@article{b4d4afa0a2a311de9743000ea68e967b,
title = "YgdE is the 2'-O-ribose methyltransferase RlmM specific for nucleotide C2498 in bacterial 23S rRNA",
abstract = "The rRNAs of Escherichia coli contain four 2'-O-methylated nucleotides. Similar to other bacterial species and in contrast with Archaea and Eukaryota, the E. coli rRNA modifications are catalysed by specific methyltransferases that find their nucleotide targets without being guided by small complementary RNAs. We show here that the ygdE gene encodes the methyltransferase that catalyses 2'-O-methylation at nucleotide C2498 in the peptidyl transferase loop of E. coli 23S rRNA. Analyses of rRNAs using MALDI mass spectrometry showed that inactivation of the ygdE gene leads to loss of methylation at nucleotide C2498. The loss of ygdE function causes a slight reduction in bacterial fitness. Methylation at C2498 was restored by complementing the knock-out strain with a recombinant copy of ygdE. The recombinant YgdE methyltransferase modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes. Nucleotide C2498 is situated within a highly conserved and heavily modified rRNA sequence, and YgdE's activity is influenced by other modification enzymes that target this region. Phylogenetically, YgdE is placed in the cluster of orthologous groups COG2933 together with S-adenosylmethionine-dependent, Rossmann-fold methyltransferases such as the archaeal and eukaryotic RNA-guided fibrillarins. The ygdE gene has been redesignated rlmM for rRNA large subunit methyltransferase M.",
keywords = "Amino Acid Sequence, Cloning, Molecular, Conserved Sequence, Escherichia coli, Gene Knockout Techniques, Genes, Bacterial, Genetic Complementation Test, Methylation, Methyltransferases, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Bacterial, RNA, Ribosomal, 23S, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Substrate Specificity",
author = "Elzbieta Purta and Michelle O'Connor and Bujnicki, {Janusz M} and Stephen Douthwaite",
year = "2009",
month = jun,
day = "1",
doi = "10.1111/j.1365-2958.2009.06709.x",
language = "English",
volume = "72",
pages = "1147--1158",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "5",
}