Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187

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Resumé

The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.
OriginalsprogEngelsk
TidsskriftBritish Journal of Pharmacology
Vol/bind65
Udgave nummer1
Sider (fra-til)103-9
Antal sider7
ISSN0007-1188
StatusUdgivet - 1979

Fingeraftryk

Histamine Release
Ionophores
Calcimycin
Mast Cells
Oxidative Phosphorylation

Citer dette

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title = "Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187",
abstract = "The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.",
keywords = "Adenosine Triphosphate, Aerobiosis, Anaerobiosis, Animals, Anti-Bacterial Agents, Calcimycin, Calcium, Glucose, Histamine Release, Male, Mast Cells, Oligomycins, Rats, Time Factors, Uncoupling Agents",
author = "Torben Johansen",
year = "1979",
language = "English",
volume = "65",
pages = "103--9",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
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Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187. / Johansen, Torben.

I: British Journal of Pharmacology, Bind 65, Nr. 1, 1979, s. 103-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187

AU - Johansen, Torben

PY - 1979

Y1 - 1979

N2 - The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.

AB - The role of endogenous adenosine triphosphate (ATP) in histamine release from rat mast cells induced by the ionophore A23187 in vitro has been studied. 2 The amount of histamine released by calcium from rat mast cells primed with the ionophore A23187 was dependent on the ATP content of the mast cells. 3 In aerobic experiments a drastic reduction in mast cell ATP content was found during the time when histamine release induced by A23187 takes place. 4 Anaerobic experiments were performed with metabolic inhibitors (antimycin A, oligomycin, and carbonyl cyanide p-trifluorometroxyphenylnydrazone), which are known to block the energy-dependent calcium uptake by isolated mitochondria. The mast cell ATP content was reduced during A23187-induced histamine release under anaerobic conditions in the presence of glucose. This indicates an increased utilization of ATP during the release process. 5 The observations are consistent with the view that energy requiring processes are involved in ionophore-induced histamine release from rat mast cells although part of the ATP reduction in the aerobic experiments may be due to an uncoupling effect of calcium on the oxidative phosphorylation.

KW - Adenosine Triphosphate

KW - Aerobiosis

KW - Anaerobiosis

KW - Animals

KW - Anti-Bacterial Agents

KW - Calcimycin

KW - Calcium

KW - Glucose

KW - Histamine Release

KW - Male

KW - Mast Cells

KW - Oligomycins

KW - Rats

KW - Time Factors

KW - Uncoupling Agents

M3 - Journal article

VL - 65

SP - 103

EP - 109

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 1

ER -