Peter Højrup

2024

Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls

Larsen, D. N., Kaczmarek, J. Z., Palarasah, Y., Graversen, J. H. & Højrup, P., 1. Jul 2024, In: BBA Proteins and Proteomics. 1872, 4, 141011.

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Increased Protein Kinase A Activity Induces Fibrolamellar Hepatocellular Carcinoma Features Independent of DNAJB1

Shirani, M., Levin, S., Shebl, B., Requena, D., Finkelstein, T. M., Johnson, D. S., Ng, D., Lalazar, G., Heissel, S., Hojrup, P., Molina, H., de Jong, Y. P., Rice, C. M., Singhi, A. D., Torbenson, M. S., Coffino, P., Lyons, B. & Simon, S. M., 15. Aug 2024, In: Cancer Research. 84, 16, p. 2626-2644

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2023

Antibodies to calnexin and mutated calreticulin are common in human sera

Kyllesbech, C., Trier, N., Mughal, F., Hansen, P., Holmström, M., el Fassi, D., Hasselbalch, H., Skov, V., Kjær, L., Andersen, M., Ciplys, E., Slibinskas, R., Frederiksen, J., Højrup, P. & Houen, G., 1. Apr 2023, In: Current Research in Translational Medicine. 71, 2, 103380.

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Citrullination of myelin basic protein induces a Th17-cell response in healthy individuals and enhances the presentation of MBP85-99 in patients with multiple sclerosis

Martín Monreal, M. T., Hansen, B. E., Iversen, P. F., Enevold, C., Ødum, N., Sellebjerg, F., Højrup, P., Rode von Essen, M. & Nielsen, C. H., Sept 2023, In: Journal of Autoimmunity. 139, 11 p., 103092.

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Defensin Interactions in Relation to Monoclonal and Disease-Related Proteinase 3 Antibodies Binding at the Catalytic Site

Zoega, M., Trier, N. H., Nejrup, R. G., Chailyan, A., Friis, T., Højrup, P. & Houen, G., 13. Mar 2023, In: Antibodies. 12, 1, 25 p., 23.

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Elevated Antibody Titers to Epstein–Barr Virus and Cytomegalovirus in Patients with Drug-Induced Lupus

Knudsen, J., Trier, N. H., Draborg, A. H., Nielsen, C. T., Jacobsen, S., Højrup, P. & Houen, G., 17. Apr 2023, In: Viruses. 15, 4, 10 p., 986.

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2022

Comparative Analysis of Type 1 and Type Z Protein Phosphatases Reveals D615 as a Key Residue for Ppz1 Regulation

Casamayor, A., Velázquez, D., Santolaria, C., Albacar, M., Rasmussen, M. I., Højrup, P. & Ariño, J., Feb 2022, In: International Journal of Molecular Sciences . 23, 3, 1327.

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Haptoglobin-related protein in human plasma correlates to haptoglobin concentrations and phenotypes

Skytthe, M. K., Sørensen, A. L., Hennig, D., Sandberg, M. B., Rasmussen, L. M., Højrup, P., Møller, H. J., Skjødt, K., Moestrup, S. K. & Graversen, J. H., Sept 2022, In: Scandinavian Journal of Clinical and Laboratory Investigation. 82, 6, p. 461-466

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Production and characterization of peptide antibodies to the C-terminal of frameshifted calreticulin associated with myeloproliferative diseases

Mughal, F. P., Bergmann, A. C., Huynh, H. U. B., Jørgensen, S. H., Mansha, I., Kesmez, M., Schürch, P. M., Theocharides, A. P. A., Hansen, P. R., Friis, T., Holmström, M. O., Ciplys, E., Slibinskas, R., Højrup, P., Houen, G. & Trier, N. H., Jun 2022, In: International Journal of Molecular Sciences . 23, 12, 6803.

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Tyrosine-sulfated dermatopontin shares multiple binding sites and recognition determinants on triple-helical collagens with proteins implicated in cell adhesion and collagen folding, fibrillogenesis, cross-linking, and degradation

Jensen, M. M., Bonna, A., Frederiksen, S. J., Hamaia, S. W., Højrup, P., Farndale, R. W. & Karring, H., 1. May 2022, In: BBA Proteins and Proteomics. 1870, 5, 140771.

