Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis

Sam Shepherd*, Emily Jevons, Juliette Strauss, Kasper Degn Gejl, Kurt Jensen, Lars Grøndahl Hvid, Ulrik Frandsen, Kent Sahlin, Niels Ørtenblad

*Corresponding author for this work

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

Abstract

Intramuscular triglyceride (IMTG) is an important substrate during moderateintensity exercise, but providing a high carbohydrate (CHO) diet following exercise impairs IMTG resynthesis. Restricting CHO intake in the post-exercise period may augment the adaptive response to exercise, but whether this strategy enhances IMTG resynthesis is not known. Furthermore, because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote IMTG storage, their distribution and interaction with LD may determine their role in post-exercise IMTG resynthesis. Purpose: To determine the effect of acutely restricting CHO during recovery from prolonged exercise on IMTG resynthesis and PLIN protein dynamics. Methods: 14 male triathletes (27±1 y, 66.5±1.3 ml.kg-1.min-1) completed 4 h of cycling at ~56% VO2max. In the initial 4 h period following exercise 7 participants consumed a high CHO diet whereas 7 participants consumed only water. For the remaining recovery period (20 h) all participants received the same CHO-rich diet. Muscle samples collected pre and post-exercise, and 4 and 24 h post-exercise were analysed using confocal immunofluorescence microscopy to determine muscle fibre type-specific IMTG content and PLIN localisation to LDs. Results: Exercise reduced IMTG content in type I fibres (-53%, P<0.05), with LD both associated and not associated with PLIN2, PLIN3, and PLIN5 being used. During recovery, IMTG content tended to increase in type I fibres after 4 h in the water condition only (+63%, P=0.076), and at 24 h IMTG content was similar to pre-exercise levels (P=0.987). Despite no change in PLIN protein content, the number of LD with PLIN2 or PLIN3 associated tended to increase during recovery in type I fibres only (P=0.08), and to a similar extent between conditions, whereas the number of LD with PLIN5 associated only increased in the CHO condition (P=0.002). Conclusion: Acute restriction of CHO during recovery from prolonged exercise has a tendency to initially enhance IMTG resynthesis, although a CHO-rich diet does not impair overall IMTG resynthesis 24 h later. A redistribution of PLIN proteins appears to support post-exercise IMTG resynthesis, but the predominant PLIN isoform supporting post-exercise IMTG storage may be dependent on post-exercise CHO availability
Original languageEnglish
Publication date30. May 2019
Publication statusPublished - 30. May 2019
EventACSM Orlando - Florida, Orlando, United States
Duration: 28. May 20191. Jun 2019
http://www.acsm.org/annual-meeting/future-past-meetings/2019-orlando-fl

Conference

ConferenceACSM Orlando
LocationFlorida
CountryUnited States
CityOrlando
Period28/05/201901/06/2019
Internet address

