Anammox and partial nitritation in the mainstream of a wastewater treatment plant in a temperate region (Denmark)

Anja Kamp*, Lars Ditlev Mørck Ottosen, Nikolaj Bang Thøgersen, Niels Peter Revsbech, Bo Thamdrup, Mikkel Holmen Andersen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The Marselisborg WWTP (Aarhus, Denmark) fed the mainstream nitrification/denitrification tanks with excess sludge from a sidestream DEMON tank for more than three years to investigate if anammox can supplement conventional nitrification/denitrification in a mainstream of a temperate region. To evaluate this long-term attempt, anammox and also denitrification rates were measured in activated sludge from the main- and sidestream at 10, 20 and 30 C using 15N-labelling (stable isotope) experiments. The results show that anammox contributes by approximately 1% of the total nitrogen removal in the mainstream tanks and that anammox conversion rates there are approximately 800–900 times lower than in the DEMON. A distinct temperature dependence of both anammox and denitrification rates was also confirmed, however, results from different temperatures did not significantly alter relative shares, e.g. anammox rates in activated sludge from the nitrification/denitrification tanks are also negligible at 30 C. This indicates that the anammox bacteria abundance in the nitrification/denitrification tanks is too low to play an important role and that an adaptation to lower temperatures had not occurred. Additional in situ measurements in the nitrification/denitrification tanks further revealed that full nitrification dominates over partial nitritation. Dominant nitritation-anammox is therefore excluded per se and also nitrite shunt activities are not particularly supported.

Original languageEnglish
JournalWater Science and Technology
Volume79
Issue number7
Pages (from-to)1397-1405
ISSN0273-1223
DOIs
Publication statusPublished - 1. Apr 2019

Fingerprint

Denitrification
Nitrification
Wastewater treatment
denitrification
nitrification
activated sludge
Nitrogen removal
wastewater treatment plant
in situ measurement
Temperature
Labeling
nitrite
Isotopes
Bacteria
stable isotope
temperature
sludge
bacterium
rate
nitrogen

Keywords

  • Deammonification
  • DEMON
  • Denitrification
  • Nitrification
  • Nitrite shunt
  • Nitrogen removal

Cite this

Kamp, Anja ; Ottosen, Lars Ditlev Mørck ; Thøgersen, Nikolaj Bang ; Revsbech, Niels Peter ; Thamdrup, Bo ; Andersen, Mikkel Holmen. / Anammox and partial nitritation in the mainstream of a wastewater treatment plant in a temperate region (Denmark). In: Water Science and Technology. 2019 ; Vol. 79, No. 7. pp. 1397-1405.
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abstract = "The Marselisborg WWTP (Aarhus, Denmark) fed the mainstream nitrification/denitrification tanks with excess sludge from a sidestream DEMON tank for more than three years to investigate if anammox can supplement conventional nitrification/denitrification in a mainstream of a temperate region. To evaluate this long-term attempt, anammox and also denitrification rates were measured in activated sludge from the main- and sidestream at 10, 20 and 30 C using 15N-labelling (stable isotope) experiments. The results show that anammox contributes by approximately 1{\%} of the total nitrogen removal in the mainstream tanks and that anammox conversion rates there are approximately 800–900 times lower than in the DEMON. A distinct temperature dependence of both anammox and denitrification rates was also confirmed, however, results from different temperatures did not significantly alter relative shares, e.g. anammox rates in activated sludge from the nitrification/denitrification tanks are also negligible at 30 C. This indicates that the anammox bacteria abundance in the nitrification/denitrification tanks is too low to play an important role and that an adaptation to lower temperatures had not occurred. Additional in situ measurements in the nitrification/denitrification tanks further revealed that full nitrification dominates over partial nitritation. Dominant nitritation-anammox is therefore excluded per se and also nitrite shunt activities are not particularly supported.",
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Anammox and partial nitritation in the mainstream of a wastewater treatment plant in a temperate region (Denmark). / Kamp, Anja; Ottosen, Lars Ditlev Mørck; Thøgersen, Nikolaj Bang; Revsbech, Niels Peter; Thamdrup, Bo; Andersen, Mikkel Holmen.

In: Water Science and Technology, Vol. 79, No. 7, 01.04.2019, p. 1397-1405.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Anammox and partial nitritation in the mainstream of a wastewater treatment plant in a temperate region (Denmark)

AU - Kamp, Anja

AU - Ottosen, Lars Ditlev Mørck

AU - Thøgersen, Nikolaj Bang

AU - Revsbech, Niels Peter

AU - Thamdrup, Bo

AU - Andersen, Mikkel Holmen

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The Marselisborg WWTP (Aarhus, Denmark) fed the mainstream nitrification/denitrification tanks with excess sludge from a sidestream DEMON tank for more than three years to investigate if anammox can supplement conventional nitrification/denitrification in a mainstream of a temperate region. To evaluate this long-term attempt, anammox and also denitrification rates were measured in activated sludge from the main- and sidestream at 10, 20 and 30 C using 15N-labelling (stable isotope) experiments. The results show that anammox contributes by approximately 1% of the total nitrogen removal in the mainstream tanks and that anammox conversion rates there are approximately 800–900 times lower than in the DEMON. A distinct temperature dependence of both anammox and denitrification rates was also confirmed, however, results from different temperatures did not significantly alter relative shares, e.g. anammox rates in activated sludge from the nitrification/denitrification tanks are also negligible at 30 C. This indicates that the anammox bacteria abundance in the nitrification/denitrification tanks is too low to play an important role and that an adaptation to lower temperatures had not occurred. Additional in situ measurements in the nitrification/denitrification tanks further revealed that full nitrification dominates over partial nitritation. Dominant nitritation-anammox is therefore excluded per se and also nitrite shunt activities are not particularly supported.

AB - The Marselisborg WWTP (Aarhus, Denmark) fed the mainstream nitrification/denitrification tanks with excess sludge from a sidestream DEMON tank for more than three years to investigate if anammox can supplement conventional nitrification/denitrification in a mainstream of a temperate region. To evaluate this long-term attempt, anammox and also denitrification rates were measured in activated sludge from the main- and sidestream at 10, 20 and 30 C using 15N-labelling (stable isotope) experiments. The results show that anammox contributes by approximately 1% of the total nitrogen removal in the mainstream tanks and that anammox conversion rates there are approximately 800–900 times lower than in the DEMON. A distinct temperature dependence of both anammox and denitrification rates was also confirmed, however, results from different temperatures did not significantly alter relative shares, e.g. anammox rates in activated sludge from the nitrification/denitrification tanks are also negligible at 30 C. This indicates that the anammox bacteria abundance in the nitrification/denitrification tanks is too low to play an important role and that an adaptation to lower temperatures had not occurred. Additional in situ measurements in the nitrification/denitrification tanks further revealed that full nitrification dominates over partial nitritation. Dominant nitritation-anammox is therefore excluded per se and also nitrite shunt activities are not particularly supported.

KW - Deammonification

KW - DEMON

KW - Denitrification

KW - Nitrification

KW - Nitrite shunt

KW - Nitrogen removal

U2 - 10.2166/wst.2019.141

DO - 10.2166/wst.2019.141

M3 - Journal article

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VL - 79

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EP - 1405

JO - Water Science and Technology

JF - Water Science and Technology

SN - 0273-1223

IS - 7

ER -