Peter Højrup

ph.d.

  • Campusvej 55

    5230 Odense M

    Denmark

1982 …2020
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Research Output 1982 2020

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Journal article
2020

Cannabidiol is an effective helper compound in combination with bacitracin to kill Gram-positive bacteria

Wassmann, C. S., Højrup, P. & Klitgaard, J. K., 19. Feb 2020, (Accepted/In press) In : Scientific Reports.

Research output: Contribution to journalJournal articleResearchpeer-review

Citrullinome of Porphyromonas gingivalis Outer Membrane Vesicles: Confident Identification of Citrullinated Peptides

Larsen, D. N., Mikkelsen, C. E., Kierkegaard, M., Bereta, G. P., Nowakowska, Z., Kaczmarek, J. Z., Potempa, J. & Højrup, P., Jan 2020, In : Molecular & cellular proteomics : MCP. 19, 1, p. 167-180 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Porphyromonas gingivalis
Membranes
Peptides
Chronic Periodontitis
Periplasm
2019

An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

Skottrup, P. D., López, R., Ksiazek, M., Højrup, P., Baelum, V., Potempa, J. & Kaczmarek, J. Z., Jun 2019, In : Journal of Immunological Methods. 469, p. 26-32

Research output: Contribution to journalJournal articleResearchpeer-review

Saliva
Immunoassay
Periodontitis
Point-of-Care Systems
Surface Plasmon Resonance
91 Downloads (Pure)

Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications

Heissel, S., Frederiksen, S. J., Bunkenborg, J. & Højrup, P., 27. Jun 2019, In : PLOS ONE. 14, 6, 16 p., e0218374.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
High temperature applications
proteomics
Trypsin
trypsin
Purification
107 Downloads (Pure)

Immunoglobulin G structure and rheumatoid factor epitopes

Maibom-Thomsen, S. L., Trier, N. H., Holm, B. E., Hansen, K. B., Rasmussen, M. I., Chailyan, A., Marcatili, P., Højrup, P. & Houen, G., 14. Jun 2019, In : PLOS ONE. 14, 6, 28 p., e0217624.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Rheumatoid Factor
immunoglobulin G
epitopes
Epitopes
Immunoglobulin G
2018

Chaperone-client interactions between Hsp21 and client proteins monitored in solution by small angle X-ray scattering and captured by crosslinking mass spectrometry

Rutsdottir, G., I Rasmussen, M., Hojrup, P., Bernfur, K., Emanuelsson, C. & Söderberg, C. A. G., 2018, In : Proteins. 86, 1, p. 110-123

Research output: Contribution to journalJournal articleResearchpeer-review

X ray scattering
Crosslinking
Mass spectrometry
X-Rays
Small Heat-Shock Proteins
64 Downloads (Pure)

Discrimination of Isoleucine and Leucine by Dimethylation-Assisted MS3

Maibom-Thomsen, S., Heissel, S., Mørtz, E., Højrup, P. & Bunkenborg, J., 2018, In : Analytical Chemistry. 90, 15, p. 9055–9059

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Isoleucine
Leucine
Peptides
Ions
Thermolysin
74 Downloads (Pure)

Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein

Heissel, S., Bunkenborg, J., Kristiansen, M. P., Holmbjerg, A. F., Grimstrup, M., Mørtz, E., Kofoed, T. & Højrup, P., 2018, In : Protein Expression and Purification. 147, p. 69-77

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Recombinant Proteins
Libraries
Proteins
Workflow
Isotopes
180 Downloads (Pure)

Structural modelling of the DNAJB6 oligomeric chaperone shows a peptide-binding cleft lined with conserved S/T-residues at the dimer interface

Söderberg, C. A. G., Månsson, C., Bernfur, K., Rutsdottir, G., Härmark, J., Rajan, S., Al-Karadaghi, S., Rasmussen, M., Höjrup, P., Hebert, H. & Emanuelsson, C., 2018, In : Scientific Reports. 8, 15 p., 5199.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Peptides
Isotopes
Lysine
Electron Microscopy
X-Rays
105 Downloads (Pure)

XK-related protein 5 (XKR5) is a novel negative regulator of KIT/D816V-mediated transformation

Sun, J., Thingholm, T., Højrup, P. & Rönnstrand, L., 18. Jun 2018, In : Oncogenesis. 7, 6, 11 p., 48.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Proteins
Tyrosine
Down-Regulation
2017

