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2019
91 Downloads (Pure)

A self-assembled, modular nucleic acid-based nanoscaffold for multivalent theranostic medicine

Andersen, V. L., Vinther, M., Kumar, R., Ries, A., Wengel, J., Nielsen, J. S. & Kjems, J., 2019, In : Theranostics. 9, 9, p. 2662-2677

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Nucleic Acids
Medicine
Precision Medicine
Confocal Microscopy
Hepatocytes
159 Downloads (Pure)

Fibrin-hyaluronic acid hydrogel-based delivery of antisense oligonucleotides for ADAMTS5 inhibition in co-delivered and resident joint cells in osteoarthritis

Garcia, J. P., Stein, J., Cai, Y., Riemers, F., Wexselblatt, E., Wengel, J., Tryfonidou, M., Yayon, A., Howard, K. A. & Creemers, L. B., 28. Jan 2019, In : Journal of Controlled Release. 294, p. 247-258

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Hydrogel
Hyaluronic Acid
Chondrocytes
Joints
Disintegrins

Improved RE31 Analogues Containing Modified Nucleic Acid Monomers: Thermodynamic, Structural, and Biological Effects

Kotkowiak, W., Wengel, J., Scotton, C. J. & Pasternak, A., 14. Mar 2019, In : Journal of Medicinal Chemistry. 62, 5, p. 2499-2507

Research output: Contribution to journalJournal articleResearchpeer-review

Nucleic Acids
Nucleotide Aptamers
RNA
Freezing
Serum
94 Downloads (Pure)

microRNA-155 inhibition restores Fibroblast Growth Factor 7 expression in diabetic skin and decreases wound inflammation

Moura, J., Sørensen, A., Leal, E. C., Svendsen, R., Carvalho, L., Willemoes, R. J., Jørgensen, P. T., Jenssen, H., Wengel, J., Dalgaard, L. T. & Carvalho, E., 9. Apr 2019, In : Scientific Reports. 9, 11 p., 5836.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fibroblast Growth Factor 7
MicroRNAs
Skin
Wounds and Injuries
Diabetic Foot
41 Downloads (Pure)

Molecular determinants for agonist recognition and discrimination in P2X2 receptors

Gasparri, F., Wengel, J., Grutter, T. & Pless, S. A., 1. Jul 2019, In : Journal of General Physiology. 151, 7, p. 898-911 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Ligands
Site-Directed Mutagenesis
Amides
Cations
Esters

Novel assemblies based on oligonucleotides containing intercalating nucleic acid monomers

Abdelrahman, A., Gouda, A. S., Jørgensen, P. T. & Wengel, J., 1. Nov 2019, In : Nucleosides, Nucleotides and Nucleic Acids.

Research output: Contribution to journalJournal articleResearchpeer-review

Oligonucleotides
Nucleic Acids
Monomers
Molecular modeling
Fluorescence spectroscopy
83 Downloads (Pure)

Oligonucleotide binding to non-B-DNA in MYC

Umek, T., Sollander, K., Bergquist, H., Wengel, J., Lundin, K. E., Edvard Smith, C. I. & Zain, R., 12. Mar 2019, In : Molecules. 24, 5, 11 p., 1000.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
oligonucleotides
Oligonucleotides
genes
strands
Genes
35 Downloads (Pure)

Synthetic, structural, and anticancer activity evaluation studies on novel pyrazolylnucleosides

Yadav, Y., Sharma, D., Kaushik, K., Kumar, V., Jha, A., Prasad, A. K., Len, C., Malhotra, S. V., Wengel, J. & Parmar, V. S., 30. Oct 2019, In : Molecules. 24, 21, 16 p., 3922.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
cultured cells
Cells
Cell Line
evaluation
cancer
103 Downloads (Pure)

The ability of locked nucleic acid oligonucleotides to pre-structure the double helix: A molecular simulation and binding study

Xu, Y., Gissberg, O., Pabon-Martinez, Y. V., Wengel, J., Lundin, K. E., Smith, C. I. E., Zain, R., Nilsson, L. & Villa, A., 2019, In : PLOS ONE. 14, 2, 14 p., e0211651.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
oligonucleotides
Oligonucleotides
nucleic acids
DNA
molecular dynamics
2018
232 Downloads (Pure)

Hydrogel-Assisted Antisense LNA Gapmer Delivery for In Situ Gene Silencing in Spinal Cord Injury

Moreno, P. M. D., Ferreira, A. R., Salvador, D., Rodrigues, M. T., Torrado, M., Carvalho, E. D., Tedebark, U., Sousa, M. M., Amaral, I. F., Wengel, J. & Pêgo, A. P., 2018, In : Molecular Therapy - Nucleic Acids. 11, p. 393-406

