Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis

Lars Koch Hansen, Dorte Larsen, Veeranjaneyulu Sadda, Gorm Nielsen, Lone Klinge, Ove B. Schaffalitzky de Muckadell, Torben Knudsen, Jens Kjeldsen, Ralf Köhler

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

INTRODUCTION: T-cell KV1.3 and KCa3.1 channels have been proposed to be important effector proteins during T-cell activation and also in autoimmune disease by controlling T-cell motility, cytokine production, and proliferation. The role of KV1.3 channels in ulcerative colitis (UC) has not been defined yet. In the present study we hypothesized that T-cell KV1.3 and KCa3.1 expression may serve as novel molecular marker for T-cell infiltration, inflammatory activity, and
possibly disease progression.
AIMS & METHODS: Biopsies were obtained from the rectal mucosa in seven UC patients with clinical and endoscopic active disease and seven non-UC patients. Active inflammation in the rectal mucosa in patients with UC was verified histologically. Expression of KV1.3 and KCa3.1 genes and of INFγ as a classical marker of inflammation was investigated in mRNA-extracts from biopsies by quantitative RT-PCR (qPCR).
RESULTS: Expression of KV1.3 was significantly ~7-foldly increased in UC patients compared to the very low background expression in controls (P<0.05). KCa3.1 gene expression tended also to be 2-foldly increased in UC patients
compared to the basal physiological expression in intestinal epithelium of controls (P=0.1). Expression of INFγ as classical marker of inflammation was also ~10-fold increased in UC patients compared to the very low background
expression levels found in non-UC controls (P<0.05).
CONCLUSION: Expression of KV1.3 and, possibly, KCa3.1 channels are increased in a disease related fashion in UC patients and may point to an increased infiltration and activity of effector T-cells and effector memory T-cells in UC.
These markers could be predictive of progression and for adjusting individual immune suppressive treatments.
Original languageEnglish
Publication date22. Oct 2011
Number of pages1
Publication statusPublished - 22. Oct 2011
EventUnited European Gastroenterology Week (UEGW) 2011 - Stockholm, Sweden
Duration: 22. Oct 201126. Oct 2011

Conference

ConferenceUnited European Gastroenterology Week (UEGW) 2011
CountrySweden
CityStockholm
Period22/10/201126/10/2011

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Ulcerative Colitis
Colitis
Mucous Membrane
Intestinal Mucosa
Cell Movement
Polymerase Chain Reaction
Messenger RNA
Proteins

Cite this

Hansen, L. K., Larsen, D., Sadda, V., Nielsen, G., Klinge, L., Schaffalitzky de Muckadell, O. B., ... Köhler, R. (2011). Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis. Poster session presented at United European Gastroenterology Week (UEGW) 2011, Stockholm, Sweden.
Hansen, Lars Koch ; Larsen, Dorte ; Sadda, Veeranjaneyulu ; Nielsen, Gorm ; Klinge, Lone ; Schaffalitzky de Muckadell, Ove B. ; Knudsen, Torben ; Kjeldsen, Jens ; Köhler, Ralf. / Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis. Poster session presented at United European Gastroenterology Week (UEGW) 2011, Stockholm, Sweden.1 p.
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title = "Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis",
abstract = "INTRODUCTION: T-cell KV1.3 and KCa3.1 channels have been proposed to be important effector proteins during T-cell activation and also in autoimmune disease by controlling T-cell motility, cytokine production, and proliferation. The role of KV1.3 channels in ulcerative colitis (UC) has not been defined yet. In the present study we hypothesized that T-cell KV1.3 and KCa3.1 expression may serve as novel molecular marker for T-cell infiltration, inflammatory activity, andpossibly disease progression.AIMS & METHODS: Biopsies were obtained from the rectal mucosa in seven UC patients with clinical and endoscopic active disease and seven non-UC patients. Active inflammation in the rectal mucosa in patients with UC was verified histologically. Expression of KV1.3 and KCa3.1 genes and of INFγ as a classical marker of inflammation was investigated in mRNA-extracts from biopsies by quantitative RT-PCR (qPCR).RESULTS: Expression of KV1.3 was significantly ~7-foldly increased in UC patients compared to the very low background expression in controls (P<0.05). KCa3.1 gene expression tended also to be 2-foldly increased in UC patientscompared to the basal physiological expression in intestinal epithelium of controls (P=0.1). Expression of INFγ as classical marker of inflammation was also ~10-fold increased in UC patients compared to the very low backgroundexpression levels found in non-UC controls (P<0.05).CONCLUSION: Expression of KV1.3 and, possibly, KCa3.1 channels are increased in a disease related fashion in UC patients and may point to an increased infiltration and activity of effector T-cells and effector memory T-cells in UC.These markers could be predictive of progression and for adjusting individual immune suppressive treatments.",
keywords = "colitis ulcerosa, translationel forskning",
author = "Hansen, {Lars Koch} and Dorte Larsen and Veeranjaneyulu Sadda and Gorm Nielsen and Lone Klinge and {Schaffalitzky de Muckadell}, {Ove B.} and Torben Knudsen and Jens Kjeldsen and Ralf K{\"o}hler",
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Hansen, LK, Larsen, D, Sadda, V, Nielsen, G, Klinge, L, Schaffalitzky de Muckadell, OB, Knudsen, T, Kjeldsen, J & Köhler, R 2011, 'Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis', United European Gastroenterology Week (UEGW) 2011, Stockholm, Sweden, 22/10/2011 - 26/10/2011.

Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis. / Hansen, Lars Koch; Larsen, Dorte; Sadda, Veeranjaneyulu; Nielsen, Gorm; Klinge, Lone; Schaffalitzky de Muckadell, Ove B. ; Knudsen, Torben; Kjeldsen, Jens; Köhler, Ralf.

2011. Poster session presented at United European Gastroenterology Week (UEGW) 2011, Stockholm, Sweden.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis

AU - Hansen, Lars Koch

AU - Larsen, Dorte

AU - Sadda, Veeranjaneyulu

AU - Nielsen, Gorm

AU - Klinge, Lone

AU - Schaffalitzky de Muckadell, Ove B.

AU - Knudsen, Torben

AU - Kjeldsen, Jens

AU - Köhler, Ralf

PY - 2011/10/22

Y1 - 2011/10/22

N2 - INTRODUCTION: T-cell KV1.3 and KCa3.1 channels have been proposed to be important effector proteins during T-cell activation and also in autoimmune disease by controlling T-cell motility, cytokine production, and proliferation. The role of KV1.3 channels in ulcerative colitis (UC) has not been defined yet. In the present study we hypothesized that T-cell KV1.3 and KCa3.1 expression may serve as novel molecular marker for T-cell infiltration, inflammatory activity, andpossibly disease progression.AIMS & METHODS: Biopsies were obtained from the rectal mucosa in seven UC patients with clinical and endoscopic active disease and seven non-UC patients. Active inflammation in the rectal mucosa in patients with UC was verified histologically. Expression of KV1.3 and KCa3.1 genes and of INFγ as a classical marker of inflammation was investigated in mRNA-extracts from biopsies by quantitative RT-PCR (qPCR).RESULTS: Expression of KV1.3 was significantly ~7-foldly increased in UC patients compared to the very low background expression in controls (P<0.05). KCa3.1 gene expression tended also to be 2-foldly increased in UC patientscompared to the basal physiological expression in intestinal epithelium of controls (P=0.1). Expression of INFγ as classical marker of inflammation was also ~10-fold increased in UC patients compared to the very low backgroundexpression levels found in non-UC controls (P<0.05).CONCLUSION: Expression of KV1.3 and, possibly, KCa3.1 channels are increased in a disease related fashion in UC patients and may point to an increased infiltration and activity of effector T-cells and effector memory T-cells in UC.These markers could be predictive of progression and for adjusting individual immune suppressive treatments.

AB - INTRODUCTION: T-cell KV1.3 and KCa3.1 channels have been proposed to be important effector proteins during T-cell activation and also in autoimmune disease by controlling T-cell motility, cytokine production, and proliferation. The role of KV1.3 channels in ulcerative colitis (UC) has not been defined yet. In the present study we hypothesized that T-cell KV1.3 and KCa3.1 expression may serve as novel molecular marker for T-cell infiltration, inflammatory activity, andpossibly disease progression.AIMS & METHODS: Biopsies were obtained from the rectal mucosa in seven UC patients with clinical and endoscopic active disease and seven non-UC patients. Active inflammation in the rectal mucosa in patients with UC was verified histologically. Expression of KV1.3 and KCa3.1 genes and of INFγ as a classical marker of inflammation was investigated in mRNA-extracts from biopsies by quantitative RT-PCR (qPCR).RESULTS: Expression of KV1.3 was significantly ~7-foldly increased in UC patients compared to the very low background expression in controls (P<0.05). KCa3.1 gene expression tended also to be 2-foldly increased in UC patientscompared to the basal physiological expression in intestinal epithelium of controls (P=0.1). Expression of INFγ as classical marker of inflammation was also ~10-fold increased in UC patients compared to the very low backgroundexpression levels found in non-UC controls (P<0.05).CONCLUSION: Expression of KV1.3 and, possibly, KCa3.1 channels are increased in a disease related fashion in UC patients and may point to an increased infiltration and activity of effector T-cells and effector memory T-cells in UC.These markers could be predictive of progression and for adjusting individual immune suppressive treatments.

KW - colitis ulcerosa

KW - translationel forskning

M3 - Poster

ER -

Hansen LK, Larsen D, Sadda V, Nielsen G, Klinge L, Schaffalitzky de Muckadell OB et al. Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis. 2011. Poster session presented at United European Gastroenterology Week (UEGW) 2011, Stockholm, Sweden.