Cite this

Shepherd, S., Jevons, E., Strauss, J., Gejl, K. D., Jensen, K., Hvid, L. G., ... Ørtenblad, N. (2019). Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis. Abstract from ACSM Orlando, Orlando, United States.
Shepherd, Sam ; Jevons, Emily ; Strauss, Juliette ; Gejl, Kasper Degn ; Jensen, Kurt ; Hvid, Lars Grøndahl ; Frandsen, Ulrik ; Sahlin, Kent ; Ørtenblad, Niels. / Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis. Abstract from ACSM Orlando, Orlando, United States.
@conference{41eae1fcc279426cb6897f86e90ff41b,
title = "Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis",
abstract = "Intramuscular triglyceride (IMTG) is an important substrate during moderateintensity exercise, but providing a high carbohydrate (CHO) diet following exercise impairs IMTG resynthesis. Restricting CHO intake in the post-exercise period may augment the adaptive response to exercise, but whether this strategy enhances IMTG resynthesis is not known. Furthermore, because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote IMTG storage, their distribution and interaction with LD may determine their role in post-exercise IMTG resynthesis. Purpose: To determine the effect of acutely restricting CHO during recovery from prolonged exercise on IMTG resynthesis and PLIN protein dynamics. Methods: 14 male triathletes (27±1 y, 66.5±1.3 ml.kg-1.min-1) completed 4 h of cycling at ~56{\%} VO2max. In the initial 4 h period following exercise 7 participants consumed a high CHO diet whereas 7 participants consumed only water. For the remaining recovery period (20 h) all participants received the same CHO-rich diet. Muscle samples collected pre and post-exercise, and 4 and 24 h post-exercise were analysed using confocal immunofluorescence microscopy to determine muscle fibre type-specific IMTG content and PLIN localisation to LDs. Results: Exercise reduced IMTG content in type I fibres (-53{\%}, P<0.05), with LD both associated and not associated with PLIN2, PLIN3, and PLIN5 being used. During recovery, IMTG content tended to increase in type I fibres after 4 h in the water condition only (+63{\%}, P=0.076), and at 24 h IMTG content was similar to pre-exercise levels (P=0.987). Despite no change in PLIN protein content, the number of LD with PLIN2 or PLIN3 associated tended to increase during recovery in type I fibres only (P=0.08), and to a similar extent between conditions, whereas the number of LD with PLIN5 associated only increased in the CHO condition (P=0.002). Conclusion: Acute restriction of CHO during recovery from prolonged exercise has a tendency to initially enhance IMTG resynthesis, although a CHO-rich diet does not impair overall IMTG resynthesis 24 h later. A redistribution of PLIN proteins appears to support post-exercise IMTG resynthesis, but the predominant PLIN isoform supporting post-exercise IMTG storage may be dependent on post-exercise CHO availability",
author = "Sam Shepherd and Emily Jevons and Juliette Strauss and Gejl, {Kasper Degn} and Kurt Jensen and Hvid, {Lars Gr{\o}ndahl} and Ulrik Frandsen and Kent Sahlin and Niels {\O}rtenblad",
year = "2019",
month = "5",
day = "30",
language = "English",
note = "null ; Conference date: 28-05-2019 Through 01-06-2019",
url = "http://www.acsm.org/annual-meeting/future-past-meetings/2019-orlando-fl",

}

Shepherd, S, Jevons, E, Strauss, J, Gejl, KD, Jensen, K, Hvid, LG, Frandsen, U, Sahlin, K & Ørtenblad, N 2019, 'Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis', ACSM Orlando, Orlando, United States, 28/05/2019 - 01/06/2019.

Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis. / Shepherd, Sam; Jevons, Emily; Strauss, Juliette; Gejl, Kasper Degn; Jensen, Kurt; Hvid, Lars Grøndahl; Frandsen, Ulrik; Sahlin, Kent; Ørtenblad, Niels.

