A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia

Sukru Palanduz, Nedime Serakinci, Kivanc Cefle, Melih Aktan, Gulcin Tutkan, Sukru Ozturk, Gokay Bozkurt, Guncag Dincol, Yuksel Pekcelen, Jørn Koch

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Telomeric sequences, located at the very end of the chromosomes, compensate for the chromosomal shortening as it happens after each round of cell division. Telomeric sequences influence the progress of cellular senescence and cancer progression. It has been reported that telomeres are shortened in acute leukemias where the cell turnover is high. B-cell chronic lymphocytic leukemia (CLL) is a particularly interesting haematological malignancy in regard to telomere dynamics because most of the malignant cells in CLL are mitotically inactive. In this study, we analysed the telomere length in patients with B-cell CLL in a comparison with the control group by using ddPRINS technique. Twenty patients with CLL and four healthy donors as a control group were included. We found short telomeres and no detectable telomeric repeats at the sites of chromosome fusion. We hypothesise that the telomeric erosion in CLL may reflect the dominance of malignant cells with an abnormally long life span. These cells may have encountered many antigenic stimulants in the past and hence underwent multiple clonal expansions. Our findings imply that shortened telomeres in CLL may be reflecting the "history" of the disease and serve as an independent prognostic factor.

Original languageEnglish
JournalEuropean Journal of Medical Genetics
Volume49
Pages (from-to)63-69
Number of pages6
ISSN1769-7212
Publication statusPublished - 1. Jan 2006

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Telomere
B-Cell Chronic Lymphocytic Leukemia
Telomere Shortening
Control Groups
Cell Aging
Hematologic Neoplasms
Leukemia
History
Neoplasms

Cite this

Palanduz, S., Serakinci, N., Cefle, K., Aktan, M., Tutkan, G., Ozturk, S., ... Koch, J. (2006). A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia. European Journal of Medical Genetics, 49, 63-69.
Palanduz, Sukru ; Serakinci, Nedime ; Cefle, Kivanc ; Aktan, Melih ; Tutkan, Gulcin ; Ozturk, Sukru ; Bozkurt, Gokay ; Dincol, Guncag ; Pekcelen, Yuksel ; Koch, Jørn. / A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia. In: European Journal of Medical Genetics. 2006 ; Vol. 49. pp. 63-69.
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Palanduz, S, Serakinci, N, Cefle, K, Aktan, M, Tutkan, G, Ozturk, S, Bozkurt, G, Dincol, G, Pekcelen, Y & Koch, J 2006, 'A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia', European Journal of Medical Genetics, vol. 49, pp. 63-69.

A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia. / Palanduz, Sukru; Serakinci, Nedime; Cefle, Kivanc; Aktan, Melih; Tutkan, Gulcin; Ozturk, Sukru; Bozkurt, Gokay; Dincol, Guncag; Pekcelen, Yuksel; Koch, Jørn.

In: European Journal of Medical Genetics, Vol. 49, 01.01.2006, p. 63-69.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia

AU - Palanduz, Sukru

AU - Serakinci, Nedime

AU - Cefle, Kivanc

AU - Aktan, Melih

AU - Tutkan, Gulcin

AU - Ozturk, Sukru

AU - Bozkurt, Gokay

AU - Dincol, Guncag

AU - Pekcelen, Yuksel

AU - Koch, Jørn

N1 - Paper id:: PMID: 16473311 [PubMed - indexed for MEDLINE]

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Telomeric sequences, located at the very end of the chromosomes, compensate for the chromosomal shortening as it happens after each round of cell division. Telomeric sequences influence the progress of cellular senescence and cancer progression. It has been reported that telomeres are shortened in acute leukemias where the cell turnover is high. B-cell chronic lymphocytic leukemia (CLL) is a particularly interesting haematological malignancy in regard to telomere dynamics because most of the malignant cells in CLL are mitotically inactive. In this study, we analysed the telomere length in patients with B-cell CLL in a comparison with the control group by using ddPRINS technique. Twenty patients with CLL and four healthy donors as a control group were included. We found short telomeres and no detectable telomeric repeats at the sites of chromosome fusion. We hypothesise that the telomeric erosion in CLL may reflect the dominance of malignant cells with an abnormally long life span. These cells may have encountered many antigenic stimulants in the past and hence underwent multiple clonal expansions. Our findings imply that shortened telomeres in CLL may be reflecting the "history" of the disease and serve as an independent prognostic factor.

AB - Telomeric sequences, located at the very end of the chromosomes, compensate for the chromosomal shortening as it happens after each round of cell division. Telomeric sequences influence the progress of cellular senescence and cancer progression. It has been reported that telomeres are shortened in acute leukemias where the cell turnover is high. B-cell chronic lymphocytic leukemia (CLL) is a particularly interesting haematological malignancy in regard to telomere dynamics because most of the malignant cells in CLL are mitotically inactive. In this study, we analysed the telomere length in patients with B-cell CLL in a comparison with the control group by using ddPRINS technique. Twenty patients with CLL and four healthy donors as a control group were included. We found short telomeres and no detectable telomeric repeats at the sites of chromosome fusion. We hypothesise that the telomeric erosion in CLL may reflect the dominance of malignant cells with an abnormally long life span. These cells may have encountered many antigenic stimulants in the past and hence underwent multiple clonal expansions. Our findings imply that shortened telomeres in CLL may be reflecting the "history" of the disease and serve as an independent prognostic factor.

M3 - Journal article

VL - 49

SP - 63

EP - 69

JO - European Journal of Medical Genetics

JF - European Journal of Medical Genetics

SN - 1769-7212

ER -

Palanduz S, Serakinci N, Cefle K, Aktan M, Tutkan G, Ozturk S et al. A different approach to telomere analysis with ddPRINS in chronic lymphocytic leukemia. European Journal of Medical Genetics. 2006 Jan 1;49:63-69.