Characterization of equine vitamin D–binding protein, development of an assay, and assessment of plasma concentrations of the protein in healthy horses and horses with gastrointestinal disease

Pihl, T. H., Jacobsen, S., Olsen, D. T., Højrup, P., Grosche, A., Freeman, D. E., Andersen, P. H. & Houen, G., 2017, In : American Journal of Veterinary Research. 78, 6, p. 718-728

Research output: Contribution to journalJournal articleResearchpeer-review

Gastrointestinal Diseases
digestive system diseases
Vitamins
Horses
vitamins
124 Downloads (Pure)

Iron-induced oligomerization of human FXN81-210 and bacterial CyaY frataxin and the effect of iron chelators

Ahlgren, E. C., Fekry, M., Wiemann, M., Söderberg, C. A., Bernfur, K., Gakh, O., Rasmussen, M., Højrup, P., Emanuelsson, C., Isaya, G. & Al-Karadaghi, S., 2017, In : PLOS ONE. 12, 12, 25 p., e0188937.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Oligomerization
Chelating Agents
chelating agents
Oligomers
Iron
112 Downloads (Pure)

Structural model of dodecameric heat-shock protein Hsp21: Flexible N-terminal arms interact with client proteins while C-terminal tails maintain the dodecamer and chaperone activity

Rutsdottir, G., Härmark, J., Weide, Y., Hebert, H., Rasmussen, M. I., Wernersson, S., Respondek, M., Akke, M., Højrup, P., Koeck, P. J. B., Söderberg, C. A. G. & Emanuelsson, C., 2017, In : Journal of Biological Chemistry. 292, 19, p. 8103-8121

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Small Heat-Shock Proteins
Heat-Shock Proteins
Protein C
Plant Structures
Oligomerization
2016

Exploring structure and interactions of the bacterial adaptor protein YjbH by crosslinking mass spectrometry

Al-Eryani, Y., Ib Rasmussen, M., Kjellström, S., Højrup, P., Emanuelsson, C. & von Wachenfeldt, C., Sep 2016, In : Proteins. 84, 9, p. 1234-1245

Research output: Contribution to journalJournal articleResearchpeer-review

Crosslinking
Mass spectrometry
Proteins
Peptide Hydrolases
Lysine

Mapping the Ca2+ induced structural change in calreticulin

Boelt, S. G., Norn, C., Rasmussen, M. I., André, I., Čiplys, E., Slibinskas, R., Houen, G. & Højrup, P., 16. Jun 2016, In : Journal of Proteomics. 142, p. 138-148

Research output: Contribution to journalJournal articleResearchpeer-review

Calreticulin
Reticulin
Reticulum
Peptides
Biological systems
2015

[(64) Cu]-labelled trastuzumab: optimisation of labelling by DOTA and NODAGA conjugation and initial evaluation in mice

Schjoeth-Eskesen, C., Nielsen, C. H., Heissel, S., Højrup, P., Hansen, P. R., Gillings, N. & Kjaer, A., 2015, In : Journal of Labelled Compounds and Radiopharmaceuticals. 58, 6, p. 227-33

Research output: Contribution to journalJournal articleResearchpeer-review

Labeling
Tumors
Chelating Agents
Trastuzumab
1-(1,3-carboxypropyl)-4,7-carboxymethyl-1,4,7-triazacyclononane

Agarose gel shift assay reveals that calreticulin favors substrates with a quaternary structure in solution

Boelt, S. G., Houen, G. & Højrup, P., 2015, In : Analytical Biochemistry. 481, p. 33-42

Research output: Contribution to journalJournal articleResearchpeer-review

Calreticulin
Sepharose
Assays
Gels
Substrates
131 Downloads (Pure)

High-level secretion of native recombinant human calreticulin in yeast

Čiplys, E., Žitkus, E., Gold, L. I., Daubriac, J., Pavlides, S. C., Højrup, P., Houen, G., Wang, W-A., Michalak, M. & Slibinskas, R., 2015, In : Microbial Cell Factories. 14, October, 14 p., 165.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Calreticulin
Yeast
Proteins
Recombinant proteins
Saccharomyces cerevisiae
2014

Characterization of an extensin-modifying metalloprotease: N-terminal processing and substrate cleavage pattern of Pectobacterium carotovorum Prt1

Feng, T., Nyffenegger, C., Højrup, P., Vidal-Melgosa, S., Yan, K-P., Fangel, J. U., Meyer, A. S., Kirpekar, F., Willats, W. G. & Mikkelsen, J. D., 2014, In : Applied Microbiology and Biotechnology. 98, p. 10077-10089

