• Campusvej 55

    5230 Odense M

    Denmark

19952020
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Research Output 1995 2020

154 Downloads (Pure)

A comparative study on fluorescent cholesterol analogs as versatile cellular reporters

Sezgin, E., Betul Can, F., Schneider, F., Clausen, M. P., Galiani, S., Stanly, T. A., Waithe, D., Colaco, A., Honigmann, A., Wüstner, D., Platt, F. & Eggeling, C., 2016, In : Journal of Lipid Research. 57, p. 299-309

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Cholesterol
Membranes
Assays
Cell membranes
Fluorescence

A comparison of single particle tracking and temporal image correlation spectroscopy for quantitative analysis of endosome motility

Lund, F. W. & Wustner, D., 2013, In : Journal of Microscopy. 252, 2, p. 169-188

Research output: Contribution to journalJournal articleResearchpeer-review

Noise
Signal-To-Noise Ratio
Lighting
Fibroblasts
Fluorescence
124 Downloads (Pure)

A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching

Hansen, C. V., Schroll, A. & Wüstner, D., 23. Jan 2018, In : Scientific Reports. 8, 13 p., 1387.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fluorescence
Green Fluorescent Proteins
Microscopy
109 Downloads (Pure)

A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates

Lorenzen, C. V., Schroll, A. & Wüstner, D., 29. Nov 2018, In : B M C Biophysics. 11, 14 p., 7.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Fluorescence
Proteins
Inclusion Bodies
Protein Aggregates
Cell Nucleus Active Transport

Aminophospholipids have no access to the luminal side of the biliary canaliculus: Implications for the specific lipid composition of the bile fluid

Tannert, A., Wüstnert, D., Bechstein, J., Müller, P., Devaux, P. F. & Herrmann, A., 17. Oct 2003, In : Journal of Biological Chemistry. 278, 42, p. 40631-40639

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Phospholipid Transfer Proteins
Phosphatidylcholines
Lipids
Fluids
Labeling

Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy

Dupont, A., Lund, F. W., Jensen, M. L. V., Szomek, M., Nielsen, G. K., Heegaard, C. W., Guttmann, P., Werner, S., McNally, J., Schneider, G., Kapishnikov, S. & Wüstner, D., 6. Nov 2018, In : BioRxiv.

Research output: Contribution to journalJournal articleResearch

Analysis of Cholesterol Trafficking with Fluorescent Probes

Maxfield, F. R. & Wustner, D., 2012, LIPIDS, VOL 108. DiPaolo, G. & Wenk, MR. (eds.). Vol. 108. p. 367-393 27 p. (Methods in Cell Biology, Vol. 108).

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Atomistic Monte Carlo simulation of lipid membranes

Wüstner, D. & Sklenar, H., 24. Jan 2014, In : International Journal of Molecular Sciences. 15, 2, p. 1767-803 37 p., ijms15021767.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
lipids
membranes
Monte Carlo method
simulation
closures
111 Downloads (Pure)

Bayesian model selection with fractional Brownian motion

Krog, J. F. C., Hervig Jacobsen, L., Lund, F. W., Wüstner, D. & Lomholt, M. A., 18. Sep 2018, In : Journal of Statistical Mechanics: Theory and Experiment. 2018, 9, 23 p., 093501.

Research output: Contribution to journalJournal articleResearchpeer-review

File
Bayesian Model Selection
Fractional Brownian Motion
trajectories
Trajectory
goodness of fit

Binding and intracellular transport of 25-hydroxycholesterol by Niemann-Pick C2 protein

Petersen, D., Reinholdt, P., Szomek, M., Hansen, S. K., Poongavanam, V., Juhl, A. D., Heegaard, C. W., Krishnan, K., Fujiwara, H., Covey, D. F., Ory, D. S., Kongsted, J. & Wüstner, D., 1. Feb 2020, In : B B A - Biomembranes. 1862, 2, 14 p., 183063.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Proteins
Sterols
Fluorescence
Fluorescence Recovery After Photobleaching

Cholesterol trafficking and distribution between cellular membranes

Wüstner, D., Solanko, L. M. & Lund, F. W., 2012, Cholesterol Regulation of Ion Channels and Receptors . Levitan, I. & Barrantes, F. (eds.). John Wiley & Sons Ltd, p. 3-25