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2021

Epitope mapping of monoclonal antibodies to calreticulin reveals that charged amino acids are essential for antibody binding

Bergmann, A. C., Kyllesbech, C., Slibinskas, R., Ciplys, E., Højrup, P., Trier, N. H. & Houen, G., 2021, In: Antibodies. 10, 3, 13 p., 31.

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Mapping human calreticulin regions important for structural stability

Čiplys, E., Paškevičius, T., Žitkus, E., Bielskis, J., Ražanskas, R., Šneideris, T., Smirnovas, V., Kaupinis, A., Tester, D. J., Ackerman, M. J., Højrup, P., Michalak, M., Houen, G. & Slibinskas, R., Nov 2021, In: BBA Proteins and Proteomics. 1869, 11, 18 p., 140710.

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2020

Cannabidiol is an effective helper compound in combination with bacitracin to kill Gram-positive bacteria

Wassmann, C. S., Højrup, P. & Klitgaard, J. K., 5. Mar 2020, In: Scientific Reports. 10, 12 p., 4112.

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Citrullinome of Porphyromonas gingivalis Outer Membrane Vesicles: Confident Identification of Citrullinated Peptides

Larsen, D. N., Mikkelsen, C. E., Kierkegaard, M., Bereta, G. P., Nowakowska, Z., Kaczmarek, J. Z., Potempa, J. & Højrup, P., Jan 2020, In: Molecular and Cellular Proteomics. 19, 1, p. 167-180 14 p.

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2019

An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

Skottrup, P. D., López, R., Ksiazek, M., Højrup, P., Baelum, V., Potempa, J. & Kaczmarek, J. Z., Jun 2019, In: Journal of Immunological Methods. 469, p. 26-32

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Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications

Heissel, S., Frederiksen, S. J., Bunkenborg, J. & Højrup, P., 27. Jun 2019, In: PLOS ONE. 14, 6, 16 p., e0218374.

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Immunoglobulin G structure and rheumatoid factor epitopes

Maibom-Thomsen, S. L., Trier, N. H., Holm, B. E., Hansen, K. B., Rasmussen, M. I., Chailyan, A., Marcatili, P., Højrup, P. & Houen, G., 14. Jun 2019, In: PLOS ONE. 14, 6, 28 p., e0217624.

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2018

Chaperone-client interactions between Hsp21 and client proteins monitored in solution by small angle X-ray scattering and captured by crosslinking mass spectrometry

Rutsdottir, G., I Rasmussen, M., Hojrup, P., Bernfur, K., Emanuelsson, C. & Söderberg, C. A. G., 2018, In: Proteins. 86, 1, p. 110-123

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Discrimination of Isoleucine and Leucine by Dimethylation-Assisted MS3

Maibom-Thomsen, S., Heissel, S., Mørtz, E., Højrup, P. & Bunkenborg, J., 2018, In: Analytical Chemistry. 90, 15, p. 9055–9059

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Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein

Heissel, S., Bunkenborg, J., Kristiansen, M. P., Holmbjerg, A. F., Grimstrup, M., Mørtz, E., Kofoed, T. & Højrup, P., 2018, In: Protein Expression and Purification. 147, p. 69-77

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Structural modelling of the DNAJB6 oligomeric chaperone shows a peptide-binding cleft lined with conserved S/T-residues at the dimer interface

Söderberg, C. A. G., Månsson, C., Bernfur, K., Rutsdottir, G., Härmark, J., Rajan, S., Al-Karadaghi, S., Rasmussen, M., Höjrup, P., Hebert, H. & Emanuelsson, C., 2018, In: Scientific Reports. 8, 15 p., 5199.

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XK-related protein 5 (XKR5) is a novel negative regulator of KIT/D816V-mediated transformation

Sun, J., Thingholm, T., Højrup, P. & Rönnstrand, L., 18. Jun 2018, In: Oncogenesis. 7, 6, 11 p., 48.

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2017

Characterization of equine vitamin D–binding protein, development of an assay, and assessment of plasma concentrations of the protein in healthy horses and horses with gastrointestinal disease

Pihl, T. H., Jacobsen, S., Olsen, D. T., Højrup, P., Grosche, A., Freeman, D. E., Andersen, P. H. & Houen, G., 2017, In: American Journal of Veterinary Research. 78, 6, p. 718-728

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Iron-induced oligomerization of human FXN^81-210 and bacterial CyaY frataxin and the effect of iron chelators

Ahlgren, E. C., Fekry, M., Wiemann, M., Söderberg, C. A., Bernfur, K., Gakh, O., Rasmussen, M., Højrup, P., Emanuelsson, C., Isaya, G. & Al-Karadaghi, S., 2017, In: PLOS ONE. 12, 12, 25 p., e0188937.

