Curriculum

Name: Henrik Holbech
Adress: University of Southern Denmark, Institute of Biology.
Campusvej 55, 5230 Odense M
Tlf. (office): +45 6550 2770
Fax: +45 6550 2786
E-mail: [email protected]

Education:
Jun  2013 University Lecturer Training Programme, University of Southern Denmark 
Oct  2009 PhD in Ecotoxicology, University of Southern Denmark
Jan 1999 M.Sc. in Experimental Biology, University of Southern Denmark
Jun 1995 B.Sc., Biology, University of Southern Denmark

Employment
Jan 2015 - present - Associate Professor, Ecotoxicology, University of Southern Denmark
Oct 2009 - Dec 2014 - Post Doc, ecotoxicology, University of Southern Denmark
Jan 2001 - Oct 2009 - Academic staff, ecotoxicology, University of Southern Denmark 
Jan 2000 Research assistent, Ecotoxicology, University of Southern Denmark 

Funding and projects
Feb 2019 - Feb 2021 EU Tender project No 07.0203/2018/794670/ETU/ENV.B.2. Development of a study protocol for regulatory testing to identify endocrine disrupting substances in biotic systems (400,000 €). A collaboration between University of Heidelberg, University of Antwerp and University of Southern Denmark. Project leader Thomas Braunbeck 

Jan 2019 - Dec 2023 EU Horizon 2020 Grant Agreement number: 825753 — ERGO — H2020-SC1-BHC-2018-2020/H2020-SC1-2018-Single-Stage-RTD. Breaking down the wall between human health and environmental testing of endocrine disrupters: EndocRine Guideline Optimisation-ERGO (5,992,437 €) A EU Horizon 2020 RIA with 15 partners. Project Coordinator Henrik Holbech 

Jan 2017 - Dec 2020 Danish EPA Project J. No. 621-00212 (5.2 Mio DKK). Development and validation of ecotoxicological test methods for evaluation of endocrine disrupting properties of chemicals in preparation for development of OECD test guidelines. Project leader professor Poul Bjerregaard.

Jan 2015 - Dec 2015  NKG project number 2273. Development of endocrine endpoints for ED Test Guidelines in molluscs (350.000 DKK). A NordUTTE collaboration between Norway, Sweden and Denmark. Financed by Nordic council of minister. Project leader: Henrik Holbech 

Aug 2014 - Dec 2014 Danish EPA project J. No. 603-00133. Support to reply on comments in relation to the substance evaluation of benzophenone (50 000 DKK). Project leader: Henrik Holbech

Jan 2014 - Dec 2014 NKG project No. 2097. Development of endocrine endpoints for ED test guidelines in molluscs (350 000 DKK). A NordUTTE collaboration between Norway, Sweden and Denmark. Financed by Nordic council of minister. Project leader: Henrik Holbech

Jan 2013 - Dec 2013 Danish EPA Project J.no. 621-00140 (50 000 DKK) Evaluation of whether the phthalates DEHP, DBP, BBP and DIBP are endocrine disrupting chemicals. Project leader: Henrik Holbech

2013 Development of endocrine endpoints for ED test guidelines in molluscs (360 000 DKK). A NordUTTE collaboration between Norway, Sweden and Denmark. Financed by Nordic council of minister. Project leader: Henrik Holbech.

Jan 2013 - Dec 2016 Danish EPA Project J. No. 621-00066 (5.2 Mio DKK). Development and validation of ecotoxicological test methods for evaluation of endocrine disrupting properties of chemicals in preparation for development of OECD test guidelines. Project leader professor Poul Bjerregaard.

2012 NKG Project No. 1639. Development of endocrine endpoints for ED test guidelines in molluscs (460 000 DKK). A NordUTTE collaboration between Norway, Sweden and Denmark. Financed by Nordic council of minister. Project leader: Henrik Holbech.

2011 NKG Project No.1412 (100 000 DKK). Experimental development of the Fish Sexual Development Test (FSDT) - Finalization. Financed by Nordic council of minister. Project leader: Henrik Holbech.

