A method for analysis of lipid vesicle domain structure from confocal image data

Peter Rasmussen Husen, Matthias Fidorra, Steffen Hartel, Luis Bagatolli, John Hjort Ipsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Quantitative characterization of the lateral structure of curved membranes based
on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for extraction of the fluorophore distribution on a spherical lipid vesicle from confocal imaging stacks. The technique involves projection of volumetric image data onto a triangulated surface mesh representation of the membrane, correction of photoselection effects and global motion of the vesicle during image acquisition and segmentation of the surface into domains using histograms. The analysis allows for investigation of the morphology and size distribution of domains on the surface.
Original languageEnglish
JournalEuropean Biophysics Journal
Volume41
Issue number2
Pages (from-to)161-175
ISSN0175-7571
DOIs
Publication statusPublished - 2012

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