Peter Højrup

ph.d.

  • Campusvej 55

    5230 Odense M

    Denmark

1982 …2020
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Research Output 1982 2020

Book chapter

A General Strategy for the use of Mass Spectrometric Molecular Weight Information in Protein Purification and Sequence Determination

Roepstorff, P. & Højrup, P., 1993, Methods in Protein Sequence Analysis. Imahori, K. & Sakiyama, F. (eds.). Springer, p. 149-156

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Analysis of Peptides and Conjugates by Amino Acid Analysis

Højrup, P., 2015, Peptide Antibodies: Methods and Protocols. Houen, G. (ed.). Humana Press, p. 65-76 (Methods in Molecular Biology, Vol. 1348).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Amino Acids
Peptides
Ninhydrin
Ion Exchange Chromatography
Glutamine

Calibration of matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting spectra

Hjernø, K. & Højrup, P., 2007, Methods in Molecular Biology: Mass Spectrometry Data Analysis in Proteomics. Matthiesen, R. (ed.). 1 ed. AAAI Press, Vol. 367. p. 49-60 11 p.

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Peptides

Characterization of Synthetic Peptides by Mass Spectrometry

Prabhala, B. K., Mirza, O., Højrup, P. & Hansen, P. R., 2015, Peptide Antibodies: Methods and Protocols. Houen, G. (ed.). Springer, Vol. 1348. p. 77-82 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Peptides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tandem Mass Spectrometry

Essentiel bioinformatik: biologiske databaser

Højrup, P. & Hjernø, K., 2005, Proteiner: oprensning og karakterisering. Kielberg, V. & Rasmussen, L. (eds.). Copenhagen: Nordisk Forlag, p. 215-32

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis

Hjernø, K. & Højrup, P., 2015, Peptide antibodies: methods and protocols. Humana Press, Vol. 1348. p. 83-102 (Methods in Molecular Biology, Vol. 1348).

Research output: Chapter in Book/Report/Conference proceedingBook chapterCommunication

Tandem Mass Spectrometry
Peptides
Guidelines

Massespektrometri

Hjernø, K. & Højrup, P., 2005, Proteiner: oprensning og karakterisering. Kielberg, V. & Rasmussen, L. (eds.). Copenhagen: Nordisk Forlag, p. 195-214

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Molekylærbiologiske databaser

Højrup, P., 2008, Proteiner: Oprensning og karakterisering. Kielberg, V. & Rasmussen, L. (eds.). København: Gad, p. 171-184

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Molekylærbiologiske databaser

Højrup, P., 2003, DNA og RNA - en håndbog. Kielberg, V., Nørby, S. & Rasmussen, L. (eds.). København: Gad, p. 339-356

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

PeakErazor: A Windows-based programme for improving peptide mass searches.

Hjernø, K. & Højrup, P., 2004, Methods in Proteome and Protein Analysis.. Kamp (ed.). Berlin: Springer, p. 359-370

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Peptide Mapping for Protein Characterization

Højrup, P., 2009, The Protein Protocols Handbook. Totowa: Humana Press

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Proteolytic peptide mapping. HPLC of Peptides and Proteins.

Højrup, P., 2004, Methods in Molecular Biology. Aguilar, M-I. (ed.). Totowa, New Jersey: Humana Press, Vol. 251. p. 227-244

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Conference abstract for conference

COMPOSITION AND PROTEOLYTIC PROCESSING OF CORNEAL DEPOSITS ASSOCIATED WITH MUTATIONS IN THE TGFBI GENE

Karring, H., Poulsen, E. T., Runager, K., Klintworth, G. K., Højrup, P. & Enghild, J. J., 21. Jul 2012.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

PROTEOMICS OF CORNEAL DYSTROPHIES

Poulsen, E. T., Karring, H., Runager, K., Dyrlund, T. F., Klintworth, G. K., Højrup, P., Otzen, D. & Enghild, J. J., 2012.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

Serine Protease HtrA1 Accumulates in Corneal Transforming Growth Factor Beta Induced Protein (TGFBIp) Amyloid Deposits

Runager, K., Karring, H., Poulsen, E. T., Thøgersen, I. B., Klintworth, G. K., Højrup, P. & Enghild, J. J., 2013. 16 p.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

Structural characterization of heavily modified hydrophilic peptides from fish mucosa