Jan 2009 – Dec 2012 Projektet J.nr.M 7041-0049 (5.2 Mio DKK) ”Development and validation of fish tests: OECD test-guidelines to evaluate endocrine disrupting chemicals. A collaboration between University of Southern Denmark and DHI Hoersholm. Financed by the Danish Environmental Protection Agency. Project leader: Professor Poul Bjerregaard.

Jan 2005 – Dec 2008 Projektet J.nr.M 7041-0049 (5.2 Mio DKK) ”Development of test systems with zebrafish (Danio rerio) to evaluate the effects of endocrine disrupting chemicals with the purpose of creating an OECD test guideline”. A collaboration between University of Southern Denmark and DHI Hoersholm. Financed by the Danish Environmental Protection Agency. Project leader: Professor Poul Bjerregaard.

Jan 2001 – Dec 2004 Project ”Development of test systems with zebrafish (Danio rerio) to evaluate the effects of endocrine disrupting chemicals”. A collaboration between University of Southern Denmark and DHI Hoersholm. Finansed by the Danish Environmental Protection Agency. Project leader: Professor Poul Bjerregaard.

Member of
Jan 2012 - SETAC - Global Advisory Group on Endocrine Disruptor Testing and Risk Assessment (EDTRA)
Jan 2011 - Member of "OECD mollusc expert group"
Apr 2008 - Member of “OECD Expert group on the Zebrafish Embryo Toxicity Test (FET)”
Jan 2007 - Member of ”OECD Fish Drafting Group”
Jan 2007 - Member of OECD VMG-Eco (Validation Management Group - on ecotoxicity tests)
Jan 2000 - Member of the research network “Nordic zebrafish group (ZED group)”

Commenting and consultancy
2013 Commenting for the Danish EPA: Comments to BASF response to draft decision on triclosan substance evaluation
Report for the Danish EPA: Proposal for a testing strategy for endocrine disrupting chemicals.
Commenting for the Danish EPA: Danish comments to the Draft Fish Embryo Toxicity Test (FET)_v9

2012 Report for the Danish EPA: Evaluation of endocrine mediated effects of triclosan.
Commenting for the Danish EPA: commenting round on draft revised OECD TG 210 on Fish Early Life-Stage. Version 2012.
Commenting for the Danish EPA: Commenting on the draft GD on developing and assessing Adverse Outcome Pathways (AOP)

2011 Co-author "Report on Criteria for Endocrine Disrupters" for the Danish EPA via the Danish research program: “Center for hormonforstyrrende stoffer (CEHOS)”
Head author “OECD Fish Sexual Development Test Phase 2 validation report
Commenting for the Danish EPA: "Danish comments to the OECD Guidance Document on assessment of Endocrine Disrupting chemicals version 11
Commenting for the Danish EPA: "Danish comments to the OECD Guidance Document on assessment of Endocrine Disrupting chemicals version 9

2010 Commenting for the Danish EPA: “Danish comments to the OECD Fish Toxicity Testing Framework report version 2010"
Head author: “OECD Fish Sexual Development Test Phase 1 validation report”
Commenting for the Danish EPA: "Danish comments to the OECD Guidance Document on assessment of Endocrine Disrupting chemicals version 7
Commenting for the Danish EPA: “Danish comments to the OECD Fish Toxicity Testing Framework report version 2010"
Commenting for the Danish EPA: “Scientific report about endocrine disrupting chemicals in molluscs”
Commenting for the Danish EPA: "Danish comments on Detailed review paper on environmental endocrine disrupter screening: The use of receptor binding and transactivation assays in fish".

