Amino-acid sequence of the vitamin-K-dependent part of protein Z

P Højrup, P Roepstorff, T E Petersen

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Abstract

After tryptic digestion of protein Z a peptide corresponding to residues 1-43 has been isolated and sequenced. Smaller peptides were generated by treatment of the peptide with pepsin and then separated by high-performance liquid chromatography. The purity of the peptides was sufficient for mass spectrometric measurement. Thirteen gamma-carboxyglutamic acid residues have been identified and the peptide shows strong homology to the corresponding domains of the classical vitamin-K-dependent blood clotting proteins.

Original languageEnglish
JournalEuropean Journal of Biochemistry
Volume126
Issue number2
Pages (from-to)343-8
Number of pages6
ISSN0014-2956
DOIs
Publication statusPublished - Aug 1982

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Keywords

  • Amino Acid Sequence
  • Animals
  • Blood Proteins/isolation & purification
  • Cattle
  • Chromatography, High Pressure Liquid
  • Mass Spectrometry
  • Peptide Fragments/isolation & purification
  • Vitamin K/pharmacology

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