Research output: Contribution to journalJournal articleResearchpeer-review

Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy

Nalla, A., Ringholm, L., Søstrup, B., Højrup, P., Thim, L., Levery, S. B., Vakhrushev, S. Y., Billestrup, N., Mathiesen, E. R., Damm, P. & Nielsen, J. H., Nov 2014, In : Acta Obstetricia et Gynecologica Scandinavica. 93, 11, p. 1181-9 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Serum
Pregnant Women
Hormones
Placental Lactogen
Peptides

Probing the structure of human protein disulfide isomerase by chemical cross-linking combined with mass spectrometry

Peng, L., Rasmussen, M. I., Chailyan, A., Houen, G. & Højrup, P., 2. May 2014, In : Journal of Proteomics. 108, August, p. 1-16 16 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Protein Disulfide-Isomerases
Mass spectrometry
Calnexin
Proteins
Molecules

Proteomics of Fuchs' Endothelial Corneal Dystrophy support that the extracellular matrix of Descemet's membrane is disordered

Poulsen, E. T., Dyrlund, T. F., Runager, K., Scavenius, C., Krogager, T. P., Højrup, P., Thøgersen, I. B., Sanggaard, K. W., Vorum, H., Hjortdal, J. & Enghild, J. J., 2014, In : Journal of Proteome Research. 13, 11, p. 4659–4667

Research output: Contribution to journalJournal articleResearchpeer-review

Fuchs' Endothelial Dystrophy
Descemet Membrane
Membranes
Proteins
Tissue

Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells

Vinther, L., Lademann, U., Andersen, E. V., Højrup, P., Thaysen-Andersen, M., Krogh, B. O., Viuff, B., Brünner, N., Stenvang, J. & Moreira, J. M. A., Sep 2014, In : Protein Expression and Purification. 101, p. 157-64 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Tissue Inhibitor of Metalloproteinase-1
Proteins
Glycosylation
Matrix Metalloproteinase Inhibitors
HEK293 Cells
2013

Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway

Güven, E., Duus, K., Laursen, I., Højrup, P. & Houen, G., 2013, In : PLOS ONE. 8, 9, p. e74445

Research output: Contribution to journalJournal articleResearchpeer-review

Aluminum Hydroxide
aluminum hydroxide
adjuvants
complement
Chemical activation

A Selected Reaction Monitoring (SRM)-Based Method for Absolute Quantification of Aβ38, Aβ40, and Aβ42 in Cerebrospinal Fluid of Alzheimer's Disease Patients and Healthy Controls

Pannee, J., Portelius, E., Oppermann, M., Atkins, A., Hornshaw, M., Zegers, I., Højrup, P., Minthon, L., Hansson, O., Zetterberg, H., Blennow, K. & Gobom, J., 2013, In : Journal of Alzheimer's Disease. 33, 4, p. 1021-1032

Research output: Contribution to journalJournal articleResearchpeer-review

Cerebrospinal Fluid
Alzheimer Disease
Research
Clinical Trials
Peptides

Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)

Jensen, P. H., Mysling, S., Højrup, P. & Jensen, O. N., 2013, In : Methods in Molecular Biology. 951, p. 131-144

Research output: Contribution to journalJournal articleResearchpeer-review

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Solid Phase Extraction
Liquid Chromatography
Ions
Peptides

Identification of a laccase from Ganoderma lucidum CBS 229.93 having potential for enhancing cellulase catalyzed lignocellulose degradation

Sitarz, A. K., Mikkelsen, J. D., Højrup, P. & Meyer, A. S., 10. Dec 2013, In : Enzyme and Microbial Technology. 53, 6-7, p. 378-85

Research output: Contribution to journalJournal articleResearchpeer-review

Reishi
Sugar cane
Laccase
Bagasse
Cellulase

Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems

Albrethsen, J., Agner, J., Piersma, S. R., Højrup, P., Pham, T. V., Weldingh, K., Jimenez, C. R., Andersen, P. & Rosenkrands, I., 2013, In : Molecular and Cellular Proteomics. 12, 5, p. 1180-1191

Research output: Contribution to journalJournal articleResearchpeer-review

Antitoxins
Mycobacterium tuberculosis
Nutrients
Food
Proteins

Purification and characterization of a soluble calnexin from human placenta

Olsen, D. T., Peng, L., Træholt, S. D., Duus, K., Højrup, P. & Houen, G., 2013, In : Protein Expression and Purification. 92, 1, p. 105-11