2009Commenting for the Danish EPA: “Danish reviewer of the OECD draft test guideline “androgenised female stickleback screening assay”
2008Commenting for the Danish EPA: "Danish comments on threshold approach for fish acute toxicity testing"
2007 Commenting for the Danish EPA: ”Danish Comments to Draft rev. DRP on Fish Long term Tests (2007)”
Commenting for the Danish EPA: ”Danish Comments to Draft Para tert butyl phenol FSA protocol (2007)”
Commenting for the Danish EPA: ”Danish Comments to the Ecotoxicological Risk Assessment of Prochloraz: Effects on aquatic organisms (2007)”
Commenting for the Danish EPA: ”Danish Comments to the OSPAR OECD Test Protocols for EDCs (2007)”
Commenting for the Danish EPA: ”Danish Comments to the Fish Life Cycle Toxicity Test revised Detailed Review Paper (2007)”

Konferences & Meetings 
2013 OECD expert meeting in the OECD mollusc expert group, Paris, 14-15 January
SETAC Europe 23 annual meeting, Glasgow, 11-16 May
Workshop on emerging organic pollutants, Toronto, 12-13 February
OECD expert meeting in the OECD mollusc expert group, Paris, 17-18 June

2012 OECD expert meeting in the OECD Mollusc expert group, Frankfurt, 19-20 September.
OECD expert meeting: Meeting in the Fish Embryo Toxicity test group, Berlin, 16-17 February.
Conference: Danish shipowner association ballast water treatment conference, Copenhagen, 8 March.
SETAC 6th World Congress : SETAC Europe 22nd Annual Meeting, Berlin, 20-24 May (posters and platforms).
6th SETAC Europe Special Science Symposium : Environmental Endocrine Disrupter testing and evaluation, Bruxelles, 24-25 October.
OECD expert meeting in the OECD Mollusc expert group, Frankfurt, 19-20 March.

2011 OECD Expert meeting: "8th meeting of the VMG-Eco", 9-10 November 2011
Nord-UTTE meeting: “Nordic NanoNet Workshop and EDC discussion” Hanasaari, Helsinki, Finland, 12-13 October 2011 (presentation).
The Conference: “SETAC Europe 21th Annual Meeting, Milan, Italy, 15. maj 2011 - 19. maj (posters)
OECD expert meeting: "23rd Meeting of the Working Group of National Co-ordinators of the Test Guidelines Programme (WNT)" 12-14 April, Paris, France
OECD expert meeting: "Second Meeting of the Advisory Group on Endocrine Disrupter Testing and Assessment (EDTA AG), 18-19 April
OECD expert meeting: "Fish Drafting Group meeting" 9-10 February, Tokyo, Japan

2010 OECD-workshop: "Fish testing framework workshop, 28-30 September Jealott’s hill, United Kingdom
OECD expert meeting: "Fish drafting group meeting" 16-17 June, Paris, France
The Conference: “SETAC Europe 20th Annual Meeting, Sevilla, Spain, 25. May 2010 – May 29th. (posters)

2009 The Conference: “SETAC Europe 19th Annual Meeting, Gothenburg, Sweden, May 29 – June 4th (posters)
OECD expert meeting, 7th meeting of the VMG-Eco, Paris, 8-9 December, Paris, France 
OECD-Workshop: "Workshop on Endocrine Disrupters Testing and Assessment (EDTA)" Copenhagen 22-24 September 2008

2008 Expert meeting of the Fish Drafting Group: 21-22 October 2008, OECD Headquarters, Paris, France.
Network meeting: The 7th NordUTTE Network meeting, Solbacka, Sweeden. 3-5 September 2008 (platform presentation)
Konference: “SETAC Europe 18th Annual Meeting, Warzawa, Polen, 25. maj 2008 - 29. maj (platform presentation)
6th MEETING OF THE VALIDATION MANAGEMENT GROUP FOR ECOTOXICITY testing Paris, 16-17 January 2008

2007 Konference: “SETAC Europe 17th Annual Meeting, nr. 17, Porto, Portugal, 20. May 2007 - 24. May 2007. (platform presentation)
5´th MEETING OF THE VALIDATION MANAGEMENT GROUP FOR ECOTOXICITY TESTING,

2006 Network meeting for PREDFISH, (“Predicting Chronic Effects in Fish from Sublethal Markers“) den 14-15/11 i Oslo (platform presentation).
Konference “Controversies and Solutions in Environmental Sciences. SETAC Europe 16´th annual meeting (SETAC) in Den Haag, Holland, 7-11 May (posters)

