Validation of peptide and PTM identifications by chemical perturbation proteomics

Jakob Bunkenborg, Lasse Gaarde Falkenby, Lea Mørch Harder, Jens S. Andersen

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Abstrakt

Identification and characterization of proteins via database searching of tandem mass spectrometry data of peptides has become a central technique in proteomics. The massive amount of data generated precludes manual interpretation and validation of the identifications. The current standard procedure is repeating experiments and estimating false discovery rates by searching reverse or garbled protein sequence databases. One danger of this approach is that erroneous assignments can be made quite consistently and reproducibly by the software based on the measurement of a parent mass and the corresponding fragment ions. By derivatizing peptides the parent and fragment masses have to change consistently leading to non-redundant measurements. Here we explore chemical perturbation as a means of improving the confidence level of identifications.
OriginalsprogEngelsk
Publikationsdato3. jun. 2011
StatusUdgivet - 3. jun. 2011
Begivenhed59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics - Denver, CO, USA
Varighed: 5. jun. 20119. jun. 2011
Konferencens nummer: 59

Konference

Konference59th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics
Nummer59
Land/OmrådeUSA
ByDenver, CO
Periode05/06/201109/06/2011

Bibliografisk note

ASMS 2011, Denver

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