TY - JOUR
T1 - A Highly Photostable Hyperbranched Polyglycerol-Based NIR Fluorescence Nanoplatform for Mitochondria-Specific Cell Imaging
AU - Dong, Chunhong
AU - Liu, Zhongyun
AU - Liu, Junqing
AU - Wu, Changzhu
AU - Neumann, Falko
AU - Wang, Hanjie
AU - Schäfer-Korting, Monika
AU - Kleuser, Burkhard
AU - Chang, Jin
AU - Li, Wenzhong
AU - Ma, Nan
AU - Haag, Rainer
PY - 2016/6/2
Y1 - 2016/6/2
N2 - Considering the critical role of mitochondria in the life and death of cells, non-invasive long-term tracking of mitochondria has attracted considerable interest. However, a high-performance mitochondria-specific labeling probe with high photostability is still lacking. Herein a highly photostable hyperbranched polyglycerol (hPG)-based near-infrared (NIR) quantum dots (QDs) nanoplatform is reported for mitochondria-specific cell imaging. Comprising NIR Zn-Cu-In-S/ZnS QDs as extremely photostable fluorescent labels and alkyl chain (C
12)/triphenylphosphonium (TPP)-functionalized hPG derivatives as protective shell, the tailored QDs@hPG-C
12/TPP nanoprobe with a hydrodynamic diameter of about 65 nm exhibits NIR fluorescence, excellent biocompatibility, good stability, and mitochondria-targeted ability. Cell uptake experiments demonstrate that QDs@hPG-C
12/TPP displays a significantly enhanced uptake in HeLa cells compared to nontargeted QDs@hPG-C
12. Further co-localization study indicates that the probe selectively targets mitochondria. Importantly, compared with commercial deep-red mitochondria dyes, QDs@hPG-C
12/TPP possesses superior photostability under continuous laser irradiation, indicating great potential for long-term mitochondria labeling and tracking. Moreover, drug-loaded QDs@hPG-C
12/TPP display an enhanced tumor cell killing efficacy compared to nontargeted drugs. This work could open the door to the construction of organelle-targeted multifunctional nanoplatforms for precise diagnosis and high-efficient tumor therapy.
AB - Considering the critical role of mitochondria in the life and death of cells, non-invasive long-term tracking of mitochondria has attracted considerable interest. However, a high-performance mitochondria-specific labeling probe with high photostability is still lacking. Herein a highly photostable hyperbranched polyglycerol (hPG)-based near-infrared (NIR) quantum dots (QDs) nanoplatform is reported for mitochondria-specific cell imaging. Comprising NIR Zn-Cu-In-S/ZnS QDs as extremely photostable fluorescent labels and alkyl chain (C
12)/triphenylphosphonium (TPP)-functionalized hPG derivatives as protective shell, the tailored QDs@hPG-C
12/TPP nanoprobe with a hydrodynamic diameter of about 65 nm exhibits NIR fluorescence, excellent biocompatibility, good stability, and mitochondria-targeted ability. Cell uptake experiments demonstrate that QDs@hPG-C
12/TPP displays a significantly enhanced uptake in HeLa cells compared to nontargeted QDs@hPG-C
12. Further co-localization study indicates that the probe selectively targets mitochondria. Importantly, compared with commercial deep-red mitochondria dyes, QDs@hPG-C
12/TPP possesses superior photostability under continuous laser irradiation, indicating great potential for long-term mitochondria labeling and tracking. Moreover, drug-loaded QDs@hPG-C
12/TPP display an enhanced tumor cell killing efficacy compared to nontargeted drugs. This work could open the door to the construction of organelle-targeted multifunctional nanoplatforms for precise diagnosis and high-efficient tumor therapy.
KW - NIR ZCIS/ZnS QDs
KW - hyperbranched polyglycerol
KW - mitochondria targeting
KW - photostability
KW - tumor theranostics
U2 - 10.1002/adhm.201600212
DO - 10.1002/adhm.201600212
M3 - Journal article
SN - 2192-2640
VL - 5
SP - 2214
EP - 2226
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 17
ER -