A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro

Muwan Chen, Wenzhou Feng, Hui Cao, Lijin Zou, Chungui Chen, Anette Baatrup, Anne Bay Nielsen, Haisheng Li, Moustapha Kassem, Xuenong Zou, Cody Bünger

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50% inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs.
Original languageEnglish
JournalJournal of Ethnopharmacology
Volume125
Issue number1
Pages (from-to)75-82
Number of pages7
ISSN0378-8741
DOIs
Publication statusPublished - 17. Aug 2009

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Mesenchymal Stromal Cells
Inhibitory Concentration 50
Alkaline Phosphatase
Cell Proliferation
In Vitro Techniques
Osteopontin
Osteocalcin
Human Activities
Minerals
Real-Time Polymerase Chain Reaction
Cell Survival
Down-Regulation

Keywords

  • Alkaline Phosphatase
  • Cell Differentiation
  • Cell Proliferation
  • Culture Media
  • Gene Expression Regulation
  • Humans
  • Medicine, Chinese Traditional
  • Mesenchymal Stem Cells
  • Osteoblasts
  • Plant Extracts

Cite this

Chen, Muwan ; Feng, Wenzhou ; Cao, Hui ; Zou, Lijin ; Chen, Chungui ; Baatrup, Anette ; Nielsen, Anne Bay ; Li, Haisheng ; Kassem, Moustapha ; Zou, Xuenong ; Bünger, Cody. / A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro. In: Journal of Ethnopharmacology. 2009 ; Vol. 125, No. 1. pp. 75-82.
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title = "A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro",
abstract = "AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50{\%} inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs.",
keywords = "Alkaline Phosphatase, Cell Differentiation, Cell Proliferation, Culture Media, Gene Expression Regulation, Humans, Medicine, Chinese Traditional, Mesenchymal Stem Cells, Osteoblasts, Plant Extracts",
author = "Muwan Chen and Wenzhou Feng and Hui Cao and Lijin Zou and Chungui Chen and Anette Baatrup and Nielsen, {Anne Bay} and Haisheng Li and Moustapha Kassem and Xuenong Zou and Cody B{\"u}nger",
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A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro. / Chen, Muwan; Feng, Wenzhou; Cao, Hui; Zou, Lijin; Chen, Chungui; Baatrup, Anette; Nielsen, Anne Bay; Li, Haisheng; Kassem, Moustapha; Zou, Xuenong; Bünger, Cody.

In: Journal of Ethnopharmacology, Vol. 125, No. 1, 17.08.2009, p. 75-82.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A traditional Chinese medicine formula extracts stimulate proliferation and inhibit mineralization of human mesenchymal stem cells in vitro

AU - Chen, Muwan

AU - Feng, Wenzhou

AU - Cao, Hui

AU - Zou, Lijin

AU - Chen, Chungui

AU - Baatrup, Anette

AU - Nielsen, Anne Bay

AU - Li, Haisheng

AU - Kassem, Moustapha

AU - Zou, Xuenong

AU - Bünger, Cody

PY - 2009/8/17

Y1 - 2009/8/17

N2 - AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50% inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs.

AB - AIM OF THE STUDY: To investigate the effects of a traditional Chinese medicine (TCM) formula extract, named as ZD-I, on the proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro. MATERIALS AND METHODS: When hMSCs cultivated in the basal medium with ZD-I, cell viability was assessed by MTT assay and cellular proliferation was assessed by SYBR green I assay. The effects of ZD-I on osteogenic differentiation of hMSCs were assessed by alkaline phosphatase (ALP) activity, mineralization assay and real-time RT-PCR. RESULTS: ZD-I (0.78-100 microg/ml) was non-cytotoxic. The 50% inhibitory concentration (IC50) of hMSCs was 200 microg/ml. ZD-I (0.78-50 microg/ml) stimulated the proliferation of hMSCs. ZD-I did not change ALP activity of hMSCs cultivated in osteogenic medium in the early stage (4 and 7 days), but ZD-I inhibited the mineralization of hMSCs through down-regulation of several osteogenic markers (e.g. osteocalcin, bone morphogenetic protein 2 and osteopontin) in the late stage. CONCLUSIONS: ZD-I stimulate cellular proliferation and decrease the bone mineral deposition of hMSCs. These results suggest ZD-I may play an important therapeutic role in osteoarthritic patients by improving proliferative capacity of hMSCs and inhibiting the mineralization of hMSCs.

KW - Alkaline Phosphatase

KW - Cell Differentiation

KW - Cell Proliferation

KW - Culture Media

KW - Gene Expression Regulation

KW - Humans

KW - Medicine, Chinese Traditional

KW - Mesenchymal Stem Cells

KW - Osteoblasts

KW - Plant Extracts

U2 - 10.1016/j.jep.2009.06.013

DO - 10.1016/j.jep.2009.06.013

M3 - Journal article

VL - 125

SP - 75

EP - 82

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 1

ER -