A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Large animal models allow for collection of substantial amounts of biological material. However, the collection of larger volumes (>100 ml) of blood from pigs can be a challenge: (i) the peripheral veins are not suitable for collection of high numbers of standard blood tubes as the veins tend to collapse; and (ii) the alternative option of cannulating deeper veins mandates surgical exposure of the vessels and often the need for heparinization, which is undesirable for some blood analysis. During an immunization trial in 40 pigs, we assessed the femoral and saphenous arteries as practical vessels for collecting 250 ml of blood from each pig in standard collection tubes without heparinization. Blood collected from the saphenous artery by a standard butterfly needle proved particularly useful and 250 ml blood could be collected successfully in 24 of 25 pigs by this approach.

Original languageEnglish
JournalLaboratory Animals
Pages (from-to)236772221133431
ISSN0023-6772
DOIs
Publication statusE-pub ahead of print - 11. Nov 2022

Fingerprint

Dive into the research topics of 'A method for collecting high numbers of blood samples in standard vacuum tubes from non-heparinized pigs'. Together they form a unique fingerprint.

Cite this