2019. Abstract from ACSM Orlando, Orlando, United States.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis

AU - Shepherd, Sam

AU - Jevons, Emily

AU - Strauss, Juliette

AU - Gejl, Kasper Degn

AU - Jensen, Kurt

AU - Hvid, Lars Grøndahl

AU - Frandsen, Ulrik

AU - Sahlin, Kent

AU - Ørtenblad, Niels

PY - 2019/5/30

Y1 - 2019/5/30

N2 - Intramuscular triglyceride (IMTG) is an important substrate during moderateintensity exercise, but providing a high carbohydrate (CHO) diet following exercise impairs IMTG resynthesis. Restricting CHO intake in the post-exercise period may augment the adaptive response to exercise, but whether this strategy enhances IMTG resynthesis is not known. Furthermore, because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote IMTG storage, their distribution and interaction with LD may determine their role in post-exercise IMTG resynthesis. Purpose: To determine the effect of acutely restricting CHO during recovery from prolonged exercise on IMTG resynthesis and PLIN protein dynamics. Methods: 14 male triathletes (27±1 y, 66.5±1.3 ml.kg-1.min-1) completed 4 h of cycling at ~56% VO2max. In the initial 4 h period following exercise 7 participants consumed a high CHO diet whereas 7 participants consumed only water. For the remaining recovery period (20 h) all participants received the same CHO-rich diet. Muscle samples collected pre and post-exercise, and 4 and 24 h post-exercise were analysed using confocal immunofluorescence microscopy to determine muscle fibre type-specific IMTG content and PLIN localisation to LDs. Results: Exercise reduced IMTG content in type I fibres (-53%, P<0.05), with LD both associated and not associated with PLIN2, PLIN3, and PLIN5 being used. During recovery, IMTG content tended to increase in type I fibres after 4 h in the water condition only (+63%, P=0.076), and at 24 h IMTG content was similar to pre-exercise levels (P=0.987). Despite no change in PLIN protein content, the number of LD with PLIN2 or PLIN3 associated tended to increase during recovery in type I fibres only (P=0.08), and to a similar extent between conditions, whereas the number of LD with PLIN5 associated only increased in the CHO condition (P=0.002). Conclusion: Acute restriction of CHO during recovery from prolonged exercise has a tendency to initially enhance IMTG resynthesis, although a CHO-rich diet does not impair overall IMTG resynthesis 24 h later. A redistribution of PLIN proteins appears to support post-exercise IMTG resynthesis, but the predominant PLIN isoform supporting post-exercise IMTG storage may be dependent on post-exercise CHO availability

AB - Intramuscular triglyceride (IMTG) is an important substrate during moderateintensity exercise, but providing a high carbohydrate (CHO) diet following exercise impairs IMTG resynthesis. Restricting CHO intake in the post-exercise period may augment the adaptive response to exercise, but whether this strategy enhances IMTG resynthesis is not known. Furthermore, because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote IMTG storage, their distribution and interaction with LD may determine their role in post-exercise IMTG resynthesis. Purpose: To determine the effect of acutely restricting CHO during recovery from prolonged exercise on IMTG resynthesis and PLIN protein dynamics. Methods: 14 male triathletes (27±1 y, 66.5±1.3 ml.kg-1.min-1) completed 4 h of cycling at ~56% VO2max. In the initial 4 h period following exercise 7 participants consumed a high CHO diet whereas 7 participants consumed only water. For the remaining recovery period (20 h) all participants received the same CHO-rich diet. Muscle samples collected pre and post-exercise, and 4 and 24 h post-exercise were analysed using confocal immunofluorescence microscopy to determine muscle fibre type-specific IMTG content and PLIN localisation to LDs. Results: Exercise reduced IMTG content in type I fibres (-53%, P<0.05), with LD both associated and not associated with PLIN2, PLIN3, and PLIN5 being used. During recovery, IMTG content tended to increase in type I fibres after 4 h in the water condition only (+63%, P=0.076), and at 24 h IMTG content was similar to pre-exercise levels (P=0.987). Despite no change in PLIN protein content, the number of LD with PLIN2 or PLIN3 associated tended to increase during recovery in type I fibres only (P=0.08), and to a similar extent between conditions, whereas the number of LD with PLIN5 associated only increased in the CHO condition (P=0.002). Conclusion: Acute restriction of CHO during recovery from prolonged exercise has a tendency to initially enhance IMTG resynthesis, although a CHO-rich diet does not impair overall IMTG resynthesis 24 h later. A redistribution of PLIN proteins appears to support post-exercise IMTG resynthesis, but the predominant PLIN isoform supporting post-exercise IMTG storage may be dependent on post-exercise CHO availability

M3 - Conference abstract for conference

ER -

Shepherd S, Jevons E, Strauss J, Gejl KD, Jensen K, Hvid LG et al. Acute Carbohydrate Restriction During Recovery From Prolonged Exercise Enhances Intramuscular Triglyceride Resynthesis. 2019. Abstract from ACSM Orlando, Orlando, United States.