• Campusvej 55

    5230 Odense M

    Denmark

19952020
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Research Output 1995 2020

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Journal article
2020

Binding and intracellular transport of 25-hydroxycholesterol by Niemann-Pick C2 protein

Petersen, D., Reinholdt, P., Szomek, M., Hansen, S. K., Poongavanam, V., Juhl, A. D., Heegaard, C. W., Krishnan, K., Fujiwara, H., Covey, D. F., Ory, D. S., Kongsted, J. & Wüstner, D., 1. Feb 2020, In : B B A - Biomembranes. 1862, 2, 14 p., 183063.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Proteins
Sterols
Fluorescence
Fluorescence Recovery After Photobleaching
2019

Computational Characterization of a Cholesterol-Based Molecular Rotor in Lipid Membranes

Reinholdt, P., Wind, S., Wüstner, D. & Kongsted, J., 2019, In : Journal of Physical Chemistry B. 123, 34, p. 7313-7326 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
cholesterol
Membrane Lipids
rotors
lipids
13 Downloads (Pure)

Computational Modeling Explains the Multi Sterol Ligand Specificity of the N-Terminal Domain of Niemann-Pick C1-Like 1 Protein

Poongavanam, V., Kongsted, J. & Wüstner, D., 2019, In : ACS Omega. 4, 25, p. 20894-20904 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Cholesterol
Sterols
Ligands
Proteins
Molecular mechanics

Photophysical investigation of two emissive nucleosides exhibiting gigantic stokes shifts

Stendevad, J., Hornum, M., Wüstner, D. & Kongsted, J., 17. Jun 2019, In : Photochemical and Photobiological Sciences. 18, 7, p. 1858-1865 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Deoxycytidine
nucleosides
Nucleosides
analogs
shift

Rational design of novel fluorescent analogues of cholesterol: a "step-by-step" computational study

Bonvicini, A., Reinholdt, P., Tognetti, V., Joubert, L., Wüstner, D. & Kongsted, J., 24. Jun 2019, In : Physical Chemistry Chemical Physics. 21, 28, p. 15487-15503 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

multiphoton absorption
cholesterol
Cholesterol
analogs
membranes
61 Downloads (Pure)

Steady state analysis of influx and transbilayer distribution of ergosterol in the yeast plasma membrane

Wüstner, D., 2019, In : Theoretical Biology and Medical Modelling. 16, 26 p., 13.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Steady-state Analysis
Plasma Membrane
Cell membranes
Yeast
Cell Membrane

Structural Insight into Eukaryotic Sterol Transport through Niemann-Pick Type C Proteins

Winkler, M. B. L., Kidmose, R. T., Szomek, M., Thaysen, K., Rawson, S., Muench, S. P., Wüstner, D. & Pedersen, B. P., 3. Oct 2019, In : Cell. 179, 2, p. 485-497.e18

Research output: Contribution to journalJournal articleResearchpeer-review

Sterols
Protein C
Membranes
Tunnels
Protons
2018
124 Downloads (Pure)

A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching

Hansen, C. V., Schroll, A. & Wüstner, D., 23. Jan 2018, In : Scientific Reports. 8, 13 p., 1387.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fluorescence
Green Fluorescent Proteins
Microscopy
109 Downloads (Pure)

A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates

Lorenzen, C. V., Schroll, A. & Wüstner, D., 29. Nov 2018, In : B M C Biophysics. 11, 14 p., 7.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fluorescence
Proteins
Inclusion Bodies
Protein Aggregates
Cell Nucleus Active Transport

Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy

Dupont, A., Lund, F. W., Jensen, M. L. V., Szomek, M., Nielsen, G. K., Heegaard, C. W., Guttmann, P., Werner, S., McNally, J., Schneider, G., Kapishnikov, S. & Wüstner, D., 6. Nov 2018, In : BioRxiv.

Research output: Contribution to journalJournal articleResearch

111 Downloads (Pure)

Bayesian model selection with fractional Brownian motion

Krog, J. F. C., Hervig Jacobsen, L., Lund, F. W., Wüstner, D. & Lomholt, M. A., 18. Sep 2018, In : Journal of Statistical Mechanics: Theory and Experiment. 2018, 9, 23 p., 093501.