2005 Konference “ Europe 15´th annual meeting (SETAC) in Lille, Frankrig, 22-26 May (posters)

2004 Konference “Environmental Science Solutions: A Pan-European Perspective (SETAC) I Prag, Tjekkiet fra den 18-22 April (posters)
Project meeting in ”Project Group on Zebrafish Test Development”, under Nordic Council of Ministers, Prague, Czech republic, 21 April

2003 Konference „Kosmetika: Wirkungen und umweltverhalten von synthetischen parfümstoffen und UV-filtern“ i Zürich, schweitz 31 January (platform presentation)
Project meeting ”Project Group on Zebrafish Test Development”, Nordic council of Ministers. Stockholm 23 April and Uppsala 19 November.
Konference “Understanding the Complexity of Environmental Issues: A way to sustainability”, (SETAC) i Hamburg, Tyskland, 30 April-1 May (posters)

2002 Project meeting “Nordic Project Group on Zebrafish Test Development”, Åland, Finland, 4-5 February, Wien, Austria, 16 May and Copenhagen 9´th December.
Konference: Challenges in Environmental Risk Assessment and Modelling, (SETAC) in Vienna, Austria, 12-16 May (postere)
International Symposium on Endocrine Active Substances and Supplementary Workshop (SCOPE/IUPAC) in Yokohama, Japan, 15-23 November.
2nd Copenhagen Workshop on Endocrine Disrupters in Copenhagen 7-9 December

2001 EU-meeting: Expert meeting on the zebrafish test, Stockholm, 10 May 2001.
Project meeting ”Nordic Project group on zebrafish test development, under Nordic Council of Ministers ” Oslo 24 January, Hoersholm 25-26 June and Oslo 23 October.

2000 The workshop: Hormones and endocrine disrupters in food and water: Possible Impact on Human Health at Rigshospitalet, 27-30 May, 2000. The Fourth Network meeting on Development of Test Guidelines i Hurdalsjøen, Norway, 9-11 October.

Publications, peer-review 

Audouze, K., Zgheib, E., Abass, K., Baig A.H., Forner-Piquer, I., Holbech, H., Knapen, D., Leonards, P.E.G., Lupu, D.I.,Palaniswamy, S., Rautio, A., Sapounidou, M., Martin, O.V. 2021. Evidenced-Based Approaches to Support the Development of Endocrine-Mediated Adverse Outcome Pathways: Challenges and Opportunities. Frontiers in Toxicol. Dec 2021, Vol 3. DOI: 10.3389/ftox.2021.787017

Søgaard, R., Holbech, H., Nørum, U. & Bjerregaard, P. 2021. Investigation of the in vivo estrogenicity of the UV-filters 4-methylbenzylidene camphor and octyl methoxy cinnamate in rainbow trout (Oncorhynchus mykiss). Ecotoxicology and Environmental Safety. 224, 6 p., 112657. Doi: 10.1016/j.ecoenv.2021.112657.

Kernen, L., Rieder, J., Duus, A., Holbech, H., Segner, H., Bailey, C. 2020. Thymus development in the zebrafish (Danio rerio) from an ecoimmunology perspective. Journal of experimental zoology Part A. Volume: 333 Issue: 10 PP 805-819.

Knapen, D., Stinckens, E., Cavallin, J.E., Ankley, G.T., Holbech, H., Villeneuve, D.L., Vergauwen, L. 2020. Toward an AOP Network-Based Tiered Testing Strategy for the Assessment of Thyroid Hormone Disruption. Environmental Science & Technology. Volume: 54 Issue: 14 PP 8491-8499.DOI: 10.1021/acs.est.9b07205.   

Holbech, Henrik; Matthiessen, Peter; Hansen, Martin; Schuurmann, Gerrit; Knapen, Dries; Reuver, Marieke; Flamant, Frederic; Sachs, Laurent; Kloas, Werner; Hilscherova, Klara; Leonard, Marc; Arning, Jurgen; Strauss, Volker; Iguchi, Taisen; Baumann, Lisa. 2020. ERGO: Breaking Down the Wall between Human Health and Environmental Testing of Endocrine Disrupters. International journal of molecular sciences, Vol 21, Issue 8. DOI: 10.3390/ijms21082954. 

