Alpha-L-locked nucleic acid-modified antisense oligonucleotides induce efficient splice modulation in vitro

Prithi Raguraman, Tao Wang, Lixia Ma, Per Trolle Jørgensen, Jesper Wengel, Rakesh N. Veedu*

*Corresponding author for this work

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Abstract

Alpha-L-Locked nucleic acid (α-L-LNA) is a stereoisomeric analogue of locked nucleic acid (LNA), which possesses excellent biophysical properties and also exhibits high target binding affinity to complementary oligonucleotide sequences and resistance to nuclease degradations. Therefore, α-L-LNA nucleotides could be utilised to develop stable antisense oligonucleotides (AO), which can be truncated without compromising the integrity and efficacy of the AO. In this study, we explored the potential of α-L-LNA nucleotides-modified antisense oligonucleotides to modulate splicing by inducing Dmd exon-23 skipping in mdx mouse myoblasts in vitro. For this purpose, we have synthesised and systematically evaluated the efficacy of α-L-LNA-modified 2′-O-methyl phosphorothioate (2′-OMePS) AOs of three different sizes including 20mer, 18mer and 16mer AOs in parallel to fully-modified 2′-OMePS control AOs. Our results demonstrated that the 18mer and 16mer truncated AO variants showed slightly better exon-skipping efficacy when compared with the fully-23 modified 2′-OMePS control AOs, in addition to showing low cytotoxicity. As there was no previous report on using α-L-LNA-modified AOs in splice modulation, we firmly believe that this initial study could be beneficial to further explore and expand the scope of α-L-LNA-modified AO therapeutic molecules.

Original languageEnglish
Article number2434
JournalInternational Journal of Molecular Sciences
Volume21
Issue number7
Number of pages12
ISSN1661-6596
DOIs
Publication statusPublished - 2020

Keywords

  • Antisense oligonucleotides
  • DMD
  • Locked nucleic acids
  • α-L-LNA

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