Aerobic and anaerobic methane oxidation in a seasonally anoxic basin

Herdís G.R. Steinsdóttir*, Clemens Schauberger, Snehit Mhatre, Bo Thamdrup, Laura A. Bristow

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Shallow coastal waters are dynamic environments that dominate global marine methane emissions. Particularly high methane concentrations are found in seasonally anoxic waters, which are spreading in eutrophic coastal systems, potentially leading to increased methane emissions to the atmosphere. Here we explore how the seasonal development of anoxia influenced methane concentrations, rates of methane oxidation, and the community composition of methanotrophs in the shallow eutrophic water column of Mariager Fjord, Denmark. Our results show the development of steep concentration gradients toward the oxic–anoxic interface as methane accumulated to 1.4 μM in anoxic bottom waters. Yet, the fjord possessed an efficient microbial methane filter near the oxic–anoxic interface that responded to the increasing methane flux. In experimental incubations, methane oxidation near the oxic–anoxic interface proceeded both aerobically and anaerobically with nearly equal efficiency reaching turnover rates as high as 0.6 and 0.8 d−1, respectively, and was seemingly mediated by members of the Methylococcales belonging to the Deep Sea-1 clade. Throughout the period, both aerobic and anaerobic methane oxidation rates were high enough to consume the estimated methane flux. Thus, our results indicate that seasonal anoxia did not increase methane emissions.

Original languageEnglish
JournalLimnology and Oceanography
Volume67
Issue number6
Pages (from-to)1257-1273
ISSN0024-3590
DOIs
Publication statusPublished - Jun 2022

Bibliographical note

Funding Information:
We are grateful to Gert Pedersen, Johannes Christian Thorhauge and Lars Peter Jørgensen for enabling and assisting with sampling in Mariager Fjord, and Anders Soltau Barnewitz, Lene Jakobsen and Louise Reinbach Hansen for help with sampling and chemical analysis. We thank Erik Laursen for carrying out PCR analysis and Carmen Czepe at the Next Generation Sequencing Facility of the Vienna Biocenter ( www.vbcf.ac.at ) for sequencing. We thank two anonymous reviewers for their constructive comments. This work was supported by the European Research Council (European Research Council (ERC) Advanced Grant 695599 NOVAMOX to BT).

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