Research output: Contribution to journalJournal articleResearchpeer-review

File
Bayesian Model Selection
Fractional Brownian Motion
trajectories
Trajectory
goodness of fit
111 Downloads (Pure)

Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane

Solanko, L. M., Sullivan, D. P., Sere, Y. Y., Szomek, M., Lunding, A., Solanko, K. A., Pizovic, A., Stanchev, L. D., Pomorski, T. G., Menon, A. K. & Wüstner, D., 2018, In : Traffic. 19, 3, p. 198-214

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Ergosterol
Cell membranes
Yeast
Cell Membrane
Sterols

Live-cell imaging of new polyene sterols for improved analysis of intracellular cholesterol transport

Modzel, M., Solanko, K. A., Szomek, M., Hansen, S. K., Dupont, A., Nåbo, L. J., Kongsted, J. & Wüstner, D., 2018, In : Journal of Microscopy. 271, 1, p. 36-48

Research output: Contribution to journalJournal articleResearchpeer-review

Polyenes
Filipin
Membranes
Fluorescence Microscopy
Microscopy
133 Downloads (Pure)

Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts

Berzina, Z., Solanko, L. M., Mehadi, A. S., Jensen, M. L. V., Lund, F. W., Modzel, M., Szomek, M., Solanko, K. A., Dupont, A., Nielsen, G. K., Heegaard, C. W., Ejsing, C. S. & Wüstner, D., 2018, In : Chemistry and Physics of Lipids. 213, p. 48-61

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Sterols
Fibroblasts
Cell membranes
Cell Membrane
Cholesterol
134 Downloads (Pure)

Structural design of intrinsically fluorescent oxysterols

Nåbo, L. J., Modzel, M., Krishnan, K., Covey, D. F., Fujiwara, H., Ory, D. S., Szomek, M., Khandelia, H., Wüstner, D. & Kongsted, J., 2018, In : Chemistry and Physics of Lipids. 212, p. 26–34

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Structural design
Photons
Membranes
Fluorescence microscopy
Electronic structure
2016
150 Downloads (Pure)

A comparative study on fluorescent cholesterol analogs as versatile cellular reporters

Sezgin, E., Betul Can, F., Schneider, F., Clausen, M. P., Galiani, S., Stanly, T. A., Waithe, D., Colaco, A., Honigmann, A., Wüstner, D., Platt, F. & Eggeling, C., 2016, In : Journal of Lipid Research. 57, p. 299-309

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Cholesterol
Membranes
Assays
Cell membranes
Fluorescence

Computational analysis of sterol ligand specificity of the Niemann Pick C2 protein

Poongavanam, V., Kongsted, J. & Wüstner, D., 2016, In : Biochemistry. 55, 36, p. 5165-5179

Research output: Contribution to journalJournal articleResearchpeer-review

152 Downloads (Pure)

Embedding beyond electrostatics: The role of wave function confinement

Nåbo, L. J., Olsen, J. M. H., Holmgaard List, N., Solanko, L. M., Wüstner, D. & Kongsted, J., 14. Sep 2016, In : The Journal of Chemical Physics. 145, 7 p., 104102.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Wave functions
embedding
Electrostatics
wave functions
electrostatics

Imaging approaches for analysis of cholesterol distribution and dynamics in the plasma membrane

Wüstner, D., Modzel, M., Lund, F. W. & Lomholt, M. A., 2016, In : Chemistry and Physics of Lipids. 199, p. 106-135

Research output: Contribution to journalJournal articleResearchpeer-review

Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells

Wüstner, D., Lund, F. W., Röhrl, C. & Stangl, H., 2016, In : Chemistry and Physics of Lipids. 194, p. 12-28

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Cells
Fluorescence
Sterols
Cell membranes
2015

Computational Modeling of Fluorescence Loss in Photobleaching

Hansen, C. V., Schroll, A. & Wüstner, D., Aug 2015, In : Computing and Visualization in Science. 17, 4, p. 151-166

Research output: Contribution to journalJournal articleResearchpeer-review

Photobleaching
Computational Modeling
Fluorescence
Microscopy
Cell

Design of new fluorescent Cholesterol and Ergosterol analogs: Insights from theory

Nåbo, L. J., List, N. H., Witzke, S., Wüstner, D., Khandelia, H. & Kongsted, J., 2015, In : BBA General Subjects. 1848, 10, part A, p. 2188–2199

Research output: Contribution to journalJournal articleResearchpeer-review

2014

Atomistic Monte Carlo simulation of lipid membranes

Wüstner, D. & Sklenar, H., 24. Jan 2014, In : International Journal of Molecular Sciences. 15, 2, p. 1767-803 37 p., ijms15021767.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
lipids
membranes
Monte Carlo method
simulation
closures

Dehydroergosterol as an analogue for cholesterol: Why it mimics cholesterol so well?

