Abstract
Purpose: Adipose derived mesenchymal stem cells (ADMSCs) may be osteogenic, may generate neoangiogenisis and may be progenitors for differentiated osteoblast mineralization. Titanium granules may be suitable as carriers for these cells. The aim was to demonstrate the osteogenic potential of ADMSCs and the effect of porous non-oxidized (Ti) and oxidized titanium (TiO 2) granules as carriers for ADMSCs mineralization in vitro. Materials and methods: ADMSCs were isolated, cultivated in osteoblast medium and evaluated for alkaline phosphatase (ALP) assay, RNA isolation, and ALP staining. Osteoblast in vitro mineralization cells without granules or seeded on Ti or TiO 2 granules were evaluated for Alizarin Red assay and RNA isolation for later gene expressing. Results: ADMSCs express osteoblastic lineage genes, CBFA-1 and stain strongly for ALP. Mineralization was significantly higher for cells seeded on TiO 2 than on Ti granules or pure cells. Expression of ALPL and RUNX2 was significantly higher for cells seeded on TiO 2 granules and expression of COL1α1 for pure cells was significantly higher than for cells seeded on granules. Conclusion: ADMSCs have osteogenic potential. Mineralization was significantly high when cells were seeded on TiO 2 granules. TiO 2 granules may be used as carriers for adipose derived mesenchymal osteoblastic cells from laboratory bench to the patient.
Original language | English |
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Journal | Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery |
Volume | 41 |
Issue number | 8 |
Pages (from-to) | e213-e220 |
Number of pages | 8 |
ISSN | 1010-5182 |
DOIs | |
Publication status | Published - Dec 2013 |
Keywords
- Adipose mesenchymal stem cells
- Drug carriers
- Mineralization
- Tissue engineering
- Titanium
- Osteogenesis/physiology
- Coloring Agents
- Titanium/chemistry
- Mesenchymal Stem Cells/physiology
- Cell Differentiation/physiology
- Cell Culture Techniques
- Calcification, Physiologic/physiology
- Neovascularization, Physiologic/physiology
- Biocompatible Materials/chemistry
- Collagen Type I/analysis
- Osteoblasts/physiology
- Mice, Inbred Strains
- Alkaline Phosphatase/analysis
- Cell Lineage
- Core Binding Factor Alpha 1 Subunit/analysis
- Animals
- Anthraquinones
- Adipose Tissue/cytology
- Tissue Scaffolds/chemistry
- Mice
- Mice, Inbred BALB C
- Porosity