Andersen, M. T., Caballol, M. A. O., Pedersen, K. L. & Højrup, P., 2005. 1 p.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearch

Journal article

[(64) Cu]-labelled trastuzumab: optimisation of labelling by DOTA and NODAGA conjugation and initial evaluation in mice

Schjoeth-Eskesen, C., Nielsen, C. H., Heissel, S., Højrup, P., Hansen, P. R., Gillings, N. & Kjaer, A., 2015, In : Journal of Labelled Compounds and Radiopharmaceuticals. 58, 6, p. 227-33

Research output: Contribution to journalJournal articleResearchpeer-review

Labeling
Tumors
Chelating Agents
Trastuzumab
1-(1,3-carboxypropyl)-4,7-carboxymethyl-1,4,7-triazacyclononane

Acyl-CoA-binding protein in the rat. Purification, binding characteristics, tissue concentrations and amino acid sequence

Knudsen, J., Hojrup, P., Hansen, H. O., Hansen, H. F. & Roepstorff, P., 1. Jan 1989, In : Biochemical Journal. 262, 2, p. 513-519 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Diazepam Binding Inhibitor
Purification
Mass spectrometry
Rats
Tissue

Agarose gel shift assay reveals that calreticulin favors substrates with a quaternary structure in solution

Boelt, S. G., Houen, G. & Højrup, P., 2015, In : Analytical Biochemistry. 481, p. 33-42

Research output: Contribution to journalJournal articleResearchpeer-review

Calreticulin
Sepharose
Assays
Gels
Substrates

Aluminum hydroxide adjuvant differentially activates the three complement pathways with major involvement of the alternative pathway

Güven, E., Duus, K., Laursen, I., Højrup, P. & Houen, G., 2013, In : PLOS ONE. 8, 9, p. e74445

Research output: Contribution to journalJournal articleResearchpeer-review

Aluminum Hydroxide
aluminum hydroxide
adjuvants
complement
Chemical activation

Amino acid exchange and covalent modification by cysteine and glutathione explain isoforms of fatty acid-binding protein occurring in bovine liver

Dörmann, P., Börchers, T., Korf, U., Højrup, P., Roepstorff, P. & Spener, F., 5. Aug 1993, In : Journal of Biological Chemistry. 268, 22, p. 16286-92 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Fatty Acid-Binding Proteins
Liver
Glutathione
Cysteine
Protein Isoforms

Amino acid sequence around the active-site serine residue in the acyltransferase domain of goat mammary fatty acid synthetase

Mikkelsen, J., Højrup, P., Rasmussen, M. M., Roepstorff, P. & Knudsen, J., 1. Apr 1985, In : The Biochemical journal. 227, 1, p. 21-7 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Acyltransferases
Fatty Acid Synthases
Serine
Catalytic Domain
Transferases

Amino acid sequence of acyl-CoA-binding protein from cow liver

Mikkelsen, J., Højrup, P., Nielsen, P. F., Roepstorff, P. & Knudsen, J., 1. Aug 1987, In : The Biochemical journal. 245, 3, p. 857-61 5 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Diazepam Binding Inhibitor
Fast Atom Bombardment Mass Spectrometry
Liver
Mass spectrometry
Gases

Amino acid sequence of bovine protein Z: a vitamin K-dependent serine protease homolog

Højrup, P., Jensen, M. S. & Petersen, T. E., 20. May 1985, In : FEBS letters. 184, 2, p. 333-8 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Vitamin K
Serine Proteases
Amino Acids
1-Carboxyglutamic Acid
Factor X

Amino acid sequence of the cold-active alkaline phosphatase from Atlantic cod (Gadus morhua).

Asgeirsson, B., Nielsen, B. N. & Højrup, P., 1. Sep 2003, In : Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology. 136, 1, p. 45-60 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Gadus morhua
Alkaline Phosphatase
Amino Acids
Gadiformes
Escherichia coli

Amino-acid sequence of the vitamin-K-dependent part of protein Z

Højrup, P., Roepstorff, P. & Petersen, T. E., Aug 1982, In : European Journal of Biochemistry. 126, 2, p. 343-8 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Vitamin K
Amino Acids
Peptides
1-Carboxyglutamic Acid
Pepsin A

Amnionless function is required for cubilin brush-border expression and intrinsic factor-cobalamin (vitamin B12) absorption in vivo

