Yeast lipids can phase separate into micrometer-scale membrane domains

Christian Klose, Christer S Ejsing, Ana J Garcia-Saez, Hermann-Josef Kaiser, Julio L Sampaio, Michal A Surma, Andrej Shevchenko, Petra Schwille, Kai Simons

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The lipid raft concept proposes that biological membranes have the potential to form functional domains based on a selective interaction between sphingolipids and sterols. These domains seem to be involved in signal transduction and vesicular sorting of proteins and lipids. Although there is biochemical evidence for lipid raft-dependent protein and lipid sorting in the yeast Saccharomyces cerevisiae, direct evidence for an interaction between yeast sphingolipids and the yeast sterol ergosterol, resulting in membrane domain formation, is lacking. Here we show that model membranes formed from yeast total lipid extracts possess an inherent self-organization potential resulting in Ld-Lo phase coexistence at physiologically relevant temperature. Analyses of lipid extracts from mutants defective in sphingolipid metabolism as well as reconstitution of purified yeast lipids in model membranes of defined composition suggest that membrane domain formation depends on specific interactions between yeast sphingolipids and ergosterol. Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast.
OriginalsprogEngelsk
TidsskriftJournal of Biological Chemistry
Vol/bind285
Udgave nummer39
Sider (fra-til)30224-30232
ISSN0021-9258
DOI
StatusUdgivet - 20. jul. 2010

Fingeraftryk

Yeast
Membranes
Lipids
Sphingolipids
Sorting
Ergosterol
Sterols
Biological membranes
Signal transduction
Proteins
Metabolism
Saccharomyces cerevisiae
Chemical analysis

Citer dette

Klose, C., Ejsing, C. S., Garcia-Saez, A. J., Kaiser, H-J., Sampaio, J. L., Surma, M. A., ... Simons, K. (2010). Yeast lipids can phase separate into micrometer-scale membrane domains. Journal of Biological Chemistry, 285(39), 30224-30232. https://doi.org/10.1074/jbc.M110.123554
Klose, Christian ; Ejsing, Christer S ; Garcia-Saez, Ana J ; Kaiser, Hermann-Josef ; Sampaio, Julio L ; Surma, Michal A ; Shevchenko, Andrej ; Schwille, Petra ; Simons, Kai. / Yeast lipids can phase separate into micrometer-scale membrane domains. I: Journal of Biological Chemistry. 2010 ; Bind 285, Nr. 39. s. 30224-30232.
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abstract = "The lipid raft concept proposes that biological membranes have the potential to form functional domains based on a selective interaction between sphingolipids and sterols. These domains seem to be involved in signal transduction and vesicular sorting of proteins and lipids. Although there is biochemical evidence for lipid raft-dependent protein and lipid sorting in the yeast Saccharomyces cerevisiae, direct evidence for an interaction between yeast sphingolipids and the yeast sterol ergosterol, resulting in membrane domain formation, is lacking. Here we show that model membranes formed from yeast total lipid extracts possess an inherent self-organization potential resulting in Ld-Lo phase coexistence at physiologically relevant temperature. Analyses of lipid extracts from mutants defective in sphingolipid metabolism as well as reconstitution of purified yeast lipids in model membranes of defined composition suggest that membrane domain formation depends on specific interactions between yeast sphingolipids and ergosterol. Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast.",
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Klose, C, Ejsing, CS, Garcia-Saez, AJ, Kaiser, H-J, Sampaio, JL, Surma, MA, Shevchenko, A, Schwille, P & Simons, K 2010, 'Yeast lipids can phase separate into micrometer-scale membrane domains', Journal of Biological Chemistry, bind 285, nr. 39, s. 30224-30232. https://doi.org/10.1074/jbc.M110.123554

Yeast lipids can phase separate into micrometer-scale membrane domains. / Klose, Christian; Ejsing, Christer S; Garcia-Saez, Ana J; Kaiser, Hermann-Josef; Sampaio, Julio L; Surma, Michal A; Shevchenko, Andrej; Schwille, Petra; Simons, Kai.

I: Journal of Biological Chemistry, Bind 285, Nr. 39, 20.07.2010, s. 30224-30232.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Yeast lipids can phase separate into micrometer-scale membrane domains

AU - Klose, Christian

AU - Ejsing, Christer S

AU - Garcia-Saez, Ana J

AU - Kaiser, Hermann-Josef

AU - Sampaio, Julio L

AU - Surma, Michal A

AU - Shevchenko, Andrej

AU - Schwille, Petra

AU - Simons, Kai

PY - 2010/7/20

Y1 - 2010/7/20

N2 - The lipid raft concept proposes that biological membranes have the potential to form functional domains based on a selective interaction between sphingolipids and sterols. These domains seem to be involved in signal transduction and vesicular sorting of proteins and lipids. Although there is biochemical evidence for lipid raft-dependent protein and lipid sorting in the yeast Saccharomyces cerevisiae, direct evidence for an interaction between yeast sphingolipids and the yeast sterol ergosterol, resulting in membrane domain formation, is lacking. Here we show that model membranes formed from yeast total lipid extracts possess an inherent self-organization potential resulting in Ld-Lo phase coexistence at physiologically relevant temperature. Analyses of lipid extracts from mutants defective in sphingolipid metabolism as well as reconstitution of purified yeast lipids in model membranes of defined composition suggest that membrane domain formation depends on specific interactions between yeast sphingolipids and ergosterol. Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast.

AB - The lipid raft concept proposes that biological membranes have the potential to form functional domains based on a selective interaction between sphingolipids and sterols. These domains seem to be involved in signal transduction and vesicular sorting of proteins and lipids. Although there is biochemical evidence for lipid raft-dependent protein and lipid sorting in the yeast Saccharomyces cerevisiae, direct evidence for an interaction between yeast sphingolipids and the yeast sterol ergosterol, resulting in membrane domain formation, is lacking. Here we show that model membranes formed from yeast total lipid extracts possess an inherent self-organization potential resulting in Ld-Lo phase coexistence at physiologically relevant temperature. Analyses of lipid extracts from mutants defective in sphingolipid metabolism as well as reconstitution of purified yeast lipids in model membranes of defined composition suggest that membrane domain formation depends on specific interactions between yeast sphingolipids and ergosterol. Taken together, these results provide a mechanistic explanation for lipid raft-dependent lipid and protein sorting in yeast.

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DO - 10.1074/jbc.M110.123554

M3 - Journal article

C2 - 20647309

VL - 285

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

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ER -

Klose C, Ejsing CS, Garcia-Saez AJ, Kaiser H-J, Sampaio JL, Surma MA et al. Yeast lipids can phase separate into micrometer-scale membrane domains. Journal of Biological Chemistry. 2010 jul 20;285(39):30224-30232. https://doi.org/10.1074/jbc.M110.123554