Vancomycin-resistant Enterococcus spp. validation of susceptibility testing and in vitro activity of vancomycin, linezolid, tigecycline and daptomycin

Mathias Rathe, Lise Kristensen, Svend Ellermann-Eriksen, Marianne Kragh Thomsen, Helga Schumacher

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Vancomycin-resistant enterococci (VRE) have emerged to become a significant nosocomial pathogen. However, detection may be challenging and treatment possibilities are limited. Reports of resistance to linezolide, daptomycin and tigecycline underline the need for reliable susceptibility testing with respect to these compounds. We evaluated the in vitro activity of vancomycin, linezolid, daptomycin and tigecycline against a panel of VRE and vancomycin-susceptible enterococci by broth microdilution (BMD). Etest for determination of minimum inhibitory concentration of these four antibiotics and two disc diffusion assays for detecting VRE and for susceptibility testing against tigecycline and linezolid were evaluated. Before susceptibility testing, all isolates were classified by polymerase chain reaction as vanA or vanB gene positive or vanA/B gene negative. Linezolid, daptomycin and tigecycline had excellent in vitro activity towards all isolates. For daptomycin and tigecycline, the overall agreement between BMD and Etest was suboptimal. For both disc diffusion assays, use of current break points was inadequate to detect vancomycin resistance for isolates carrying the vanB gene. Inspection of the inhibition zone for a diffuse edge, as recommended, accurately predicted presence of the vanB gene.
OriginalsprogEngelsk
BogserieAPMIS Supplementum
Vol/bind118
Udgave nummer1
Sider (fra-til)66-73
Antal sider8
ISSN0903-465X
DOI
StatusUdgivet - 1. jan. 2010

Fingeraftryk

Daptomycin
Vancomycin
Vancomycin Resistance
tigecycline
In Vitro Techniques
Vancomycin-Resistant Enterococci
Polymerase Chain Reaction

Citer dette

Rathe, Mathias ; Kristensen, Lise ; Ellermann-Eriksen, Svend ; Thomsen, Marianne Kragh ; Schumacher, Helga. / Vancomycin-resistant Enterococcus spp. validation of susceptibility testing and in vitro activity of vancomycin, linezolid, tigecycline and daptomycin. I: APMIS Supplementum. 2010 ; Bind 118, Nr. 1. s. 66-73.
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abstract = "Vancomycin-resistant enterococci (VRE) have emerged to become a significant nosocomial pathogen. However, detection may be challenging and treatment possibilities are limited. Reports of resistance to linezolide, daptomycin and tigecycline underline the need for reliable susceptibility testing with respect to these compounds. We evaluated the in vitro activity of vancomycin, linezolid, daptomycin and tigecycline against a panel of VRE and vancomycin-susceptible enterococci by broth microdilution (BMD). Etest for determination of minimum inhibitory concentration of these four antibiotics and two disc diffusion assays for detecting VRE and for susceptibility testing against tigecycline and linezolid were evaluated. Before susceptibility testing, all isolates were classified by polymerase chain reaction as vanA or vanB gene positive or vanA/B gene negative. Linezolid, daptomycin and tigecycline had excellent in vitro activity towards all isolates. For daptomycin and tigecycline, the overall agreement between BMD and Etest was suboptimal. For both disc diffusion assays, use of current break points was inadequate to detect vancomycin resistance for isolates carrying the vanB gene. Inspection of the inhibition zone for a diffuse edge, as recommended, accurately predicted presence of the vanB gene.",
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Vancomycin-resistant Enterococcus spp. validation of susceptibility testing and in vitro activity of vancomycin, linezolid, tigecycline and daptomycin. / Rathe, Mathias; Kristensen, Lise; Ellermann-Eriksen, Svend; Thomsen, Marianne Kragh; Schumacher, Helga.

I: APMIS Supplementum, Bind 118, Nr. 1, 01.01.2010, s. 66-73.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Vancomycin-resistant Enterococcus spp.

T2 - validation of susceptibility testing and in vitro activity of vancomycin, linezolid, tigecycline and daptomycin

AU - Rathe, Mathias

AU - Kristensen, Lise

AU - Ellermann-Eriksen, Svend

AU - Thomsen, Marianne Kragh

AU - Schumacher, Helga

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Vancomycin-resistant enterococci (VRE) have emerged to become a significant nosocomial pathogen. However, detection may be challenging and treatment possibilities are limited. Reports of resistance to linezolide, daptomycin and tigecycline underline the need for reliable susceptibility testing with respect to these compounds. We evaluated the in vitro activity of vancomycin, linezolid, daptomycin and tigecycline against a panel of VRE and vancomycin-susceptible enterococci by broth microdilution (BMD). Etest for determination of minimum inhibitory concentration of these four antibiotics and two disc diffusion assays for detecting VRE and for susceptibility testing against tigecycline and linezolid were evaluated. Before susceptibility testing, all isolates were classified by polymerase chain reaction as vanA or vanB gene positive or vanA/B gene negative. Linezolid, daptomycin and tigecycline had excellent in vitro activity towards all isolates. For daptomycin and tigecycline, the overall agreement between BMD and Etest was suboptimal. For both disc diffusion assays, use of current break points was inadequate to detect vancomycin resistance for isolates carrying the vanB gene. Inspection of the inhibition zone for a diffuse edge, as recommended, accurately predicted presence of the vanB gene.

AB - Vancomycin-resistant enterococci (VRE) have emerged to become a significant nosocomial pathogen. However, detection may be challenging and treatment possibilities are limited. Reports of resistance to linezolide, daptomycin and tigecycline underline the need for reliable susceptibility testing with respect to these compounds. We evaluated the in vitro activity of vancomycin, linezolid, daptomycin and tigecycline against a panel of VRE and vancomycin-susceptible enterococci by broth microdilution (BMD). Etest for determination of minimum inhibitory concentration of these four antibiotics and two disc diffusion assays for detecting VRE and for susceptibility testing against tigecycline and linezolid were evaluated. Before susceptibility testing, all isolates were classified by polymerase chain reaction as vanA or vanB gene positive or vanA/B gene negative. Linezolid, daptomycin and tigecycline had excellent in vitro activity towards all isolates. For daptomycin and tigecycline, the overall agreement between BMD and Etest was suboptimal. For both disc diffusion assays, use of current break points was inadequate to detect vancomycin resistance for isolates carrying the vanB gene. Inspection of the inhibition zone for a diffuse edge, as recommended, accurately predicted presence of the vanB gene.

KW - Acetamides

KW - Animals

KW - Anti-Bacterial Agents

KW - Bacterial Proteins

KW - Carbon-Oxygen Ligases

KW - DNA, Bacterial

KW - Daptomycin

KW - Enterococcus faecalis

KW - Enterococcus faecium

KW - Gram-Positive Bacterial Infections

KW - Humans

KW - Microbial Sensitivity Tests

KW - Minocycline

KW - Oxazolidinones

KW - Polymerase Chain Reaction

KW - Reproducibility of Results

KW - Vancomycin

KW - Vancomycin Resistance

U2 - 10.1111/j.1600-0463.2009.02559.x

DO - 10.1111/j.1600-0463.2009.02559.x

M3 - Journal article

C2 - 20041873

VL - 118

SP - 66

EP - 73

JO - APMIS Supplementum

JF - APMIS Supplementum

SN - 0903-465X

IS - 1

ER -