He, Q., Madsen, M., Kilkenney, A., Gregory, B., Christensen, E. I., Vorum, H., Højrup, P., Schäffer, A. A., Kirkness, E. F., Tanner, S. M., de la Chapelle, A., Giger, U., Moestrup, S. K. & Fyfe, J. C., 2005, In : Blood. 106, 4, p. 1447-1453

Research output: Contribution to journalJournal articleResearchpeer-review

Intrinsic Factor
Microvilli
Mutation
Dogs
Initiator Codon

An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins

Hägglund, P., Matthiesen, R., Elortza, F., Højrup, P., Roepstorff, P., Jensen, O. N. & Bunkenborg, J., 1. Aug 2007, In : Journal of Proteome Research. 6, 8, p. 3021-3031 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Glycosylation
Polysaccharides
Blood Proteins
Peptides
Glycoside Hydrolases

An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

Skottrup, P. D., López, R., Ksiazek, M., Højrup, P., Baelum, V., Potempa, J. & Kaczmarek, J. Z., Jun 2019, In : Journal of Immunological Methods. 469, p. 26-32

Research output: Contribution to journalJournal articleResearchpeer-review

Saliva
Immunoassay
Periodontitis
Point-of-Care Systems
Surface Plasmon Resonance

Applications of plasma desorption mass spectrometry in peptide and protein chemistry

Roepstorff, P., Nielsen, P. F., Klarskov, K. & Højrup, P., Oct 1988, In : Biomedical & environmental mass spectrometry. 16, 1-12, p. 9-18 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Peptides
Proteins
Protein Sequence Analysis
Molecular Weight

A proteome-scale study on in vivo protein N(α)-acetylation using an optimized method.

Zhang, X., Engholm-Keller, K. & Højrup, P., 2011, In : Proteomics. 11, p. 81-93 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A proteome-scale study on in vivo protein Nα-acetylation using an optimized method

Zhang, X., Ye, J., Engholm-Keller, K. & Højrup, P., 1. Jan 2011, In : Proteomics. 11, 1, p. 81-93 13 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Acetylation
Proteome
Peptides
Resins
Proteins

A proteomics approach to study in vivo protein N(alpha)-modifications.

Zhang, X., Ye, J. & Højrup, P., 2009, In : Journal of Proteomics. 73, 2, p. 240-251 12 p.

Research output: Contribution to journalJournal articleResearchpeer-review

A Selected Reaction Monitoring (SRM)-Based Method for Absolute Quantification of Aβ38, Aβ40, and Aβ42 in Cerebrospinal Fluid of Alzheimer's Disease Patients and Healthy Controls

Pannee, J., Portelius, E., Oppermann, M., Atkins, A., Hornshaw, M., Zegers, I., Højrup, P., Minthon, L., Hansson, O., Zetterberg, H., Blennow, K. & Gobom, J., 2013, In : Journal of Alzheimer's Disease. 33, 4, p. 1021-1032

Research output: Contribution to journalJournal articleResearchpeer-review

Cerebrospinal Fluid
Alzheimer Disease
Research
Clinical Trials
Peptides

A Single Rainbow Trout Cobalamin-binding Protein Stands in for Three Human Binders

Greibe, E. H., Fedosov, S., Sorensen, B. S., Højrup, P., Poulsen, S. S. & Nexo, E., 2012, In : Journal of Biological Chemistry. 287, 40, p. 33917-25 9 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Transcobalamins
Oncorhynchus mykiss
Binders
Trout
Intrinsic Factor

Assessing high affinity binding to HLA-DQ2.5 by a novel peptide library based approach

Jüse, U., Arntzen, M., Højrup, P., Fleckenstein, B. & Sollid, L. M., 1. Apr 2011, In : Bioorganic & Medicinal Chemistry. 19, 7, p. 2470-7 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Peptide Library
Libraries
Mass spectrometry
Search Engine
Peptides

Biochemical and ultrastructural studies of the C-type lectin bovine conglutinin

Andersen, O., Nielsen, E. H., Storgaard, P., Højrup, P., Friis, P., Leslie, G. & Svehag, S. E., 1. Nov 1992, In : Journal of Structural Biology. 109, 3, p. 201-7 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Hydroxylamine
N-Acetylneuraminic Acid
Collagenases
Gold
Electron Microscopy

Biochemical characterization of bovine plasma thrombin-activatable fibrinolysis inhibitor (TAFI)

