Urinary plasmin activates collecting duct ENaC current in preeclampsia

Kristian B Buhl, Ulla G Friis, Per Svenningsen, Ambika Gulaveerasingam, Per Glud Ovesen, Britta Frederiksen-Møller, Bente Jespersen, Claus Bistrup, Boye L Jensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P
OriginalsprogEngelsk
TidsskriftHypertension
Vol/bind60
Udgave nummer5
Sider (fra-til)1346-51
Antal sider6
ISSN0194-911X
DOI
StatusUdgivet - 2012

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