Analysis of Secreted Proteins Using SILAC

Jeanette Henningsen, Blagoy Blagoev, Irina Kratchmarova

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Secreted proteins serve a crucial role in the communication between cells, tissues, and organs. Proteins released to the extracellular environment exert their function either locally or at distant points of the organism. Proteins are secreted in a highly dynamic fashion by cells and tissues in the body responding to the stimuli and requirements presented by the extracellular milieu. Characterization of secretomes derived from various cell types has been performed using different quantitative mass spectrometry-based proteomics strategies, several of them taking advantage of labeling with stable isotopes. Here, we describe the use of Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for the quantitative analysis of the skeletal muscle secretome during myogenesis.

Original languageEnglish
JournalMethods in Molecular Biology
Volume1188
Pages (from-to)313-26
Number of pages14
ISSN1064-3745
DOIs
Publication statusPublished - 2014

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