Valnickova, Z., Thaysen-Andersen, M., Højrup, P., Christensen, T., Sanggaard, K. W., Kristensen, T. & Enghild, J. J., 2009, In : BMC Biochemistry. 10, p. 13

Research output: Contribution to journalJournal articleResearchpeer-review

Carboxypeptidase B2
Plasmas
Fibrinolysis
Proteins
Glycosylation

Biochemical evidence for heme linkage through esters with Asp93 and Glu241 in human eosinophil peroxidase: The ester with Asp93 is only partially formed in vivo

Oxvig, C., Thomsen, A., Overgaard, M., Sørensen, E., Højrup, P., Bjerrum, M., Gleich, G. & Sottrup-Jensen, L., 1999, In : Journal of Biological Chemistry. 274, p. 16953-16958

Research output: Contribution to journalJournal articleResearchpeer-review

Calibrating MALDI mass spectra without calibrants

Højrup, P., 2005, In : American Biotechnology Laboratory. 23, p. 16-17

Research output: Contribution to journalJournal articleResearch

Cannabidiol is an effective helper compound in combination with bacitracin to kill Gram-positive bacteria

Wassmann, C. S., Højrup, P. & Klitgaard, J. K., 19. Feb 2020, (Accepted/In press) In : Scientific Reports.

Research output: Contribution to journalJournal articleResearchpeer-review

Chaperone-client interactions between Hsp21 and client proteins monitored in solution by small angle X-ray scattering and captured by crosslinking mass spectrometry

Rutsdottir, G., I Rasmussen, M., Hojrup, P., Bernfur, K., Emanuelsson, C. & Söderberg, C. A. G., 2018, In : Proteins. 86, 1, p. 110-123

Research output: Contribution to journalJournal articleResearchpeer-review

X ray scattering
Crosslinking
Mass spectrometry
X-Rays
Small Heat-Shock Proteins

Characterisation of the 1st SSI purified MBL standard

Laursen, I., Højrup, P., Houen, G. & Christiansen, M., 2008, In : Clinica Chimica Acta. 395, 1-2, p. 159-161 2 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Mannose-Binding Lectin
Salt removal
Mannose-Binding Protein-Associated Serine Proteases
Plasma (human)
Proteins

Characterization of a glutathione S-transferase and a related glutathione-binding protein from gill of the blue mussel, Mytilus edulis

Højrup, P., Fitzpatrick, J., Krag, T. O. & Sheehan, D., 1. Jan 1995, In : Biochemical Journal. 305, 1, p. 145-150

Research output: Contribution to journalJournal articleResearch

Characterization of an extensin-modifying metalloprotease: N-terminal processing and substrate cleavage pattern of Pectobacterium carotovorum Prt1

Feng, T., Nyffenegger, C., Højrup, P., Vidal-Melgosa, S., Yan, K-P., Fangel, J. U., Meyer, A. S., Kirpekar, F., Willats, W. G. & Mikkelsen, J. D., 2014, In : Applied Microbiology and Biotechnology. 98, p. 10077-10089

Research output: Contribution to journalJournal articleResearchpeer-review

Characterization of different cell culture media for expression of recombinant antibodies in mammalian cells: Presence of endogeneous contaminating bovine antibodies

Rasmussen, L. K., Larsen, Y. B. & Højrup, P., 2005, In : Protein Expression and Purification. 41, p. 373-377

Research output: Contribution to journalJournal articleResearchpeer-review

Characterization of equine vitamin D–binding protein, development of an assay, and assessment of plasma concentrations of the protein in healthy horses and horses with gastrointestinal disease

Pihl, T. H., Jacobsen, S., Olsen, D. T., Højrup, P., Grosche, A., Freeman, D. E., Andersen, P. H. & Houen, G., 2017, In : American Journal of Veterinary Research. 78, 6, p. 718-728

Research output: Contribution to journalJournal articleResearchpeer-review

Gastrointestinal Diseases
digestive system diseases
Vitamins
Horses
vitamins
Mass spectrometry
Glycoproteins
Gels
Glycosylation
Proteins

Characterization of the oligomer structure of human recombinant mannan-binding lectin

Jensen, P. H., Weilguny, D., Matthiesen, F., McGuire, K. A., Shi, L. & Højrup, P., 2005, In : Journal of Biological Chemistry. 280, p. 11043-11051

Research output: Contribution to journalJournal articleResearchpeer-review