Andreasen, M., Lundgreen, K., Holbech, H., Hedegaard, M.A.B. 2020. Raman spectroscopy as a tool for viability assessment of planktonic organisms in UV treated ballast water. Vibrational Spectroscopy. Volume: 110, Article Number: 103142 DOI: 10.1016/j.vibspec.2020.103142

Baumann, L., Holbech, H., Schmidt-Posthaus, H., Moissl, A.R., Hennies, M., Tiedemann, J., Weltje, L., Segner, H., Braunbeck, T. 2020. Does hepatotoxicity interfere with endocrine activity in zebrafish (Danio rerio)? Chemosphere Vol 238. https://doi.org/10.1016/j.chemosphere.2019.124589.   

Lundgreen, K., Holbech, H., Pedersen, K. L., Petersen, G. I., Andreasen, R. R., George, C., Drillet, G., Andersen, M. 2018. UV fluences required for compliance with ballast water discharge standards using two approved methods for algal viability assessment. Marine Pollution Bulletin. Vol. 135, PP 1090-1100.

Ankley, G.T., Coady, K.K., Gross, M., Holbech, H., Levine, S.L., Maack, G., Williams, M. 2018. A Critical Review of the Environmental Occurrence and Potential Effects in Aquatic Vertebrates of the Potent Androgen Receptor Agonist 17beta-Trenbolone. Environmental toxicology and chemistry. DOI:10.1002/etc.4163

Bjerregaard, P., Kinnberg, K.L., Mose, M.P., Holbech, H. 2018. Investigation of the potential endocrine effect of nitrate in zebrafish Danio rerio and brown trout Salmo trutta. Comp. biochem. Physiol. Toxicology & pharmacology Vol:211 PP:32-40

Morthorst, J. E., Mathiesen, K. K., Holbech, H., Pedersen, K. L. & Bjerregaard, P. 2018. Vitellogenin concentrations in feral Danish brown trout have decreased: An effect of improved sewage treatment in rural areas? Environmental Toxicology and Chemistry. Vol: 37 Issue: 3 PP: 839-845

Coady, K.K., Biever, R.C., Denslow, N.D., Gross, M., Guiney, P.D., Holbech, H et al. 2017. Current limitations and recommendations to improve testing for the environmental assessment of endocrine active substances. Integrated Enviromental Assessment and Management. Vol 13. Issue 2. PP 302-316.

Peter Matthiessen, P., Ankley, G.T., Biever, R.C., Bjerregaard, P., Borgert, C., Brugger, K., Blankinship, A., Chambers, J., Coady, K.K., Constantine, L., Dang, Z., Denslow, N.D., Dreier, D.A., Dungey, S., Earl Gray, L., Gross, M., Guiney, P.D., Hecker, M., Holbech, H. et al. 2017. Recommended approaches to the scientific evaluation of ecotoxicological hazards and risks of endocrine-active substances Integrated Enviromental Assessment and Management. Vol 13. Issue 2. PP 267–279.

Charles, S., Ducrot, V., Azam, D., Benstead, R., Brettschneider, D., De Schamphelaere, K., Filipe Goncalves, S., Green, J.W., Holbech, H., Hutchinson, T.H., Faber, D., Laranjeiro, F., Matthiessen,  P., Norrgren, L., Oehlmann, J., Reategui-Zirena, E., Seeland-Fremer, A., Teigeler, M., Thome, J.P., Tobor Kaplon, M., Weltje, L., Lagadic, L. 2016. Optimizing the design of a reproduction toxicity test with the pond snail Lymnaea stagnalis. Reg. Toxicol. Pharmacol. 25;81:47-56. doi:  10.1016/j.yrtph.2016.07.012.

Örn, S., Holbech, H. & Norrgren, L. 2016: Sexual disruption in zebrafish (Danio rerio) exposed to mixtures of 17α-ethinylestradiol and 17β-trenbolone. Env. Toxicol. and Pharmacol. 41, 225-231

    
Kinnberg KL, Petersen GI, Albrektsen M, Minghlani M, Awad SM, Holbech BF, Green, J.W., Bjerregaard, P., Holbech, H. 2015. Endocrine-disrupting effect of the ultraviolet filter benzophenone-3 in zebrafish, Danio rerio. Environmental Toxicology and Chemistry. 2015;34(12):2833-2840.

