Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

Olga A. Mirgorodskaya, Roman Körner*, Yuri P. Kozmin, Peter Roepstorff

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Amino acid analysis is among the most accurate methods for absolute quantification of proteins and peptides. Here we combine acid hydrolysis with the addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10 fmol of protein standards with errors below 10% has been demonstrated using this method.

Original languageEnglish
Title of host publicationAmino Acid Analysis : Methods and Protocols
Number of pages6
Volume2030
PublisherHumana Press
Publication date2019
Pages11-16
Chapter2
ISBN (Print)978-1-4939-9638-4
ISBN (Electronic)978-1-4939-9639-1
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume2030
ISSN1064-3745

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Hydrolysis
Amino Acids
Acids
Proteins
Peptides

Keywords

  • Absolute protein quantitation
  • Amino acid analysis
  • Isotope-labeled amino acids
  • MALDI
  • Mass spectrometry
  • Quantitative mass spectrometry

Cite this

Mirgorodskaya, O. A., Körner, R., Kozmin, Y. P., & Roepstorff, P. (2019). Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. In Amino Acid Analysis: Methods and Protocols (Vol. 2030, pp. 11-16). Humana Press. Methods in Molecular Biology, Vol.. 2030 https://doi.org/10.1007/978-1-4939-9639-1_2
Mirgorodskaya, Olga A. ; Körner, Roman ; Kozmin, Yuri P. ; Roepstorff, Peter. / Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. Amino Acid Analysis: Methods and Protocols. Vol. 2030 Humana Press, 2019. pp. 11-16 (Methods in Molecular Biology, Vol. 2030).
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Mirgorodskaya, OA, Körner, R, Kozmin, YP & Roepstorff, P 2019, Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. in Amino Acid Analysis: Methods and Protocols. vol. 2030, Humana Press, Methods in Molecular Biology, vol. 2030, pp. 11-16. https://doi.org/10.1007/978-1-4939-9639-1_2

Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. / Mirgorodskaya, Olga A.; Körner, Roman; Kozmin, Yuri P.; Roepstorff, Peter.

Amino Acid Analysis: Methods and Protocols. Vol. 2030 Humana Press, 2019. p. 11-16 (Methods in Molecular Biology, Vol. 2030).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

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Mirgorodskaya OA, Körner R, Kozmin YP, Roepstorff P. Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry. In Amino Acid Analysis: Methods and Protocols. Vol. 2030. Humana Press. 2019. p. 11-16. (Methods in Molecular Biology, Vol. 2030). https://doi.org/10.1007/978-1-4939-9639-1_2