EU (External organisation)

Jørgen Boiden Pedersen (Member)

Activity: MembershipBoard duties in companies, associations, or public organisations

Description

Forskningsleder

n the proposed project we intend to apply a powerful arsenal of spin chemistry 

techniques to study the reactivity, structural and magnetic properties of elusive 

radical intermediates of biologically important molecules. We plan to exploit the 

advantages of the Chemically Induced Dynamic Nuclear Polarization (CIDNP) and 

Magnetic field Affected Reaction Yield (MARY) techniques. Due to their capability 

of sensitively detecting short-lived radical species that are beyond the reach of the 

conventional EPR-spectroscopy, these techniques are suited to provide 

unambiguous determination of HFI constants and g-factors of the transient radicals 

and to allow identifying the elementary steps and mechanisms of the reactions in 

which they are involved. We plan to apply these techniques in particular to 

studying the magnetic resonance properties and reactivity of radicals formed in 

reactions of amino acids and nucleotides, the intramolecular charge transfer in 

peptides, proteins and oligonucleotides, electron transfer reactions between the 

DNA bases and amino acids, and the magnetic field effects on the enzymatic 

phosphorylation reaction. The time-resolved version of CIDNP technique will be 

employed to study the spatial structure and intramolecular mobility of proteins in 

native, denatured and partially folded states. Polarization transfer will be studied in 

detail and utilized to enhance the sensitivity of NMR techniques. A key element of 

the project is its interdisciplinary character comprising biological, chemical, and 

physical approaches. The most important results expected include: 

(a) A detailed analysis of fast reactions involving amino acids and nucleotides, 

including the determination of the reaction mechanisms, the reaction rate 

constants, and characterization of the magnetic resonance properties of the short-lived intermediates formed in the course of these reactions. 

b) The characterization of the mechanisms and the kinetics of the intra-molecular 

charge transfer in peptides, proteins, oligonucleotides, and inter molecular electron 

transfer between amino acids and DNA bases that mimics the DNA repair. 

c) New theoretical methods, suitable for a wide range of spin chemistry 

applications.  

d) New information on the spatial structure and intramolecular mobility of proteins 

in different physical states. 

e) The determination of structural changes in a number of proteins caused by 

ligand and substrate binding, while the efficiency and kinetics of the binding 

process will be measured by utilizing polarization transfer from the protein to the 

ligands. 

f) Determination of the mechanisms of polarization transfer in low magnetic fields, 

exploitation of the remarkable amplitudes of the CIDNP signals to improve the 

notoriously low sensitivity of the NMR of biomolecules. 

g) The identification of the mechanisms responsible for the magnetic field influence 

on enzymatic reactions and the measurement of the magnetic properties of the 

involved radicals. 


Emneord: Kinetics & Reaction Mechanisms, Photochemistry, Proteins, Enzyme Function, Spin Effects
Navn: Forskningsleder

Body type: forskningsnetværk
Period1. Aug 20061. Feb 2009
Held atEU, Belgium
Degree of RecognitionInternational

Keywords

  • Kinetics & Reaction Mechanisms, Photochemistry, Proteins, Enzyme Function, Spin Effects