is described. The kinetic profile of selected modified-aptamer was obtained by surface plasmon resonance experiments and compared with the profile of the parent unmodified DNA aptamer. We report significant improvement of avidin binding affinity by the incorporation of single LNA modifications into the aptamer, and successful incorporation of 2′-amino LNA as a novel monomer in aptamers with potential function as carrier unit for additional molecular entities.

U2 - 10.1016/j.bmcl.2009.10.039

DO - 10.1016/j.bmcl.2009.10.039

M3 - Journal article

VL - 19

SP - 6585

EP - 6587

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 23

ER -