2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Santhosh T. Kumar, Jesper Wengel, Patrick J. Hrdlicka

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Precise positioning of intercalators furnishes a SNP‐detection tool. The conformationally locked 2‐oxo‐5‐azabicyclo‐[2.2.1]heptane skeleton of 2′‐amino‐α‐L‐LNA monomer X directs the N2′‐linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single‐nucleotide mismatches in DNA/RNA targets by excimer signal formation.
Original languageEnglish
JournalChemBioChem
Volume8
Issue number10
Pages (from-to)1122-1125
ISSN1439-4227
DOIs
Publication statusPublished - 2007

Fingerprint Dive into the research topics of '2'-N-(Pyren-1-yl)acetyl-2'-Amino-a-L-LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets'. Together they form a unique fingerprint.

Cite this