A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Lijin Zou; Yonglun Luo; Muwan Chen; Gang Wang; Ming Ding; Charlotte Christie Petersen; Ran Kang; Frederik Dagnaes-Hansen; Yuanlin Zeng; Nonghua Lv; Qing Ma; Dang Quang Svend Le; Flemming Besenbacher; Lars Bolund; Thomas G Jensen; Jørgen Kjems; William T Pu; Cody Bünger

doi:10.1038/srep02243

A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Lijin Zou
, Yonglun Luo
, Muwan Chen
, Gang Wang
, Ming Ding
, Charlotte Christie Petersen
, Ran Kang
, Frederik Dagnaes-Hansen
, Yuanlin Zeng
, Nonghua Lv
, Qing Ma
, Dang Quang Svend Le
, Flemming Besenbacher
, Lars Bolund
, Thomas G Jensen
, Jørgen Kjems
, William T Pu
, Cody Bünger

KI, OUH, Research unit of orthopaedic surgery (Odense)

Aarhus University Hospital
Aarhus University
Boston Children's Hospital
Harvard University
The First Affiliated Hospital of Nanchang University
Interdisciplinary Nanoscience Center

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.

Original language	English
Article number	2243
Journal	Scientific Reports
Volume	3
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep02243
Publication status	Published - 2013

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Zou, L., Luo, Y., Chen, M., Wang, G., Ding, M., Petersen, C. C., Kang, R., Dagnaes-Hansen, F., Zeng, Y., Lv, N., Ma, Q., Le, D. Q. S., Besenbacher, F., Bolund, L., Jensen, T. G., Kjems, J., Pu, W. T., & Bünger, C. (2013). A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds. Scientific Reports, 3, Article 2243. https://doi.org/10.1038/srep02243

@article{a1b36499d77f4a27a4a0ff584eb81aca,

title = "A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds",

abstract = "We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.",

author = "Lijin Zou and Yonglun Luo and Muwan Chen and Gang Wang and Ming Ding and Petersen, \{Charlotte Christie\} and Ran Kang and Frederik Dagnaes-Hansen and Yuanlin Zeng and Nonghua Lv and Qing Ma and Le, \{Dang Quang Svend\} and Flemming Besenbacher and Lars Bolund and Jensen, \{Thomas G\} and J{\o}rgen Kjems and Pu, \{William T\} and Cody B{\"u}nger",

year = "2013",

doi = "10.1038/srep02243",

language = "English",

volume = "3",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

AU - Zou, Lijin

AU - Luo, Yonglun

AU - Chen, Muwan

AU - Wang, Gang

AU - Ding, Ming

AU - Petersen, Charlotte Christie

AU - Kang, Ran

AU - Dagnaes-Hansen, Frederik

AU - Zeng, Yuanlin

AU - Lv, Nonghua

AU - Ma, Qing

AU - Le, Dang Quang Svend

AU - Besenbacher, Flemming

AU - Bolund, Lars

AU - Jensen, Thomas G

AU - Kjems, Jørgen

AU - Pu, William T

AU - Bünger, Cody

PY - 2013

Y1 - 2013

N2 - We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.

AB - We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.

U2 - 10.1038/srep02243

DO - 10.1038/srep02243

M3 - Journal article

C2 - 23873182

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

M1 - 2243

ER -

A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Abstract

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