Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability

Marie Willaing Johannsen, Jesper Wengel, Michael Chr. Wamberg, Lia Crispino, Neerja Kalra

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearch

Abstract

The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability.
Original languageEnglish
Publication date2010
Number of pages1
Publication statusPublished - 2010

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