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Structural model of dodecameric heat-shock protein Hsp21: Flexible N-terminal arms interact with client proteins while C-terminal tails maintain the dodecamer and chaperone activity

Rutsdottir, G., Härmark, J., Weide, Y., Hebert, H., Rasmussen, M. I., Wernersson, S., Respondek, M., Akke, M., Højrup, P., Koeck, P. J. B., Söderberg, C. A. G. & Emanuelsson, C., 2017, In: Journal of Biological Chemistry. 292, 19, p. 8103-8121

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2016

Exploring structure and interactions of the bacterial adaptor protein YjbH by crosslinking mass spectrometry

Al-Eryani, Y., Ib Rasmussen, M., Kjellström, S., Højrup, P., Emanuelsson, C. & von Wachenfeldt, C., Sept 2016, In: Proteins. 84, 9, p. 1234-1245

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Mapping the Ca²⁺ induced structural change in calreticulin

Boelt, S. G., Norn, C., Rasmussen, M. I., André, I., Čiplys, E., Slibinskas, R., Houen, G. & Højrup, P., 16. Jun 2016, In: Journal of Proteomics. 142, p. 138-148

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2015

[(64) Cu]-labelled trastuzumab: optimisation of labelling by DOTA and NODAGA conjugation and initial evaluation in mice

Schjoeth-Eskesen, C., Nielsen, C. H., Heissel, S., Højrup, P., Hansen, P. R., Gillings, N. & Kjaer, A., 2015, In: Journal of Labelled Compounds and Radiopharmaceuticals. 58, 6, p. 227-233

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Agarose gel shift assay reveals that calreticulin favors substrates with a quaternary structure in solution

Boelt, S. G., Houen, G. & Højrup, P., 2015, In: Analytical Biochemistry. 481, p. 33-42

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High-level secretion of native recombinant human calreticulin in yeast

Čiplys, E., Žitkus, E., Gold, L. I., Daubriac, J., Pavlides, S. C., Højrup, P., Houen, G., Wang, W.-A., Michalak, M. & Slibinskas, R., 2015, In: Microbial Cell Factories. 14, October, 14 p., 165.

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2014

Characterization of an extensin-modifying metalloprotease: N-terminal processing and substrate cleavage pattern of Pectobacterium carotovorum Prt1

Feng, T., Nyffenegger, C., Højrup, P., Vidal-Melgosa, S., Yan, K.-P., Fangel, J. U., Meyer, A. S., Kirpekar, F., Willats, W. G. & Mikkelsen, J. D., 20. Nov 2014, In: Applied Microbiology and Biotechnology. 98, 24, p. 10077-10089

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Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy

Nalla, A., Ringholm, L., Søstrup, B., Højrup, P., Thim, L., Levery, S. B., Vakhrushev, S. Y., Billestrup, N., Mathiesen, E. R., Damm, P. & Nielsen, J. H., Nov 2014, In: Acta Obstetricia et Gynecologica Scandinavica. 93, 11, p. 1181-1189 9 p.

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Probing the structure of human protein disulfide isomerase by chemical cross-linking combined with mass spectrometry

Peng, L., Rasmussen, M. I., Chailyan, A., Houen, G. & Højrup, P., 28. Aug 2014, In: Journal of Proteomics. 108, August, p. 1-16 16 p.

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Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered

Poulsen, E. T., Dyrlund, T. F., Runager, K., Scavenius, C., Krogager, T. P., Højrup, P., Thøgersen, I. B., Sanggaard, K. W., Vorum, H., Hjortdal, J. & Enghild, J. J., 7. Nov 2014, In: Journal of Proteome Research. 13, 11, p. 4659–4667

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Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

Vinther, L., Lademann, U., Andersen, E. V., Højrup, P., Thaysen-Andersen, M., Krogh, B. O., Viuff, B., Brünner, N., Stenvang, J. & Moreira, J. M. A., Sept 2014, In: Protein Expression and Purification. 101, p. 157-164 8 p.

