Increased expression of T-cell KV1.3 and KCa3.1 channels in the inflamed intestinal wall from patients with active ulcerative colitis

Lars Koch Hansen, Dorte Larsen, Veeranjaneyulu Sadda, Gorm Nielsen, Lone Klinge, Ove B. Schaffalitzky de Muckadell, Torben Knudsen, Jens Kjeldsen, Ralf Köhler

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

INTRODUCTION: T-cell KV1.3 and KCa3.1 channels have been proposed to be important effector proteins during T-cell activation and also in autoimmune disease by controlling T-cell motility, cytokine production, and proliferation. The role of KV1.3 channels in ulcerative colitis (UC) has not been defined yet. In the present study we hypothesized that T-cell KV1.3 and KCa3.1 expression may serve as novel molecular marker for T-cell infiltration, inflammatory activity, and
possibly disease progression.
AIMS & METHODS: Biopsies were obtained from the rectal mucosa in seven UC patients with clinical and endoscopic active disease and seven non-UC patients. Active inflammation in the rectal mucosa in patients with UC was verified histologically. Expression of KV1.3 and KCa3.1 genes and of INFγ as a classical marker of inflammation was investigated in mRNA-extracts from biopsies by quantitative RT-PCR (qPCR).
RESULTS: Expression of KV1.3 was significantly ~7-foldly increased in UC patients compared to the very low background expression in controls (P<0.05). KCa3.1 gene expression tended also to be 2-foldly increased in UC patients
compared to the basal physiological expression in intestinal epithelium of controls (P=0.1). Expression of INFγ as classical marker of inflammation was also ~10-fold increased in UC patients compared to the very low background
expression levels found in non-UC controls (P<0.05).
CONCLUSION: Expression of KV1.3 and, possibly, KCa3.1 channels are increased in a disease related fashion in UC patients and may point to an increased infiltration and activity of effector T-cells and effector memory T-cells in UC.
These markers could be predictive of progression and for adjusting individual immune suppressive treatments.
Translated title of the contributionØget ekspression af T-celle KV1.3 og KCa3.1 kanaler i den inflammerede tarmslinhinde hos patienter med aktiv colitis ulcerosa
Original languageEnglish
Publication date22. Oct 2011
Number of pages1
Publication statusPublished - 22. Oct 2011
EventUnited European Gastroenterology Week (UEGW) 2011 - Stockholm, Sweden
Duration: 22. Oct 201126. Oct 2011

Conference

ConferenceUnited European Gastroenterology Week (UEGW) 2011
Country/TerritorySweden
CityStockholm
Period22/10/201126/10/2011

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