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Hydrogel
Spinal Ganglia
Nucleic Acids
Cell Survival
Down-Regulation
102 Downloads (Pure)

MicroRNA therapeutics: design of single-stranded miR-216b mimics to target KRAS in pancreatic cancer cells

Ferino, A., Miglietta, G., Picco, R., Vogel, S., Wengel, J. & Xodo, L. E., 2018, In : RNA Biology. 15, 10, p. 1273-1285

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
MicroRNAs
Pancreatic Neoplasms
Cell-Penetrating Peptides
Nucleic Acids
Down-Regulation
77 Downloads (Pure)

Modification of oligodeoxynucleotides by on-column Suzuki cross-coupling reactions

Ejlersen, M., Lou, C., Sanghvi, Y. S., Tor, Y. & Wengel, J., 12. Jul 2018, In : Chemical Communications. 54, 57, p. 8003-8006

Research output: Contribution to journalLetterResearchpeer-review

Open Access
File
Oligonucleotides
Oligodeoxyribonucleotides
Boronic Acids
Monomers
Acids
98 Downloads (Pure)

Novel conformationally constrained 2'-C-methylribonucleosides: synthesis and incorporation into oligonucleotides

Vejlegaard, K., Wegeberg, C., McKee, V. & Wengel, J., 2018, In : Organic & Biomolecular Chemistry. 16, 8, p. 1312-1321

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Oligonucleotides
Bearings (structural)
Derivatives
Ribonucleosides
Complementary RNA
74 Downloads (Pure)

Palmitoylated phosphodiester gapmer designs with albumin binding capacity and maintained in vitro gene silencing activity

Cai, Y., Makarova, A-M., Wengel, J. & Howard, K. A., 2018, In : Journal of Gene Medicine. 20, 7-8, p. 1-6

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Caco-2 Cells
Albumins
erbB-1 Genes
Flow Cytometry
Fatty Acids
104 Downloads (Pure)

Synergy of Two Highly Specific Biomolecular Recognition Events: Aligning an AT-Hook Peptide in DNA Minor Grooves via Covalent Conjugation to 2'-Amino-LNA

Ejlersen, M., Christensen, N. J., Sørensen, K. K., Jensen, K. J., Wengel, J. & Lou, C., 2018, In : Bioconjugate Chemistry. 29, 4, p. 1025–1029

Research output: Contribution to journalLetterResearchpeer-review

Open Access
File
AT-Hook Motifs
Hooks
Peptides
DNA
Oligonucleotides
110 Downloads (Pure)

Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides

Lima, J. F., Carvalho, J., Pinto-Ribeiro, I., Almeida, C., Wengel, J., Cerqueira, L., Figueiredo, C., Oliveira, C. & Azevedo, N. F., 7. Jun 2018, In : BMC Molecular Biology. 19, 13 p., 6.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
MicroRNAs
Cell Movement
Culture Media
Up-Regulation
94 Downloads (Pure)

Thermodynamic, Anticoagulant, and Antiproliferative Properties of Thrombin Binding Aptamer Containing Novel UNA Derivative

Kotkowiak, W., Lisowiec-Wachnicka, J., Grynda, J., Kierzek, R., Wengel, J. & Pasternak, A., 2018, In : Molecular Therapy - Nucleic Acids. 10, p. 304-316

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Nucleic Acids
Fibrinolysis
HeLa Cells
Cell Movement
Cell Proliferation
90 Downloads (Pure)

Unlocked nucleic acid modified primer-based enzymatic polymerization assay: Towards allele-specific genotype detection of human platelet antigens

Le, B. T., Hughes, Q., Rakesh, S., Baker, R., Jørgensen, P. T., Wengel, J. & Veedu, R. N., 2018, In : RSC Advances. 8, 57, p. 32770-32774

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Human Platelet Antigens
Nucleic acids
Antigens
Nucleotides
Platelets
2017

3'-Pyrene-modified unlocked nucleic acids: synthesis, fluorescence properties and a surprising stabilization effect on duplexes and triplexes

Ejlersen, M., Langkjær, N. & Wengel, J., 2017, In : Organic & Biomolecular Chemistry. 15, 9, p. 2073-2085

Research output: Contribution to journalJournal articleResearchpeer-review

Nucleic Acids
Oligonucleotides
Stabilization
Fluorescence
DNA
137 Downloads (Pure)

A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis

Cai, Y., López-Ruiz, E., Wengel, J., Creemers, L. B. & Howard, K. A., 2017, In : Journal of controlled release : official journal of the Controlled Release Society. 253, p. 153-159

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Hydrogel
Hyaluronic Acid
Chondrocytes
Patient Participation
Schiff Bases
144 Downloads (Pure)