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets

Wüstner, D. & Faergeman, N. J., 2008, In : Cytometry. Part A. 73, 8, p. 727-744 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Color
Adipocytes
Foam Cells
Signal-To-Noise Ratio
Fluorescence Microscopy

Computational analysis of sterol ligand specificity of the Niemann Pick C2 protein

Poongavanam, V., Kongsted, J. & Wüstner, D., 2016, In : Biochemistry. 55, 36, p. 5165-5179

Research output: Contribution to journalJournal articleResearchpeer-review

Computational Characterization of a Cholesterol-Based Molecular Rotor in Lipid Membranes

Reinholdt, P., Wind, S., Wüstner, D. & Kongsted, J., 2019, In : Journal of Physical Chemistry B. 123, 34, p. 7313-7326 14 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
cholesterol
Membrane Lipids
rotors
lipids
13 Downloads (Pure)

Computational Modeling Explains the Multi Sterol Ligand Specificity of the N-Terminal Domain of Niemann-Pick C1-Like 1 Protein

Poongavanam, V., Kongsted, J. & Wüstner, D., 2019, In : ACS Omega. 4, 25, p. 20894-20904 11 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Cholesterol
Sterols
Ligands
Proteins
Molecular mechanics

Computational Modeling of Fluorescence Loss in Photobleaching

Hansen, C. V., Schroll, A. & Wüstner, D., Aug 2015, In : Computing and Visualization in Science. 17, 4, p. 151-166

Research output: Contribution to journalJournal articleResearchpeer-review

Photobleaching
Computational Modeling
Fluorescence
Microscopy
Cell

Dehydroergosterol as an analogue for cholesterol: Why it mimics cholesterol so well?

Pourmosa, M., Rog, T., Mikkeli, R., Vattulaien, I., Solanko, L. M., Wüstner, D., List, N. H., Kongsted, J. & Karttunen, M., 2014, In : Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical. 118, p. 7345

Research output: Contribution to journalJournal articleResearchpeer-review

Design of new fluorescent Cholesterol and Ergosterol analogs: Insights from theory

Nåbo, L. J., List, N. H., Witzke, S., Wüstner, D., Khandelia, H. & Kongsted, J., 2015, In : BBA General Subjects. 1848, 10, part A, p. 2188–2199

Research output: Contribution to journalJournal articleResearchpeer-review

Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells

Wüstner, D., Mondal, M., Huang, A. & Maxfield, F. R., 1. Mar 2004, In : Journal of Lipid Research. 45, 3, p. 427-437

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Sterols
HDL Lipoproteins
Hepatocytes
CD36 Antigens
Apolipoproteins

Direct observation of nuclear receptor dynamics by single molecule tracking and imaging of intrinsically fluorescent ligands

Wüstner, D., 2019.

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells

Wüstner, D., Mondal, M., Tabas, I. & Maxfield, F. R., 1. May 2005, In : Traffic. 6, 5, p. 396-412

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Foam Cells
Macrophages
Sterols
Foams
Observation
152 Downloads (Pure)

Embedding beyond electrostatics: The role of wave function confinement

Nåbo, L. J., Olsen, J. M. H., Holmgaard List, N., Solanko, L. M., Wüstner, D. & Kongsted, J., 14. Sep 2016, In : The Journal of Chemical Physics. 145, 7 p., 104102.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Wave functions
embedding
Electrostatics
wave functions
electrostatics
111 Downloads (Pure)

Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane

Solanko, L. M., Sullivan, D. P., Sere, Y. Y., Szomek, M., Lunding, A., Solanko, K. A., Pizovic, A., Stanchev, L. D., Pomorski, T. G., Menon, A. K. & Wüstner, D., 2018, In : Traffic. 19, 3, p. 198-214

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Ergosterol
Cell membranes
Yeast
Cell Membrane
Sterols

Fluorescent sterols and cholesteryl esters as probes for intracellular cholesterol transport

Solanko, K. A., Modzel, M., Solanko, L. M. & Wüstner, D., 1. Jan 2015, In : Lipid Insights. 8s1, p. 95-114 20 p.