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2013

Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway

Güven, E., Duus, K., Laursen, I., Højrup, P. & Houen, G., 2013, In: PLOS ONE. 8, 9, p. e74445

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A Selected Reaction Monitoring (SRM)-Based Method for Absolute Quantification of Aβ38, Aβ40, and Aβ42 in Cerebrospinal Fluid of Alzheimer's Disease Patients and Healthy Controls

Pannee, J., Portelius, E., Oppermann, M., Atkins, A., Hornshaw, M., Zegers, I., Højrup, P., Minthon, L., Hansson, O., Zetterberg, H., Blennow, K. & Gobom, J., 2013, In: Journal of Alzheimer's Disease. 33, 4, p. 1021-1032

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Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)

Jensen, P. H., Mysling, S., Højrup, P. & Jensen, O. N., 2013, In: Methods in Molecular Biology. 951, p. 131-144

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Identification of a laccase from Ganoderma lucidum CBS 229.93 having potential for enhancing cellulase catalyzed lignocellulose degradation

Sitarz, A. K., Mikkelsen, J. D., Højrup, P. & Meyer, A. S., 10. Dec 2013, In: Enzyme and Microbial Technology. 53, 6-7, p. 378-385

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Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems

Albrethsen, J., Agner, J., Piersma, S. R., Højrup, P., Pham, T. V., Weldingh, K., Jimenez, C. R., Andersen, P. & Rosenkrands, I., 2013, In: Molecular and Cellular Proteomics. 12, 5, p. 1180-1191

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Purification and characterization of a soluble calnexin from human placenta

Olsen, D. T., Peng, L., Træholt, S. D., Duus, K., Højrup, P. & Houen, G., 2013, In: Protein Expression and Purification. 92, 1, p. 105-111

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Serine protease HtrA1 accumulates in corneal transforming growth factor beta induced protein (TGFBIp) amyloid deposits

Karring, H., Poulsen, E. T., Runager, K., Thøgersen, I. B., Klintworth, G. K., Højrup, P. & Enghild, J. J., 2013, In: Molecular Vision. 19, p. 861-876

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Towards the N-terminal acetylome: an N-terminal acetylated peptide enrichment method using CNBr-activated sepharose resin

Zhang, X. & Højrup, P., 2013, In: Methods in Molecular Biology. 981, p. 47-56

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2012

A Single Rainbow Trout Cobalamin-binding Protein Stands in for Three Human Binders

Greibe, E. H., Fedosov, S., Sorensen, B. S., Højrup, P., Poulsen, S. S. & Nexo, E., 2012, In: Journal of Biological Chemistry. 287, 40, p. 33917-25 9 p.

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Composition and proteolytic processing of corneal deposits associated with mutations in the TGFBI gene

Karring, H., Runager, K., B. Thøgersen, I., K. Klintworth, G., Højrup, P. & Enghild, J. J., 2012, In: Experimental Eye Research. 96, 1, p. 163-170

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Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Krogager, T. P., Nielsen, L. V., Bak, S., Young, C., Ferreri, C., Jensen, O. N., Højrup, P., Thoma, V., Thøgersen, I. & Enghild, J. J., 2012, In: Journal of Proteomics. 75, 9, p. 2685-96 12 p.

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Proteinase 3 carries small unusual carbohydrates and associates with αlpha-defensins

Zoega, M., Ravnsborg, T., Højrup, P., Houen, G. & Schou, C., 2012, In: Journal of Proteomics. 75, 5, p. 1472-1485

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The protein composition of the digestive fluid from the Venus flytrap sheds light on prey digestion mechanisms.

Schulze, W. X., Sanggaard, K. W., Kreuzer, I., Knudsen, A. D., Bemm, F., Thøgersen, I. B., Bräutigam, A., Thomsen, L. R., Schliesky, S., Dyrlund, T. F., Escalante-Perez, M., Becker, D., Schultz, J., Karring, H., Weber, A., Højrup, P., Hedrich, R. & Enghild, J. J., 2012, In: Molecular and Cellular Proteomics. 11, 11, p. 1306-1319 14 p.

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Time-dependent changes in protein expression in rainbow trout muscle following hypoxia

Wulff, T., Jokumsen, A., Højrup, P. & Jessen, F., 2012, In: Journal of Proteomics. 75, 8, p. 2342-51 10 p.

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2011

A proteome-scale study on in vivo protein N(α)-acetylation using an optimized method.

Zhang, X., Engholm-Keller, K. & Højrup, P., 2011, In: Proteomics. 11, p. 81-93 14 p.

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Peter Højrup

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