Acoustic characterisation of pH dependant reversible micellar casein aggregation

M. J. Francis*, Z. J. Glover, Q. Yu, M. J. Povey, M. J. Holmes

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Ultrasound and light-based measurements were used to monitor changes in agitated and quiescent solution characteristics such as particle size distribution during casein aggregation and redistribution in a model colloidal system. The precipitation of casein micelles was controlled by adjusting the pH of the solution to alter the stability of the micelles. The studied dispersions were prepared at high pH, taken through the isoelectric point to precipitate and then re-dispersed by further lowering the pH at specific intervals. The isoelectric point was determined by zeta potential measurement at each specific pH interval. The isoelectric point of the casein dispersions was in the region of pH 4.3–4.7. Changes in the particle size of this reversible casein system were successfully measured acoustically. The dissolution rate and solution kinetics were subsequently monitored in a wide pH range using a combination of acoustic and light-based methods.

Original languageEnglish
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume568
Pages (from-to)259-265
ISSN0927-7757
DOIs
Publication statusPublished - 2019

Fingerprint

Casein
Caseins
Agglomeration
Acoustics
acoustics
Micelles
Dispersions
micelles
Zeta potential
Particle size analysis
intervals
Precipitates
Dissolution
Ultrasonics
Particle size
particle size distribution
Kinetics
precipitates
dissolving
adjusting

Keywords

  • Acoustic spectroscopy
  • Aggregation
  • Casein
  • Ultrasound

Cite this

@article{7881191cde924985b79031d5e517acdb,
title = "Acoustic characterisation of pH dependant reversible micellar casein aggregation",
abstract = "Ultrasound and light-based measurements were used to monitor changes in agitated and quiescent solution characteristics such as particle size distribution during casein aggregation and redistribution in a model colloidal system. The precipitation of casein micelles was controlled by adjusting the pH of the solution to alter the stability of the micelles. The studied dispersions were prepared at high pH, taken through the isoelectric point to precipitate and then re-dispersed by further lowering the pH at specific intervals. The isoelectric point was determined by zeta potential measurement at each specific pH interval. The isoelectric point of the casein dispersions was in the region of pH 4.3–4.7. Changes in the particle size of this reversible casein system were successfully measured acoustically. The dissolution rate and solution kinetics were subsequently monitored in a wide pH range using a combination of acoustic and light-based methods.",
keywords = "Acoustic spectroscopy, Aggregation, Casein, Ultrasound",
author = "Francis, {M. J.} and Glover, {Z. J.} and Q. Yu and Povey, {M. J.} and Holmes, {M. J.}",
year = "2019",
doi = "10.1016/j.colsurfa.2019.02.026",
language = "English",
volume = "568",
pages = "259--265",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",

}

Acoustic characterisation of pH dependant reversible micellar casein aggregation. / Francis, M. J.; Glover, Z. J.; Yu, Q.; Povey, M. J.; Holmes, M. J.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 568, 2019, p. 259-265.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Acoustic characterisation of pH dependant reversible micellar casein aggregation

AU - Francis, M. J.

AU - Glover, Z. J.

AU - Yu, Q.

AU - Povey, M. J.

AU - Holmes, M. J.

PY - 2019

Y1 - 2019

N2 - Ultrasound and light-based measurements were used to monitor changes in agitated and quiescent solution characteristics such as particle size distribution during casein aggregation and redistribution in a model colloidal system. The precipitation of casein micelles was controlled by adjusting the pH of the solution to alter the stability of the micelles. The studied dispersions were prepared at high pH, taken through the isoelectric point to precipitate and then re-dispersed by further lowering the pH at specific intervals. The isoelectric point was determined by zeta potential measurement at each specific pH interval. The isoelectric point of the casein dispersions was in the region of pH 4.3–4.7. Changes in the particle size of this reversible casein system were successfully measured acoustically. The dissolution rate and solution kinetics were subsequently monitored in a wide pH range using a combination of acoustic and light-based methods.

AB - Ultrasound and light-based measurements were used to monitor changes in agitated and quiescent solution characteristics such as particle size distribution during casein aggregation and redistribution in a model colloidal system. The precipitation of casein micelles was controlled by adjusting the pH of the solution to alter the stability of the micelles. The studied dispersions were prepared at high pH, taken through the isoelectric point to precipitate and then re-dispersed by further lowering the pH at specific intervals. The isoelectric point was determined by zeta potential measurement at each specific pH interval. The isoelectric point of the casein dispersions was in the region of pH 4.3–4.7. Changes in the particle size of this reversible casein system were successfully measured acoustically. The dissolution rate and solution kinetics were subsequently monitored in a wide pH range using a combination of acoustic and light-based methods.

KW - Acoustic spectroscopy

KW - Aggregation

KW - Casein

KW - Ultrasound

U2 - 10.1016/j.colsurfa.2019.02.026

DO - 10.1016/j.colsurfa.2019.02.026

M3 - Journal article

AN - SCOPUS:85061640606

VL - 568

SP - 259

EP - 265

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

ER -