Research output: Contribution to journalReviewResearchpeer-review

Open Access
Cholesterol Esters
Sterols
Cholesterol
Polyenes
Fluorophores

Fluorescent sterols as tools in membrane biophysics and cell biology

Wüstner, D., 2007, In : Chemistry and Physics of Lipids. 146, p. 1-25 25 p.

Research output: Contribution to journalReviewResearch

Following Intracellular Cholesterol Transport by Linear and Non-Linear Optical Microscopy of Intrinsically Fluorescent Sterols

Wustner, D., 2012, In : Current Pharmaceutical Biotechnology. 13, 2, p. 303-318 16 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages

Wüstner, D., 1. Aug 2008, In : Chemistry and Physics of Lipids. 154, 2, p. 129-136 7 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Macrophages
Sterols
Cell membranes
Phase separation
Cholesterol

Glycosylation analysis of a polyreactive human monoclonal IgG antibody derived from a human-mouse heterohybridoma

Leibiger, H., Hansen, A., Schoenherr, G., Seifert, M., Wüstner, D., Stigler, R. & Marx, U., Jun 1995, In : Molecular Immunology. 32, 8, p. 595-602 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Glycosylation
Cell Line
Human Herpesvirus 4
Immunoblotting
Sequence Analysis

Head group-independent interaction of phospholipids with bile salts. A fluorescence and EPR study

Wüstner, D., Herrmann, A. & Müller, P., Mar 2000, In : Journal of Lipid Research. 41, 3, p. 395-404 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Bile Acids and Salts
Paramagnetic resonance
Phospholipids
Fluorescence
Micelles
279 Downloads (Pure)

How cholesterol interacts with proteins and lipids during its intracellular transport

Wüstner, D. & Solanko, K., 2015, In : B B A - Biomembranes. 1848, 9, p. 1908-26

Research output: Contribution to journalReviewResearchpeer-review

Open Access
File
Sterols
Cholesterol
Lipids
Proteins
Cells

Imaging approaches for analysis of cholesterol distribution and dynamics in the plasma membrane

Wüstner, D., Modzel, M., Lund, F. W. & Lomholt, M. A., 2016, In : Chemistry and Physics of Lipids. 199, p. 106-135

Research output: Contribution to journalJournal articleResearchpeer-review

Improved visualization and quantitative analysis of fluorescent membrane sterol in polarized hepatic cells

Wüstner, D., Oct 2005, In : Journal of Microscopy. 220, 1, p. 47-64

Research output: Contribution to journalJournal articleResearchpeer-review

Hepatocytes
Membranes
Recycling
dehydroergosterol
Cholesterol Esters

Intracellular cholesterol transport

Maxfield, F. R. & Wüstner, D., Oct 2002, In : Journal of Clinical Investigation. 110, 7, p. 891-8 8 p.

Research output: Contribution to journalReviewResearchpeer-review

Open Access

Intracellular Cholesterol Transport

Wüstner, D., 2009, Cellular Lipid Metabolism. Ehnholm, C. (ed.). Heidelberg: Springer, p. 157-190 34 p.

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearch

Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells

Hartwig Petersen, N., Færgeman, N. J., Yu, L. & Wüstner, D., 2008, In : Journal of Lipid Research. 49, p. 2023 - 2037

Research output: Contribution to journalJournal articleResearchpeer-review

Sterols
Cell membranes
Recycling
Hepatocellular Carcinoma
Cell Membrane

Live-cell imaging of new polyene sterols for improved analysis of intracellular cholesterol transport

Modzel, M., Solanko, K. A., Szomek, M., Hansen, S. K., Dupont, A., Nåbo, L. J., Kongsted, J. & Wüstner, D., 2018, In : Journal of Microscopy. 271, 1, p. 36-48

Research output: Contribution to journalJournal articleResearchpeer-review

Polyenes
Filipin
Membranes
Fluorescence Microscopy
Microscopy

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses

Benninger, R. K. P., Vanherberghen, B., Young, S., Taner, S. B., Culley, F. J., Schnyder, T., Neil, M. A. A., Wüstner, D., French, P. M. W., Davis, D. M. & Onfelt, B., 1. Jan 2009, In : Biophysical Journal. 96, 2, p. L13-5

Research output: Contribution to journalJournal articleResearchpeer-review

Membranes
Cell Membrane
Optical Imaging
Photons

Mathematical analysis of hepatic high density lipoprotein transport based on quantitative imaging data

Wüstner, D., 25. Feb 2005, In : Journal of Biological Chemistry. 280, 8, p. 6766-6779

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
HDL Lipoproteins
Imaging techniques
Liver
Hepatocytes
Recycling

Mechanism of Cholesterol Sensing in the Niemann Pick Protein (NPC1) using Molecular Dynamics Simulations.

