XK-related protein 5 (XKR5) is a novel negative regulator of KIT/D816V-mediated transformation

Jianmin Sun, Tine Thingholm, Peter Højrup, Lars Rönnstrand

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

121 Downloads (Pure)


In order to investigate the molecular mechanisms by which the oncogenic mutant KIT/D816V causes transformation of cells, we investigated proteins that selectively bind KIT/D816V, but not wild-type KIT, as potential mediators of transformation. By mass spectrometry several proteins were identified, among them a previously uncharacterized protein denoted XKR5 (XK-related protein 5), which is related to the X Kell blood group proteins. We could demonstrate that interaction between XKR5 and KIT/D816V leads to phosphorylation of XKR5 at Tyr 369, Tyr487, and Tyr 543. Tyrosine phosphorylated XKR5 acts as a negative regulator of KIT signaling, which leads to downregulation of phosphorylation of ERK, AKT, and p38. This led to reduced proliferation and colony forming capacity in semi-solid medium. Taken together, our data demonstrate that XKR5 is a novel type of negative regulator of KIT-mediated transformation.
Udgave nummer6
Antal sider11
StatusUdgivet - 18. jun. 2018


Dyk ned i forskningsemnerne om 'XK-related protein 5 (XKR5) is a novel negative regulator of KIT/D816V-mediated transformation'. Sammen danner de et unikt fingeraftryk.