@article{c2b21ce0491011ddb1a1000ea68e967b,
title = "Yeast acyl-CoA-binding protein: acyl-CoA-binding affinity and effect on intracellular acyl-CoA pool size.",
abstract = "Acyl-CoA-binding protein (ACBP) is a 10 kDa protein characterized in vertebrates. We have isolated two ACBP homologues from the yeast Saccharomyces carlsbergensis, named yeast ACBP types 1 and 2. Both proteins contain 86 amino acid residues and are identical except for four conservative substitutions. In comparison with human ACBP, yeast ACBPs exhibit 48% (type 1) and 49% (type 2) conservation of amino acid residues. The amino acid sequence of S. carlsbergensis ACBP type 1 was found to be identical with the one ACBP present in Saccharomyces cerevisiae. A recombinant form of this protein was expressed in Escherichia coli and S. cerevisiae, purified, and its acyl-CoA-binding properties were characterized by isoelectric focusing and microcalorimetric analyses. The yeast ACBP was found to bind acyl-CoA esters with high affinity (Kd 0.55 x 10(-10) M). Overexpression of yeast ACBP in S. cerevisiae resulted in a significant expansion of the intracellular acyl-CoA pool. Finally, Southern-blotting analysis of the two genes encoding ACBP types 1 and 2 in S. carlsbergensis strongly indicated that this species is a hybrid between S. cerevisiae and Saccharomyces monacensis.",
keywords = "Acyl Coenzyme A, Amino Acid Sequence, Base Sequence, Blotting, Southern, Calorimetry, Carrier Proteins, Diazepam Binding Inhibitor, Electron Spin Resonance Spectroscopy, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Genes, Fungal, Genome, Fungal, Humans, Isoelectric Point, Mass Spectrometry, Molecular Sequence Data, Recombinant Proteins, Saccharomyces, Saccharomyces cerevisiae, Sequence Alignment",
author = "J Knudsen and Faergeman, {N J} and H Sk{\o}tt and R Hummel and C B{\o}rsting and Rose, {T M} and Andersen, {Jens S.} and P H{\o}jrup and P Roepstorff and K Kristiansen",
year = "1994",
month = sep,
day = "1",
doi = "10.1042/bj3020479",
language = "English",
volume = "302 ( Pt 2)",
pages = "479--85",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd",
}