Isobaric labeling has become an essential method for quantitative mass spectrometry based experiments. This technique allows high-throughput proteomics while providing reasonable coverage of protein measurements across multiple samples. Here, the analysis of isobarically labeled mass spectrometry data with a special focus on quality control and potential pitfalls is discussed. The protocol is based on our fully integrated IsoProt workflow. The concepts discussed are nevertheless applicable to the analysis of any isobarically labeled experiment using alternative computational tools and algorithms.
|Title of host publication||Proteomics Data Analysis|
|Place of Publication||New York|
|ISBN (Print)||978-1-0716-1640-6, 978-1-0716-1643-7|
|Publication status||Published - 2021|
|Series||Methods in Molecular Biology|
Bibliographical notePublisher Copyright:
© 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Isobaric labeling
- Mass spectrometry
- Quantitative proteomics