Pourmosa, M., Rog, T., Mikkeli, R., Vattulaien, I., Solanko, L. M., Wüstner, D., List, N. H., Kongsted, J. & Karttunen, M., 2014, In : Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical. 118, p. 7345

Research output: Contribution to journalJournal articleResearchpeer-review

Photobleaching kinetics and time-integrated emission of fluorescent probes in cellular membranes.

Wüstner, D., Christensen, T., Solanko, L. M. & Sage, D., 29. Jul 2014, In : Molecules. 19, 8, p. 11096-11130 35 p., molecules190811096.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Photobleaching
Bleaching
Fluorescent Dyes
Membranes
Kinetics

SpatTrack: an imaging toolbox for analysis of vesicle motility and distribution in living cells.

Lund, F. W., Jensen, M. L., Christensen, T., Nielsen, G. K., Heegaard, C. W. & Wüstner, D., 16. Oct 2014, In : Traffic. 15, 12, p. 1406-29 25 p., tra.12228.

Research output: Contribution to journalJournal articleResearchpeer-review

Cells
Imaging techniques
Spatial Analysis
Fluorescence microscopy
Complex networks

The second-order polarization propagator approximation (SOPPA) method coupled to the polaizable continuum model

Eriksen, J. J., Solanko, L. M., Nåbo, L. J., Wüstner, D., Sauer, S. P. A. & Kongsted, J., 2014, In : Computational and Theoretical Chemistry. 1040-1041, p. 54

Research output: Contribution to journalJournal articleResearchpeer-review

2013

A comparison of single particle tracking and temporal image correlation spectroscopy for quantitative analysis of endosome motility

Lund, F. W. & Wustner, D., 2013, In : Journal of Microscopy. 252, 2, p. 169-188

Research output: Contribution to journalJournal articleResearchpeer-review

Noise
Signal-To-Noise Ratio
Lighting
Fibroblasts
Fluorescence

Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure

Solanko, L. M., Wüstner, D., Lund, F. W., Brewer, J. R., Midtiby, H., Bittman, R., Eggeling, C., Honigmann, A. & Dekaris, V., 5. Nov 2013, In : Biophysical Journal. 105, 9, p. 2082-2092 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Membranes
Cell membranes
Fluorophores
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
2012

Following Intracellular Cholesterol Transport by Linear and Non-Linear Optical Microscopy of Intrinsically Fluorescent Sterols

Wustner, D., 2012, In : Current Pharmaceutical Biotechnology. 13, 2, p. 303-318 16 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation

Wüstner, D., Solanko, L. M., Lund, F. W., Sage, D., Schroll, A. & Lomholt, M. A., 2012, In : B M C Bioinformatics. 13, p. 296

Research output: Contribution to journalJournal articleResearchpeer-review

Two-photon time-lapse microscopy of BODIPY-cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells

Lund, F. W., Lomholt, M. A., Solanko, L. M., Bittman, R. & Wustner, D., 2012, In : B M C Biophysics. 5, p. 20

Research output: Contribution to journalJournal articleResearchpeer-review

2011

Multicolor bleach-rate imaging enlightens in vivo sterol transport

Wüstner, D. & Sage, D., 2011, In : Communicative & Integrative Biology. 3, 4, p. 370-373 4 p.

Research output: Contribution to journalJournal articleResearchpeer-review

sterols
image analysis
bleaching
endosomes
Caenorhabditis elegans

Potential of ultraviolet widefield imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes

Wüstner, D., Brewer, J. R., Bagatolli, L. & Sage, D., Jan 2011, In : Microscopy Research and Technique. 74, 1, p. 92-108 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Microscopy
Microscopic examination
membranes
microscopy
Membranes

Quantitative assessment of sterol traffic in living cells by dual labeling with dehydroergosterol and BODIPY-cholesterol

Wustner, D., Solanko, L., Sokol, O., Garvik, O., Li, Z. G., Bittman, R., Korte, T. & Herrmann, A., 2011, In : Chemistry and Physics of Lipids. 164, 3, p. 221-235 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast: dehydroergosterol fluorescence study.

