Personal profile
Curriculum
Personal data:
Address: Department of Biochemistry and Molecular Biology
University of Southern Denmark
Campusvej 55, DK-5230 Odense M
Contacts: [email protected]; phone: +45 6550 2371
Academic degrees:
1986: PhD in Molecular Biology, Odense University
1982: MSc, Molecular Biology (major) and Chemistry (minor), The University of Aarhus
Employments:
2008-present: Professor MSO (Department of Biochemistry and Molecular Biology, University of Southern Denmark)
1991-2008: Associate Professor, (Department of Molecular Biology, Odense University, now University of Southern Denmark)
1989-1990: Postdoctoral fellow (Department of Molecular Biology, Odense University)
1988: Temporary Associate Professor (Dept. of Molecular Biology, Odense University)
1986-1988: Postdoctoral fellow (Department of Molecular Biology, Odense University)
1985-1986: Postdoctoral fellow (Department of Molecular Biology, Univ. of Aarhus)
Current research:
PHs research focuses on protein structure and function. A primary focus is the development of new methods for determining protein three-dimensional structure and protein interaction by chemical cross-linking followed by mass spectrometric determination of linkages. Another topic is the structure and function of the endoplasmatic proteins, in particular calreticulin and calnexin. Furthermore, development of methods for fast analysis of glycopeptides by mass spectrometry.
Specific topics
• Three-dimensional structure of calreticulin (mono, di- and multimers)
• Fast glycosylation analysis of immunoglobulins
• Glycopeptide quantitation
• Glycosylation analysis of cancer markers
• De novo proteomics of fish mucus proteins
Administrative experience:
2006-present: Ph.d. study leader, Faculty of Science, SDU
Editorial and reviewing tasks:
Editor for Journal of Proteomics.
Member of committees for appointments of assoc. and full Professorships in Denmark. Reviewer of grant proposals (Austria and Switzerland). Referee for a number of journals.
Supervision:
1 postdocs, 6 PhD students, 23 MSc students (4 Novo scholars; 1 Oticon) and 7 BSc students.
Congress organization:
Co-organizer of five conferences in Denmark on Mass spectrometry (2003-2006).
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Collaborations and top research areas from the last five years
Research output
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Host cell protein quantitation by LC-MS. Experimental demonstration, qualification, and comparison of methods in USP 1132.1
Chrone, V. G., Blaszczyk, A. J., Zhang, D. H., Nielsen, S. B., Crawford, J., Schwämmle, V., Højrup, P., Kofoed, T., Peckham, N. & Mørtz, E., 15. Nov 2025, In: Journal of Pharmaceutical and Biomedical Analysis. 265, 12 p., 117051.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile31 Downloads (Pure) -
Native structure of the monoclonal therapeutic CD20 antibody ocrelizumab
Chrone, V. G., Jespersen, J. C., Asani, D. C., Trier, N. H., Ray, S., Berthias, F., Willemoës, M., Holm, A., Frederiksen, J. L., Houen, G. & Højrup, P., Sept 2025, In: Biochimica et Biophysica Acta - Proteins and Proteomics. 1873, 5, 11 p., 141084.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile6 Downloads (Pure) -
Analysis of Polypeptides by Amino Acid Analysis
Højrup, P., 2024, Peptide Antibodies. Humana Press, p. 71-82 (Methods in Molecular Biology, Vol. 2821).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research
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Characterization of Synthetic Peptides by Mass Spectrometry
Chrone, V. G., Lorentzen, A. & Højrup, P., 2024, Peptide Antibodies. Houen, G. (ed.). Humana Press, Vol. 2821. p. 83-89 (Methods in Molecular Biology, Vol. 2821).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research
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Epitope mapping of SARS-CoV-2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls
Larsen, D. N., Kaczmarek, J. Z., Palarasah, Y., Graversen, J. H. & Højrup, P., 1. Jul 2024, In: BBA Proteins and Proteomics. 1872, 4, 141011.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile126 Downloads (Pure)
Activities
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Cross-linking human protein disulfide isomerase: what to do with a thousand cross-links?
Højrup, P. (Lecturer)
21. Nov 2013Activity: Talks and presentations › Talks and presentations in private or public companies
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MONOCLONAL PROTEINASE 3 ANTIBODIES BINDING AT THE ACTIVE SITE
Højrup, P. (Lecturer)
13. May 2012Activity: Talks and presentations › Talks and presentations in private or public companies
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Proteomics in Nutritional Research: A 2-DE based study of diet induced changes in the human plasma proteome
Højrup, P. (Examiner)
11. Dec 2011Activity: Examination and external supervision › Internal examination
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Structure determination of human calreticulin by chemical cross-linking and mass spectrometry
Højrup, P. (Lecturer)
9. Dec 2011Activity: Talks and presentations › Talks and presentations in private or public companies
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Methods (Journal)
Højrup, P. (Peer reviewer)
21. Nov 2011Activity: Editorial work and peer review › Peer review of manuscripts › Research
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Erhvervs PhD - Development of a deep learning-assisted platform for confident and complete characterization of host cell proteins in complex biopharmaceuticals
Højrup, P. (Project participant)
01/09/2023 → 31/08/2026
Project: Research Councils
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Uddannelses- og forskningsministeriet - ErhvervsPhD Victor Guldmann Chrone - Development of a deep learning-assisted platform for confident and complete characterization of host cell proteins in complex biopharmaceuticals
Højrup, P. (Project participant)
01/09/2023 → 31/08/2026
Project: Research Councils
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Innovationsfonden - Erhvervs PhD Daniel Nyberg Larsen Development of MS based routine epitope mapping techniques
Højrup, P. (Project participant)
01/02/2020 → 31/01/2023
Project: Research
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The Michael J. Fox Foundation for Parkinson's Research - Alpha-synuclein oligomers, conformation specific MJF14 antibody and their interrelations. New applications, structure and functional analyses, attempts to obtain true "oligomer specific" conformational binders
Højrup, P. (Head coordinator)
01/01/2018 → 01/04/2020
Project: Research
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Uddannelses- og Forskningsministeriet – Innovationsfonden - Erhvervs phd Jakub Zbigniew Kaczmarek Early identification and inhibition of PPAD-associated rheumatold arthritis via novel lgy antibody development and scale-up
Højrup, P. (Head coordinator)
06/02/2017 → 05/02/2020
Project: Research
Press/Media
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Fra laboratorium i bruseniche til førende i medicinsk milliardniche
15/04/2025
1 Media contribution
Press/Media: Press / Media
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Fra laboratorie i bruser til førende i milliardindustri: Vi er jubelidioter
12/03/2025
1 Media contribution
Press/Media: Press / Media
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Alphalyse: For os har det kunne betale sig at være et par jubelidioter uden kapital eller udstyr
05/03/2025
1 Media contribution
Press/Media: Press / Media
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Internationalt gennembrud for mindre fynsk biotek-firma
04/03/2025
1 Media contribution
Press/Media: Press / Media
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Stort gennembrud for mindre fynsk firma: - Vi følte os lidt som prostituerede
03/03/2025
1 Media contribution
Press/Media: Press / Media