Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma

Sara Martoreli Silveira, Maria Aparecida Custódio Domingues, Ossamu Butugan, Maria Mitzi Brentani, Silvia Regina Rogatto

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

BACKGROUND: To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions.

METHODS: High-resolution comparative genomic hybridization (HR-CGH) was used in laser-captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real-timereverse transcription polymerase chain reaction (qRT-PCR) in 15 cases.

RESULTS: Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM, CDH1, CTNNB1, FGF18, and SUPT16H. A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down-expression in endothelial cells.

CONCLUSIONS: A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB, FGF18, and SUPT16H were identified as potential molecular markers in JNA.

OriginalsprogEngelsk
TidsskriftHead & Neck (Print Edition)
Vol/bind34
Udgave nummer4
Sider (fra-til)485-492
ISSN1043-3074
StatusUdgivet - 2012
Udgivet eksterntJa

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