Applying online nano-UHPLC to proteomics

Lasse Gaarde Falkenby, Ole Hørning, Christian Ravnsborg, Jakob Bunkenborg, Jens S. Andersen

Research output: Contribution to conference without publisher/journalPosterResearch

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Abstract

Ultra High Performance Liquid Chromatography (UHPLC) pushes the limits of feasible column designs by allowing higher operational pressure. Migrating from nano-HPLC to nano-UHPLC and coupling directly to a mass spectrometer requires alterations to the setup to allow the increase in pressure. We systematically evaluate experimental parameters such as column dimensions and various stationary phases and how they apply to proteomic analysis.
Original languageEnglish
Publication date6. Jun 2011
Number of pages1
Publication statusPublished - 6. Jun 2011
Event59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics - Denver, CO, United States
Duration: 5. Jun 20119. Jun 2011
Conference number: 59

Conference

Conference59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics
Number59
CountryUnited States
CityDenver, CO
Period05/06/201109/06/2011

Cite this

Falkenby, L. G., Hørning, O., Ravnsborg, C., Bunkenborg, J., & Andersen, J. S. (2011). Applying online nano-UHPLC to proteomics. Poster session presented at 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Denver, CO, United States.
Falkenby, Lasse Gaarde ; Hørning, Ole ; Ravnsborg, Christian ; Bunkenborg, Jakob ; Andersen, Jens S. / Applying online nano-UHPLC to proteomics. Poster session presented at 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Denver, CO, United States.1 p.
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Falkenby, LG, Hørning, O, Ravnsborg, C, Bunkenborg, J & Andersen, JS 2011, 'Applying online nano-UHPLC to proteomics', 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Denver, CO, United States, 05/06/2011 - 09/06/2011.

Applying online nano-UHPLC to proteomics. / Falkenby, Lasse Gaarde; Hørning, Ole; Ravnsborg, Christian; Bunkenborg, Jakob; Andersen, Jens S.

2011. Poster session presented at 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Denver, CO, United States.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Applying online nano-UHPLC to proteomics

AU - Falkenby, Lasse Gaarde

AU - Hørning, Ole

AU - Ravnsborg, Christian

AU - Bunkenborg, Jakob

AU - Andersen, Jens S.

PY - 2011/6/6

Y1 - 2011/6/6

N2 - Ultra High Performance Liquid Chromatography (UHPLC) pushes the limits of feasible column designs by allowing higher operational pressure. Migrating from nano-HPLC to nano-UHPLC and coupling directly to a mass spectrometer requires alterations to the setup to allow the increase in pressure. We systematically evaluate experimental parameters such as column dimensions and various stationary phases and how they apply to proteomic analysis.

AB - Ultra High Performance Liquid Chromatography (UHPLC) pushes the limits of feasible column designs by allowing higher operational pressure. Migrating from nano-HPLC to nano-UHPLC and coupling directly to a mass spectrometer requires alterations to the setup to allow the increase in pressure. We systematically evaluate experimental parameters such as column dimensions and various stationary phases and how they apply to proteomic analysis.

KW - Proteomics

KW - LC-MS/MS

KW - HPLC

M3 - Poster

ER -

Falkenby LG, Hørning O, Ravnsborg C, Bunkenborg J, Andersen JS. Applying online nano-UHPLC to proteomics. 2011. Poster session presented at 59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Denver, CO, United States.