An Albumin-Oligonucleotide Assembly for Potential Combinatorial Drug Delivery and Half-Life Extension Applications

Kuhlmann, M., Hamming, J. B. R., Voldum, A., Tsakiridou, G., Larsen, M. T., Schmøkel, J. S., Sohn, E., Bienk, K., Schaffert, D., Sørensen, E. S., Wengel, J., Dupont, D. M. & Howard, K. A., 2017, In : Molecular Therapy - Nucleic Acids. 9, p. 284-293

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Life Expectancy
Half-Life
Albumins
Factor IXa
Pharmaceutical Preparations
300 Downloads (Pure)

CTG repeat-targeting oligonucleotides for down-regulating Huntingtin expression

Zaghloul, E. M., Gissberg, O., Moreno, P. M. D., Siggens, L., Hällbrink, M., Jørgensen, A. S., Ekwall, K., Zain, R., Wengel, J., Lundin, K. E. & Smith, C. I. E., 2017, In : Nucleic Acids Research. 45, 9, p. 5153-5169

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Huntington Disease
Trinucleotide Repeat Expansion
Gene Knockdown Techniques
Messenger RNA
Proteins
131 Downloads (Pure)

Development of an Efficient G-Quadruplex-Stabilised Thrombin-Binding Aptamer Containing a Three-Carbon Spacer Molecule

Aaldering, L. J., Poongavanam, V., Langkjær, N., Murugan, N. A., Jørgensen, P. T., Wengel, J. & Veedu, R. N., 2017, In : ChemBioChem. 18, 8, p. 755–763

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Carbon
Molecules
Thrombin
Nucleic Acids
Circular dichroism spectroscopy
107 Downloads (Pure)

Fatty Acid-Modified Gapmer Antisense Oligonucleotide and Serum Albumin Constructs for Pharmacokinetic Modulation

Hvam, M. L., Cai, Y., Dagnæs-Hansen, F., Nielsen, J. S., Wengel, J., Kjems, J. & Howard, K. A., 2017, In : Molecular Therapy. 25, 7, p. 1710-1717

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fatty Acids
Pharmacokinetics
Albumins
Palmitic Acid
Myristic Acid
115 Downloads (Pure)

Folding Topology of a Short Coiled-Coil Peptide Structure Templated by an Oligonucleotide Triplex

Lou, C., Christensen, N. J., Martos-Maldonado, M. C., Midtgaard, S. R., Ejlersen, M., Thulstrup, P. W., Sørensen, K. K., Jensen, K. J. & Wengel, J., 2017, In : Chemistry: A European Journal. 23, 39, p. 9297–9305

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Oligonucleotides
Peptides
Topology
Circular dichroism spectroscopy
Denaturation
181 Downloads (Pure)

LNA effects on DNA binding and conformation: from single strand to duplex and triplex structures

Pabon-Martinez, Y. V., Xu, Y., Villa, A., Lundin, K. E., Geny, S., Nguyen, C-H., Pedersen, E. B., Jørgensen, P. T., Wengel, J., Nilsson, L., Smith, C. I. E. & Zain, R., 2017, In : Scientific Reports. 7, 1, 16 p., 11043.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
DNA
Electrophoretic Mobility Shift Assay
Molecular Dynamics Simulation
Nucleic Acids
triplex DNA
215 Downloads (Pure)

Oligodeoxynucleotides containing 2'-amino-LNA nucleotides as constrained morpholino phosphoramidate and phosphorodiamidate monomers

Kristensen, K. V., Paul, S., Kosbar, T., Wengel, J. & Caruthers, M. H., 2017, In : Bioorganic & Medicinal Chemistry Letters. 27, 14, p. 3173–3176

Research output: Contribution to journalLetterResearchpeer-review

Open Access
File
Morpholinos
Oligodeoxyribonucleotides
Nucleotides
Monomers
Complementary RNA

Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Lou, C., Samuelsen, S. V., Christensen, N. J., Vester, B. & Wengel, J., 2017, In : Bioconjugate Chemistry. 28, 4, p. 1214-1220

Research output: Contribution to journalJournal articleResearchpeer-review

Complementary RNA
Oligonucleotides
Nucleotides
RNA
DNA
234 Downloads (Pure)

Role of Pseudoisocytidine Tautomerization in Triplex-Forming Oligonucleotides: In Silico and in Vitro Studies

Hartono, Y. D., Pabon-Martinez, Y. V., Uyar, A., Wengel, J., Lundin, K. E., Zain, R., Smith, C. I. E., Nilsson, L. & Villa, A., 2017, In : ACS Omega. 2, 5, p. 2165-2177 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Cytidine
Oligonucleotides
Thymine
DNA sequences
Nucleic acids

Site-selective conjugation of an anticoagulant aptamer to recombinant albumins and maintenance of neonatal Fc receptor binding

Schmøkel, J., Voldum, A., Tsakiridou, G., Kuhlmann, M., Cameron, J., Sørensen, E. S., Wengel, J. & Howard, K. A., 2017, In : Nanotechnology. 28, 20, 9 p., 204004.