Kohut, P., Wüstner, D., Hronska, L., Kuchler, K., Hapala, I. & Valachovic, M., 2011, In : Biochemical and Biophysical Research Communications. 404, 1, p. 233-238 6 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Sterols
Yeast
Adenosine Triphosphate
Fluorescence
Ergosterol
2010

Selective visualization of fluorescent sterols in Caenorhabditis elegans by bleach-rate-based image segmentation

Wüstner, D., Landt Larsen, A., Færgeman, N. J., Brewer, J. R. & Sage, D., 1. Apr 2010, In : Traffic. 11, 4, p. 440-54 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Caenorhabditis elegans
Sterols
Image segmentation
Visualization
Bleaching
2009

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

Benninger, R. K. P., Vanherberghen, B., Young, S., Taner, S. B., Culley, F. J., Schnyder, T., Neil, M. A. A., Wüstner, D., French, P. M. W., Davis, D. M. & Onfelt, B., 1. Jan 2009, In : Biophysical Journal. 96, 2, p. L13-5

Research output: Contribution to journalJournal articleResearchpeer-review

Membranes
Cell Membrane
Optical Imaging
Photons
2008

Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets

Wüstner, D. & Faergeman, N. J., 2008, In : Cytometry. Part A. 73, 8, p. 727-744 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Color
Adipocytes
Foam Cells
Signal-To-Noise Ratio
Fluorescence Microscopy

Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages

Wüstner, D., 1. Aug 2008, In : Chemistry and Physics of Lipids. 154, 2, p. 129-136 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Macrophages
Sterols
Cell membranes
Phase separation
Cholesterol

Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells

Hartwig Petersen, N., Færgeman, N. J., Yu, L. & Wüstner, D., 2008, In : Journal of Lipid Research. 49, p. 2023 - 2037

Research output: Contribution to journalJournal articleResearchpeer-review

Sterols
Cell membranes
Recycling
Hepatocellular Carcinoma
Cell Membrane

Selective visualization of fluorescent sterols in Caenorhabditis elegans

Wüstner, D., Larsen, A. L., Brewer, J. R., Sage, D. & Færgeman, N. J., 2008, In : Chemistry and Physics of Lipids. 154, p. S42-S42 1 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells

Wüstner, D., Brewer, J. R., Fyhn, H. M. & Færgeman, N. J., 2008, In : Chemistry and Physics of Lipids. 154, Suppl. 1, p. S63-S63 1 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells

Wüstner, D. & Faergeman, N. J., 1. Nov 2008, In : Histochemistry and Cell Biology. 130, 5, p. 891-908 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Spatio-Temporal Analysis
endocytosis
Endocytosis
sterols
Membranes
2007

Plasma membrane sterol distribution resembles the surface topography of living cells

Wüstner, D., 1. Jan 2007, In : Molecular Biology of the Cell. 18, 1, p. 211-228 18 p.

Research output: Contribution to journalJournal articleResearchpeer-review

2006

Quantification of polarized traffiking of transferrin and comparison with bulk membrane transport in hepatic cells

Wüstner, D., 2006, In : Biochemical Journal. 400, p. 267-280

Research output: Contribution to journalJournal articleResearchpeer-review

Steady state analysis and experimental validation of a model for hepatic high density lipoprotein transport

Wüstner, D., 2006, In : Traffic. 7, p. 699-715

Research output: Contribution to journalJournal articleResearchpeer-review

Using internal and collective variables in Monte Carlo simulations of nucleic acid structures: chain breakage/closure algorihm and associated Jacobians

Sklenar, H., Wüstner, D. & Rohs, R., 2006, In : Journal of Computational Chemistry. 27, p. 309-315

Research output: Contribution to journalJournal articleResearchpeer-review

2005

Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells

Wüstner, D., Mondal, M., Tabas, I. & Maxfield, F. R., 1. May 2005, In : Traffic. 6, 5, p. 396-412

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Foam Cells
Macrophages
Sterols
Foams
Observation

Improved visualization and quantitative analysis of fluorescent membrane sterol in polarized hepatic cells

Wüstner, D., Oct 2005, In : Journal of Microscopy. 220, 1, p. 47-64

Research output: Contribution to journalJournal articleResearchpeer-review

Hepatocytes
Membranes
Recycling
dehydroergosterol
Cholesterol Esters