Research output: Contribution to journalJournal articleResearchpeer-review

Calnexin
Calreticulin
Placenta
Ion Exchange Chromatography
Protease Inhibitors

Serine protease HtrA1 accumulates in corneal transforming growth factor beta induced protein (TGFBIp) amyloid deposits

Karring, H., Poulsen, E. T., Runager, K., Thøgersen, I. B., Klintworth, G. K., Højrup, P. & Enghild, J. J., 2013, In : Molecular Vision. 19, p. 861-76

Research output: Contribution to journalJournal articleResearchpeer-review

Towards the N-terminal acetylome: an N-terminal acetylated peptide enrichment method using CNBr-activated sepharose resin

Zhang, X. & Højrup, P., 2013, In : Methods in Molecular Biology. 981, p. 47-56

Research output: Contribution to journalJournal articleResearchpeer-review

Sepharose
Peptides
Proteome
Acetylation
Proteins
2012

A Single Rainbow Trout Cobalamin-binding Protein Stands in for Three Human Binders

Greibe, E. H., Fedosov, S., Sorensen, B. S., Højrup, P., Poulsen, S. S. & Nexo, E., 2012, In : Journal of Biological Chemistry. 287, 40, p. 33917-25 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Transcobalamins
Oncorhynchus mykiss
Binders
Trout
Intrinsic Factor

Composition and proteolytic processing of corneal deposits associated with mutations in the TGFBI gene

Karring, H., Runager, K., B. Thøgersen, I., K. Klintworth, G., Højrup, P. & Enghild, J. J., 2012, In : Experimental Eye Research. 96, 1, p. 163-170

Research output: Contribution to journalJournal articleResearchpeer-review

Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Krogager, T. P., Nielsen, L. V., Bak, S., Young, C., Ferreri, C., Jensen, O. N., Højrup, P., Thoma, V., Thøgersen, I. & Enghild, J. J., 2012, In : Journal of Proteomics. 75, 9, p. 2685-96 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Isotope Labeling
Dietary Fats
Biomarkers
Cell culture
Isotopes

Proteinase 3 carries small unusual carbohydrates and associates with αlpha-defensins

Zoega, M., Ravnsborg, T., Højrup, P., Houen, G. & Schou, C., 2012, In : Journal of Proteomics. 75, 5, p. 1472-1485

Research output: Contribution to journalJournal articleResearchpeer-review

Myeloblastin
Defensins
Carbohydrates
Neutrophils
Glycosylation

The protein composition of the digestive fluid from the Venus flytrap sheds light on prey digestion mechanisms.

Schulze, W. X., Sanggaard, K. W., Kreuzer, I., Knudsen, A. D., Bemm, F., Thøgersen, I. B., Bräutigam, A., Thomsen, L. R., Schliesky, S., Dyrlund, T. F., Escalante-Perez, M., Becker, D., Schultz, J., Karring, H., Weber, A., Højrup, P., Hedrich, R. & Enghild, J. J., 2012, In : Molecular and Cellular Proteomics. 11, 11, p. 1306-1319 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Time-dependent changes in protein expression in rainbow trout muscle following hypoxia

Wulff, T., Jokumsen, A., Højrup, P. & Jessen, F., 2012, In : Journal of Proteomics. 75, 8, p. 2342-51 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Oncorhynchus mykiss
Muscle
Muscles
Proteins
Proteome
2011

A proteome-scale study on in vivo protein N(α)-acetylation using an optimized method.

Zhang, X., Engholm-Keller, K. & Højrup, P., 2011, In : Proteomics. 11, p. 81-93 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A proteome-scale study on in vivo protein Nα-acetylation using an optimized method

Zhang, X., Ye, J., Engholm-Keller, K. & Højrup, P., 1. Jan 2011, In : Proteomics. 11, 1, p. 81-93 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Acetylation
Proteome
Peptides
Resins
Proteins

Assessing high affinity binding to HLA-DQ2.5 by a novel peptide library based approach

Jüse, U., Arntzen, M., Højrup, P., Fleckenstein, B. & Sollid, L. M., 1. Apr 2011, In : Bioorganic & Medicinal Chemistry. 19, 7, p. 2470-7 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Peptide Library
Libraries
Mass spectrometry
Search Engine
Peptides

