Amino acid sequence of acyl-CoA-binding protein from cow liver

J Mikkelsen; P Højrup; P F Nielsen; P Roepstorff; J Knudsen

doi:10.1042/bj2450857

Amino acid sequence of acyl-CoA-binding protein from cow liver

J Mikkelsen, P Højrup, P F Nielsen, P Roepstorff, J Knudsen

Department of Biochemistry and Molecular Biology

University of Southern Denmark

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Acyl-CoA-binding protein from bovine liver was purified with the use of reverse-phase h.p.l.c. in the final step. The complete amino acid sequence was determined by using a combination of gas-phase Edman degradation and electron-impact and fast-atom-bombardment mass spectrometry. The sequence was confirmed by determination of the Mr by plasma-desorption time-of-flight mass spectrometry.

Original language	English
Journal	Biochemical Journal
Volume	245
Issue number	3
Pages (from-to)	857-61
Number of pages	5
ISSN	0264-6021
DOIs	https://doi.org/10.1042/bj2450857
Publication status	Published - 1. Aug 1987

Keywords

Amino Acid Sequence
Animals
Carrier Proteins/isolation & purification
Cattle
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Fatty Acid-Binding Proteins
Female
Liver/analysis
Mass Spectrometry
Neoplasm Proteins
Peptide Fragments/analysis

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10.1042/bj2450857

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title = "Amino acid sequence of acyl-CoA-binding protein from cow liver",

abstract = "Acyl-CoA-binding protein from bovine liver was purified with the use of reverse-phase h.p.l.c. in the final step. The complete amino acid sequence was determined by using a combination of gas-phase Edman degradation and electron-impact and fast-atom-bombardment mass spectrometry. The sequence was confirmed by determination of the Mr by plasma-desorption time-of-flight mass spectrometry.",

keywords = "Amino Acid Sequence, Animals, Carrier Proteins/isolation & purification, Cattle, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Fatty Acid-Binding Proteins, Female, Liver/analysis, Mass Spectrometry, Neoplasm Proteins, Peptide Fragments/analysis",

author = "J Mikkelsen and P H{\o}jrup and Nielsen, {P F} and P Roepstorff and J Knudsen",

year = "1987",

month = aug,

day = "1",

doi = "10.1042/bj2450857",

language = "English",

volume = "245",

pages = "857--61",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press",

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TY - JOUR

T1 - Amino acid sequence of acyl-CoA-binding protein from cow liver

AU - Mikkelsen, J

AU - Højrup, P

AU - Nielsen, P F

AU - Roepstorff, P

AU - Knudsen, J

PY - 1987/8/1

Y1 - 1987/8/1

N2 - Acyl-CoA-binding protein from bovine liver was purified with the use of reverse-phase h.p.l.c. in the final step. The complete amino acid sequence was determined by using a combination of gas-phase Edman degradation and electron-impact and fast-atom-bombardment mass spectrometry. The sequence was confirmed by determination of the Mr by plasma-desorption time-of-flight mass spectrometry.

AB - Acyl-CoA-binding protein from bovine liver was purified with the use of reverse-phase h.p.l.c. in the final step. The complete amino acid sequence was determined by using a combination of gas-phase Edman degradation and electron-impact and fast-atom-bombardment mass spectrometry. The sequence was confirmed by determination of the Mr by plasma-desorption time-of-flight mass spectrometry.

KW - Amino Acid Sequence

KW - Animals

KW - Carrier Proteins/isolation & purification

KW - Cattle

KW - Chromatography, High Pressure Liquid

KW - Electrophoresis, Polyacrylamide Gel

KW - Fatty Acid-Binding Proteins

KW - Female

KW - Liver/analysis

KW - Mass Spectrometry

KW - Neoplasm Proteins

KW - Peptide Fragments/analysis

U2 - 10.1042/bj2450857

DO - 10.1042/bj2450857

M3 - Journal article

C2 - 3663196

SN - 0264-6021

VL - 245

SP - 857

EP - 861

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Amino acid sequence of acyl-CoA-binding protein from cow liver

Abstract

Keywords

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