Urinary plasmin activates collecting duct ENaC current in preeclampsia

Kristian B Buhl, Ulla G Friis, Per Svenningsen, Ambika Gulaveerasingam, Per Glud Ovesen, Britta Frederiksen-Møller, Bente Jespersen, Claus Bistrup, Boye L Jensen

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Resumé

In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P
OriginalsprogEngelsk
TidsskriftHypertension
Vol/bind60
Udgave nummer5
Sider (fra-til)1346-51
Antal sider6
ISSN0194-911X
DOI
StatusUdgivet - 2012

Fingeraftryk

Fibrinolysin
Pre-Eclampsia
Urine
Creatinine
Nephrotic Syndrome
Aldosterone
Pregnant Women
Albumins
uroplasmin
estropipate

Citer dette

Buhl, Kristian B ; Friis, Ulla G ; Svenningsen, Per ; Gulaveerasingam, Ambika ; Ovesen, Per Glud ; Frederiksen-Møller, Britta ; Jespersen, Bente ; Bistrup, Claus ; Jensen, Boye L. / Urinary plasmin activates collecting duct ENaC current in preeclampsia. I: Hypertension. 2012 ; Bind 60, Nr. 5. s. 1346-51.
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abstract = "In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P",
author = "Buhl, {Kristian B} and Friis, {Ulla G} and Per Svenningsen and Ambika Gulaveerasingam and Ovesen, {Per Glud} and Britta Frederiksen-M{\o}ller and Bente Jespersen and Claus Bistrup and Jensen, {Boye L}",
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Urinary plasmin activates collecting duct ENaC current in preeclampsia. / Buhl, Kristian B; Friis, Ulla G; Svenningsen, Per; Gulaveerasingam, Ambika; Ovesen, Per Glud; Frederiksen-Møller, Britta; Jespersen, Bente; Bistrup, Claus; Jensen, Boye L.

I: Hypertension, Bind 60, Nr. 5, 2012, s. 1346-51.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Urinary plasmin activates collecting duct ENaC current in preeclampsia

AU - Buhl, Kristian B

AU - Friis, Ulla G

AU - Svenningsen, Per

AU - Gulaveerasingam, Ambika

AU - Ovesen, Per Glud

AU - Frederiksen-Møller, Britta

AU - Jespersen, Bente

AU - Bistrup, Claus

AU - Jensen, Boye L

PY - 2012

Y1 - 2012

N2 - In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P

AB - In nephrotic syndrome, plasminogen is aberrantly filtered from plasma to the urinary space and activated along the tubular system. In vitro, plasmin increases ENaC current by proteolytic cleavage of the γ-subunit. It was hypothesized that preeclampsia is associated with plasmin-dependent ability of tubular fluid to activate ENaC. Urine was sampled from 16 preeclamptic (PE) patients and 17 normotensive pregnant women (Ctrl). Urine was analyzed for plasmin(ogen), creatinine, albumin, aldosterone, Na(+), K(+), proteolytic activity, and for its effect on inward current in cortical collecting duct cells (M1 cells) by whole-cell patch clamp. In PE, urine plasmin(ogen): creatinine ratio was elevated 40-fold (geometric mean, 160 versus 4 µg/g; P

U2 - 10.1161/HYPERTENSIONAHA.112.198879

DO - 10.1161/HYPERTENSIONAHA.112.198879

M3 - Journal article

VL - 60

SP - 1346

EP - 1351

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 5

ER -