    
Ducrot V, Askem C, Azam D, Brettschneider D, Brown R, Holbech, H et al. Development and validation of an OECD reproductive toxicity test guideline with the pond snail Lymnaea stagnalis (Mollusca, Gastropoda). Regulatory Toxicology and Pharmacology. 2014 dec;70(3): 605-614

Holbech, H., Schröder, K. D., Nielsen, L. M., Brande-Lavridsen, N., Holbech, B. F. & Bjerregaard, P. 19 Sep 2013. Estrogenic effect of the phytoestrogen biochanin A in zebrafish, Danio rerio, and brown trout, Salmo trutta. Aquatic Toxicology. 144-145, p. 19-25

Baumann, L., Holbech, H., Keiter, S., Kinnberg, K.L., Knörr, S., Nagel, T., Braunbeck, T. 2013. The maturity index as a tool to facilitate the interpretation of changes in vitellogenin production and sex ratio in the Fish Sexual Development Test. Aquatic Toxicology, Vol. 128– 129, p. 34– 42.

Keiter, S., Baumann, L., Farber, H., Holbech, H., Skutlarek, D., Engwall, M., Braunbeck, T. 2012. Long-term effects of a binary mixture of perfluorooctane sulfonate (PFOS) and bisphenol A (BPA) in zebrafish (Danio rerio). Aquatic Toxicology, Vol. 118-119, p. 116-129.

Holbech, H., Kinnberg, K.L., Brande-Lavridsen, N., Bjerregaard, P., Petersen, G.I., Norrgren, L., Örn, S., Braunbeck, T., Baumann, L., Bomke, C., Dorgerloh, M., Bruns, E., Ruehl-Fehlert, C., Green, J.W., Springer, T.A., Gourmelon, A. 2012. Comparison of zebrafish (Danio rerio) and fathead minnow (Pimephales promelas) as testspecies in the Fish Sexual Development Test (FSDT). Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology, Vol. 155, No. 2, p. 407-415.

Knudsen, J.J.G., Holbech, H., Madsen, S., Bjerregaard, P. 2011. Uptake of 17ß-estradiol and biomarker responses in brown trout (Salmo trutta) exposed to pulses. Environmental Pollution, Vol. 159, p. 3374-3380.

Morthorst, J.E., Holbech, H., Bjerregaard, P. 2010.Trenbolone causes irreversible masculinization of zebrafish at environmentally relevant concentrations. Aquatic Toxicology, Vol. 98, No. 4, p. 336-343.

Halden, A.N., Nyholm, J.R., Andersson, P.L., Holbech, H., Norrgren, L. 2010. Oral exposure of adult zebrafish (Danio rerio) to 2,4,6-tribromophenol affects reproduction. Aquatic Toxicology, Vol. 100, No. 1, p. 30-37.

T.F. Holtha., R. Nourizadeh-Lillabadic., M. Blaesbjerg., M. Grunga., H. Holbech., G.I. Petersen., P. Aleström and K. Hylland. 2008. Differential gene expression and biomarkers in zebrafish (Danio rerio) following exposure to produced water components. Aquatic Toxicology. 90. 277–291.
 
Bjerregaard, P, PR Hansen, KJ Larsen, C Erratico, B Korsgaard, H Holbech. 2008. Vitellogenin as a biomarker for estrogenic effects in brown trout, Salmo trutta: Laboratory and field investigations. Environ. Toxicol. Chem. 27 (11): 2387-2396. 

Kinnberg, K.; Holbech, H.; Petersen, G.I.; Bjerregaard, P. 2007. Effects of the fungicide prochloraz on the sexual development of zebrafish (Danio rerio). Comparative Biochemistry and Physiology C. 145, 165-170.
 
Holbech, H.; Kinnberg, K.; Petersen, G.I.; Jackson, P.; Hylland, K.; Norrgren, L.; Bjerregaard, P. 2006. Detection of endocrine disrupters: Evaluation of a Fish Sexual Development Test (FSDT). Comparative Biochemistry and Physiology C. 144, 57-66. 

