Abstract
| Original language | English |
|---|---|
| Title of host publication | LIPIDS, VOL 108 |
| Editors | G DiPaolo, MR Wenk |
| Number of pages | 27 |
| Volume | 108 |
| Publication date | 2012 |
| Pages | 367-393 |
| DOIs | |
| Publication status | Published - 2012 |
| Series | Methods in Cell Biology |
|---|---|
| Volume | 108 |
| ISSN | 0091-679X |
Cite this
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Analysis of Cholesterol Trafficking with Fluorescent Probes. / Maxfield, Frederick R.; Wustner, Daniel.
LIPIDS, VOL 108. ed. / G DiPaolo; MR Wenk. Vol. 108 2012. p. 367-393 (Methods in Cell Biology, Vol. 108).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
TY - CHAP
T1 - Analysis of Cholesterol Trafficking with Fluorescent Probes
AU - Maxfield, Frederick R.
AU - Wustner, Daniel
PY - 2012
Y1 - 2012
N2 - Cholesterol plays an important role in determining the biophysical properties of biological membranes, and its concentration is tightly controlled by homeostatic processes. The intracellular transport of cholesterol among organelles is a key part of the homeostatic mechanism, but sterol transport processes are not well understood. Fluorescence microscopy is a valuable tool for studying intracellular transport processes, but this method can be challenging for lipid molecules because addition of a fluorophore may alter the properties of the molecule greatly. We discuss the use of fluorescent molecules that can bind to cholesterol to reveal its distribution in cells. We also discuss the use of intrinsically fluorescent sterols that closely mimic cholesterol, as well as some minimally modified fluorophore-labeled sterols. Methods for imaging these sterols by conventional fluorescence microscopy and by multiphoton microscopy are described. Some label-free methods for imaging cholesterol itself are also discussed briefly.
AB - Cholesterol plays an important role in determining the biophysical properties of biological membranes, and its concentration is tightly controlled by homeostatic processes. The intracellular transport of cholesterol among organelles is a key part of the homeostatic mechanism, but sterol transport processes are not well understood. Fluorescence microscopy is a valuable tool for studying intracellular transport processes, but this method can be challenging for lipid molecules because addition of a fluorophore may alter the properties of the molecule greatly. We discuss the use of fluorescent molecules that can bind to cholesterol to reveal its distribution in cells. We also discuss the use of intrinsically fluorescent sterols that closely mimic cholesterol, as well as some minimally modified fluorophore-labeled sterols. Methods for imaging these sterols by conventional fluorescence microscopy and by multiphoton microscopy are described. Some label-free methods for imaging cholesterol itself are also discussed briefly.
U2 - 10.1016/B978-0-12-386487-1.00017-1
DO - 10.1016/B978-0-12-386487-1.00017-1
M3 - Book chapter
C2 - 22325611
VL - 108
T3 - Methods in Cell Biology
SP - 367
EP - 393
BT - LIPIDS, VOL 108
A2 - DiPaolo, G
A2 - Wenk, MR
ER -