CrossWork: Software-assisted identification of cross-linked peptides

Rasmussen, M., Refsgaard, J., Peng, L., Houen, G. & Højrup, P., 2011, In : Journal of Proteomics. 74, 10, p. 1871-1883 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Determination of the binding sites for oxaliplatin on insulin using mass spectrometry-based approaches

Møller, C., Sprenger, R. R., Stürup, S. & Højrup, P., 19. Jul 2011, In : Analytical and Bioanalytical Chemistry. 401, 5, p. 1623-1633 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

oxaliplatin
Insulin
Binding sites
Mass spectrometry
Binding Sites

Human inter-α-inhibitor is a substrate for factor XIIIa and tissue transglutaminase

Sonne-Schmidt, C. S., Sanggaard, K. W., Nikolajsen, C. L., Bak, S., Valnickova, Z., Thøgersen, I., Jensen, O. N., Højrup, P. & Enghild, J. J., 2011, In : BBA General Subjects. 1814, 12, p. 1624-1630

Research output: Contribution to journalJournal articleResearchpeer-review

Factor XIIIa
Fibrin
Substrates
Proteolysis
Antifibrinolytic Agents

Quantitative proteomics by amino acid labeling in C. elegans

Fredens, J., Engholm-Keller, K., Giessing, A., Pultz, D., Larsen, M. R., Højrup, P., Møller-Jensen, J. & Færgeman, N. J., 2011, In : Nature Methods. 8, 10, p. 845-847

Research output: Contribution to journalJournal articleResearchpeer-review

Caenorhabditis elegans
Isotopes
Labeling
Amino Acids
Gene Knockdown Techniques

Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis

Holten-Andersen, L., Thaysen-Andersen, M., Jensen, S. B., Buchwald, C., Højrup, P., Offenberg, H., Nielsen, H. J., Brünner, N. A., Nauntofte, B. & Reibel, J., 1. Nov 2011, In : APMIS Supplementum. 119, 11, p. 741-749 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Tissue Inhibitor of Metalloproteinase-1
Glycosylation
Saliva
Parotid Gland
Messenger RNA

Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, HCD and ETD-MS applied to the N-linked glycoproteome of Campylobacter jejuni

Scott, N. E., Parker, B. L., Connolly, A. M., Paulech, J., Edwards, A. V. G., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S. P., Højrup, P., Packer, N. H., Larsen, M. R. & Cordwell, S. J., 2011, In : Molecular and Cellular Proteomics. 10, 2, p. M000031-MCP201

Research output: Contribution to journalJournal articleResearchpeer-review

Campylobacter jejuni
Chromatography
Glycosylation
Polysaccharides
Electrons

Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor

Kristiansen, E., Ramløv, H., Højrup, P., Pedersen, S. A., Hagen, L. & Zachariassen, K. E., 12. Nov 2011, In : Insect Biochemistry and Molecular Biology. 41, 2, p. 109-117

Research output: Contribution to journalJournal articleResearchpeer-review

Rhagium
Antifreeze Proteins
Coleoptera
Insect Proteins
insect proteins

The interactions of calreticulin with immunoglobulin G and immunoglobulin Y

Møllegaard, K. M., Duus, K., Træholt, S. D., Thaysen-Andersen, M., Liu, Y., Palma, A. S., Feizi, T., Hansen, P. R., Højrup, P. & Houen, G., 2011, In : BBA General Subjects. 1814, 7, p. 889-99 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Calreticulin
Immunoglobulin G
Peptides
Mannose
Oligosaccharides
2010

Cyclization of the N-Terminal X-Asn-Gly Motif during Sample Preparation for Bottom-Up Proteomics

Zhang, X. & Højrup, P., 2010, In : Analytical Chemistry. 82, 20, p. 8680-8685

Research output: Contribution to journalJournal articleResearchpeer-review

Cyclization
Peptides
Proteomics
Glutamine
222 Downloads (Pure)

Distinct patterns of blood-stage parasite antigens detected by plasma IgG subclasses from individuals with different level of exposure to Plasmodium falciparum infections

Olesen, C. H., Brahimi, K., Vandahl, B., Lousada-Dietrich, S., Jogdand, P. S., Vestergaard, L. S., Dodoo, D., Højrup, P., Christiansen, M., Larsen, S. O., Singh, S. & Theisen, M., 1. Jan 2010, In : Malaria Journal. 9, p. 296

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Plasmodium falciparum
Malaria
Parasites
Molecular Weight
Nonparametric Statistics