Örn, S., Svenson, A., Viktor, T., Holbech, H., Norrgren, L. 2006. Male-biased sex ratios and vitellogenin induction in zebrafish exposed to effluent water from a Swedish pulp mill. Arch. Environ. Contam. Toxicol. 51. 445-451. 

Andersen, L., Kinnberg, K., Holbech, H., Korsgaard, B., Bjerregaard, P. 2005. Evaluation of a 40 day assay for testing endocrine disrupters: effects of an anti-estrogen and an aromatase inhibitor on sex ratio and vitellogenin concentrations in juvenile zebrafish (Danio rerio). Fish Physiology and Biochemistry. (2004) 30: 257-266. 

Andersen, L., Holbech, H., Gessbo, Å., Norrgren L., Petersen, G.I. 2003. Effects of exposure to 17α-ethinylestradiol during early development on sexual differentiation and induction of vitellogenin in Zebrafish (Danio rerio). Comparative Biochemistry and Physiology C 134, 365-374. 

Andersen, L., Petersen, G. I., Gessbo, Å., Örn, S., Holbech, H., Bjerregaard, P., Norrgren L. 2001. Zebrafish Danio rerio and roach Rutilus rutilus: Two species suitable for evaluating effects of endocrine disrupting chemicals. Aquatic Ecosystem Health and Management, 4, 275-282. 

Holbech, H., Andersen, L., Petersen, G. I., Korsgaard, B., Pedersen, K. L., Bjerregaard, P. 2001. Development of an ELISA for Vitellogenin in whole body homogenate of zebrafish (Danio rerio). Comp. Biochem. Physiol. C. 130 (1) 119-131. 

Holbech, H., Nørum, U., Korsgaard, B., Bjerregaard, P. 2002. The chemical UV-filter 3-benzylidene camphor causes an oestrogenic effect in an in vivo fish assay. Pharmacology & Toxicology, 91, 204-208. 

Orn, S., Holbech, H., Madsen, T.H., Norrgren, L., Petersen, G.I. 2003. Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone. Aquatic Toxicol. 65 (4): 397-411 

Rose, J., Holbech, H., Lindholst, C., Nørum, U., Povlsen, A., Korsgaard, B., Bjerregaard, P. 2002. Vitellogenin induction by 17-estradiol and 17a-ethinylestradiol in male zebrafish (Danio rerio). Comp. Biochem. Physiol. C 131, 531-539. 

Van der Ven, L.T.M., Holbech, H., Fenske M., Van den Brandhof J-E., Gielis-Proper, F.K., Wester P.W. 2003. Vitellogenin expression in zebrafish Danio rerio: evaluation by histochemistry, immunohistochemistry, and in situ mRNA hybridisation. Aquatic Toxicol. 65, 1-11.

Non peer review:
Holbech, H., Petersen, G.I., Norman, A., Örn, S., Norrgren, L., Bjerregaard, P. Comparison of vitellogenin concentrations in plasma and whole body homogenate from zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) TemaNord 2001:597, pp 48-51. Nordic Council of Ministers, Copenhagen, 2001. 

Norman, A., Örn, S., Holbech, H., Gessbo, Å., Parkkonen, J., Förlin, L., Norrgren, L. Exposure of zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) to 17α-ethinylestradiol and effluent from a sewage treatment plant. TemaNord 2001:597, pp 35-41. Nordic Council of Ministers, Copenhagen, 2001. 

Norrgren, L., Förlin, L., Holbech, H., Valle, M., and Örn. Zebrafish (Danio rerio) - A model species for evaluation of endocrine disrupting chemicals. TemaNord 2001:514, pp 27-28. Nordic Council of Ministers, Copenhagen, 2001. 

Örn, S., Norman, A., Holbech, H., Gessbo, Å., Petersen, G. I., Norrgren, L. Short-term exposure of zebrafish to pulp mill effluent and 17α-ethinylestradiol: A comparison between juvenile and adult fish. TemaNord 2001:597, pp 26-34. Nordic Council of Ministers, Copenhagen, 2001.
 