Research output: Contribution to journalJournal articleResearchpeer-review

Pharmacokinetics
Nucleic acids
High performance liquid chromatography
Cyclohexane
Electrophoresis
146 Downloads (Pure)

Synthesis and Excellent Duplex Stability of Oligonucleotides Containing 2'-Amino-LNA Functionalized with Galactose Units

Kumar, R., Ries, A. & Wengel, J., 2017, In : Molecules. 22, 5, 13 p., 852.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
galactose
oligonucleotides
Galactose
Oligonucleotides
complementary DNA
2015

Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH)

Azevedo, A. S., Almeida, C., Pereira, B., Madureira, P., Wengel, J. & Azevedo, N. F., 2015, In : Biochemical Engineering Journal. 104, 15 December, p. 64-73 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Biofilms
Fluorescence
RNA
Oligonucleotide Probes
Catheters
132 Downloads (Pure)

Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori

Santos, R. S., Dakwar, G. R., Xiong, R., Forier, K., Remaut, K., Stremersch, S., Guimarães, N., Fontenete, S., Wengel, J., Leite, M., Figueiredo, C., De Smedt, S. C., Braeckmans, K. & Azevedo, N. F., 10. Dec 2015, In : Molecular Therapy - Nucleic Acids. 4, p. e269 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Mucus
Helicobacter pylori
RNA
Nucleic Acids
Population

Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids

Fontenete, S., Joana, B., Pedro, M., Céu, F., Wengel, J. & Filipe, A. N., May 2015, In : Applied Microbiology and Biotechnology. 99, 9, p. 3961-3969

Research output: Contribution to journalJournal articleResearchpeer-review

Nucleic Acids
Fluorescent Dyes
RNA
Peptide Nucleic Acids
Helicobacter pylori
136 Downloads (Pure)

Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer

Opazo, F., Eiden, L., Hansen, L., Rohrbach, F., Wengel, J., Kjems, J. & Mayer, G., 2015, In : Molecular Therapy - Nucleic Acids. 4, 10 p., e251.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Nucleotide Aptamers
Quantum Dots
Equipment and Supplies
Clathrin
Endocytosis
167 Downloads (Pure)

Oligonucleotides containing a piperazino-modified 2'-amino-LNA monomer exhibit very high duplex stability and remarkable nuclease resistance

Lou, C., Vester, B. & Wengel, J., 9. Feb 2015, In : Chemical Communications. 51, 19, p. 4024-4027

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Oligonucleotides
Monomers
Complementary RNA
RNA
Nucleosides
191 Downloads (Pure)

Selection of LNA-containing DNA aptamers against recombinant human CD73

Elle, I. C., Karlsen, K. K., Terp, M. G., Larsen, N., Nielsen, R., Derbyshire, N., Mandrup, S., Ditzel, H. J. & Wengel, J., 21. Apr 2015, In : Molecular BioSystems. 11, 5, p. 1260-1270

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Nucleotide Aptamers
Surface Plasmon Resonance
Computational Biology
Libraries
Cluster Analysis

Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition

Edwards, S. L., Poongavanam, V., R Kanwar, J., Roy, K., M. Hillman, K., Prasad, N., Leth-Larsen, R., Petersen, M., Marusic, M., Plavec, J., Wengel, J. & Veedu, R. N., 7. May 2015, In : Chemical Communications. 51, 46, p. 9499-9502

Research output: Contribution to journalLetterResearchpeer-review

Oligonucleotides
Nucleic acids
Vascular Endothelial Growth Factor A
Nucleotide Aptamers
Vascular Endothelial Growth Factor Receptor
317 Downloads (Pure)

Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes

Fontenete, S., Leite, M., Guimarães, N., Madureira, P., Ferreira, R. M., Figueiredo, C., Wengel, J. & Azevedo, N. F., 2015, In : PLOS ONE. 10, 4, p. e0125494 22 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Nucleic Acid Probes
pylorus
gastric mucosa
Pylorus
fluorescence in situ hybridization

Watson-Crick hydrogen bonding of unlocked nucleic acids

Langkjær, N., Wengel, J. & Pasternak, A., 15. Nov 2015, In : Bioorganic & Medicinal Chemistry Letters. 25, 22, p. 5064-6 3 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Hydrogen Bonding
Oligonucleotides
Nucleic Acids
Hydrogen bonds
Hypoxanthine