TY - JOUR
T1 - A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking
AU - Aguilar, Pablo S
AU - Fröhlich, Florian
AU - Rehman, Michael
AU - Shales, Mike
AU - Ulitsky, Igor
AU - Olivera-Couto, Agustina
AU - Braberg, Hannes
AU - Shamir, Ron
AU - Walter, Peter
AU - Mann, Matthias
AU - Ejsing, Christer S
AU - Krogan, Nevan J
AU - Walther, Tobias C
PY - 2010/7/1
Y1 - 2010/7/1
N2 - The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
AB - The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
U2 - 10.1038/nsmb.1829
DO - 10.1038/nsmb.1829
M3 - Journal article
C2 - 20526336
SN - 1545-9993
VL - 17
SP - 901
EP - 908
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
IS - 7
ER -