Petersen, G.I., Andersen, L., Holbech, H., and Norrgren, L. Sex differentiation and vitellogenin induction in zebrafish (Danio rerio) after early life stage exposure. TemaNord 2001:514, pp 18-25. Nordic Council of Ministers, Copenhagen, 2001. 

Petersen, G.I., Madsen, T., Holbech, H., and Norrgren, L. Partial life cycle test - evaluation of a 40-day juvenile zebrafish (Danio rerio) assay. TemaNord 2001:597, pp 14-25. Nordic Council of Ministers, Copenhagen, 2001. 

Valle, M., Örn, S., Holbech, H., Gessbo, Å, Norrgren, L. Oral exposure in zebrafish; An alternative route of exposure to endocrine disrupting chemicals. TemaNord 2001:597, pp 42-47. Nordic Council of Ministers, Copenhagen, 2001

Research areas

My main research area is about the development of OECD test guidelines using fish and invertebrates for the detection of endocrine disrupters. I am employed until Dec. 2016 in a project financed by the Danish EPA where we evaluate molluscs species as candidates to be test organisms in different OECD guideline drafts. I am leading a Nordic research group funded by the Nordic Council of Ministers. The group coordinates the Nordic work on guidelines for detection of endocrine disrupters in fish and invertebrates. I am a member of the OECD Validation Management Group for Ecotoxicology (VMG-ECO) and the OECD Fish Drafting Group (FDG) as well as the OECD expert group on Fish Embryo Toxicity Test (FET) and the OECD mollusc expert group. Since 2013 I have been member of the European Chemical Agency (ECHA)Endocrine disrupter Expert group. In relation to basic research I am very interested in invertebrate endocrinology and especially mollusc endocrinology where there are many black boxes to be investigated. I am also involved in a new research area dealing with ballast water treatment and assessment. We try to validate new methods to verify the life/death of organisms treated with different techniques as UV-radiation, cavitation and ozonation that are used to remove potential invasive species from ships ballast water. In the laboratory I mostly work with fish and invertebrate experiments and the development and maintenance of immunochemical methods as e.g. ELISA (enzyme linked immunosorbent assay).

Research information

Aquatic ecotoxicologist specialised in the development of OECD test guidelines for the detection of endocrine disrupting chemicals in fish and invertebrates. Research in mollusc endocrinology. Funding: Jan 2014 - Dec 2014 NordUTTE project 2097: Development of endocrine endpoints for ED Test Guidelines for in Molluscs. Project leader Henrik Holbech (350.000 DKK). Jan 2013-Dec 2013 NordUTTE project 1916: Development of endocrine endpoints for ED Test Guidelines for in Molluscs. Project leader Henrik Holbech (360.000 DKK). Jan 2013 - Dec 2016 Danish EPA Project J. No. 621-00066 (5.2 mio DKK). Development and validation of ecotoxicological test methods for evaluation of endocrine disrupting properties of chemicals in preparation for development of OECD test guidelines. Project leader professor Poul Bjerregaard. Jan 2009 - Dec 2012 Danish EPA Project J.No. 621-00048 (5.2 mio DKK). Development and validation of fish tests: OECD test guidelines for evaluation of endocrine disrupting properties of chemicals. A collaboration between SDU, Odense and DHI Hoersholm. Project leader Professor Poul Bjerregaard. Jan 2005 - Dec 2008 Danish EPA Project J.No. M 7041-0049 (5,2 mio) Development  of test methods for evaluation of endocrine disrupting properties of chemicals in fish with special emphasis on zebrafish (Danio rerio) in preparation for development of OECD test guidelines.A collaboration between SDU, Odense and DHI Hoersholm. Project leader Professor Poul Bjerregaard. Jan 2001 – Dec 2004 Danish EPA Project (4.8 mio DKK). Development of test systems with zebrafish (Danio rerio) for evaluation of endocrine disrupting properties of chemicals. A collaboration between SDU, Odense and DHI Hørsholm. Project leader Professor Poul Bjerregaard.