Dubey, V., Bozorg, B., Wüstner, D. & Khandelia, H., 2019.

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure

Solanko, L. M., Wüstner, D., Lund, F. W., Brewer, J. R., Midtiby, H., Bittman, R., Eggeling, C., Honigmann, A. & Dekaris, V., 5. Nov 2013, In : Biophysical Journal. 105, 9, p. 2082-2092 10 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Membranes
Cell membranes
Fluorophores
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

Multicolor bleach-rate imaging enlightens in vivo sterol transport

Wüstner, D. & Sage, D., 2011, In : Communicative & Integrative Biology. 3, 4, p. 370-373 4 p.

Research output: Contribution to journalJournal articleResearchpeer-review

sterols
image analysis
bleaching
endosomes
Caenorhabditis elegans
133 Downloads (Pure)

Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts

Berzina, Z., Solanko, L. M., Mehadi, A. S., Jensen, M. L. V., Lund, F. W., Modzel, M., Szomek, M., Solanko, K. A., Dupont, A., Nielsen, G. K., Heegaard, C. W., Ejsing, C. S. & Wüstner, D., 2018, In : Chemistry and Physics of Lipids. 213, p. 48-61

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
File
Sterols
Fibroblasts
Cell membranes
Cell Membrane
Cholesterol

Photobleaching kinetics and time-integrated emission of fluorescent probes in cellular membranes.

Wüstner, D., Christensen, T., Solanko, L. M. & Sage, D., 29. Jul 2014, In : Molecules. 19, 8, p. 11096-11130 35 p., molecules190811096.

Research output: Contribution to journalJournal articleResearchpeer-review

Open Access
Photobleaching
Bleaching
Fluorescent Dyes
Membranes
Kinetics

Photophysical investigation of two emissive nucleosides exhibiting gigantic stokes shifts

Stendevad, J., Hornum, M., Wüstner, D. & Kongsted, J., 17. Jun 2019, In : Photochemical and Photobiological Sciences. 18, 7, p. 1858-1865 8 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Deoxycytidine
nucleosides
Nucleosides
analogs
shift

Plasma membrane sterol distribution resembles the surface topography of living cells

Wüstner, D., 1. Jan 2007, In : Molecular Biology of the Cell. 18, 1, p. 211-228 18 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells

Wüstner, D., Lund, F. W., Röhrl, C. & Stangl, H., 2016, In : Chemistry and Physics of Lipids. 194, p. 12-28

Research output: Contribution to journalJournal articleResearchpeer-review

Cholesterol
Cells
Fluorescence
Sterols
Cell membranes

Potential of ultraviolet widefield imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes

Wüstner, D., Brewer, J. R., Bagatolli, L. & Sage, D., Jan 2011, In : Microscopy Research and Technique. 74, 1, p. 92-108 17 p.

Research output: Contribution to journalJournal articleResearchpeer-review

Microscopy
Microscopic examination
membranes
microscopy
Membranes

Quantification of polarized traffiking of transferrin and comparison with bulk membrane transport in hepatic cells

Wüstner, D., 2006, In : Biochemical Journal. 400, p. 267-280

Research output: Contribution to journalJournal articleResearchpeer-review

Quantitative analysis of ergosterol transport across the yeast plasma membrane.

Wüstner, D., 2019.

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

Quantitative assessment of sterol traffic in living cells by dual labeling with dehydroergosterol and BODIPY-cholesterol

Wustner, D., Solanko, L., Sokol, O., Garvik, O., Li, Z. G., Bittman, R., Korte, T. & Herrmann, A., 2011, In : Chemistry and Physics of Lipids. 164, 3, p. 221-235 15 p.

Research output: Contribution to journalJournal articleResearchpeer-review