Transport of the alpha-amino-mono-carboxylic acid L-alanine by the beta-alanine carrier of the rabbit ileum

Vibeke Andersen, B G Munck

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The proposal that the beta-alanine carrier of the rabbit ileum is a high affinity carrier of the neutral amino acids was examined by means of measurements of influx across the brush border membrane of the intact epithelium using L-alanine as a representative of the neutral amino acids. Confirming the proposal, evidence was provided for mutual competitive inhibition between beta-alanine and L-alanine; and it was also demonstrated that a process contributes to the influx of L-alanine, which is characterized by a maximum rate of transport equal to that of beta-alanine and a Kt, which is equal to the Ki of L-alanine against the influx of beta-alanine. In the concentration range 0.01 to 0.125 mM the influx of L-alanine was found to be linearly related to the concentration indicating a significant unstirred layer influence on present and previous estimates of the Kt values for influx of amino acids across the brush-border membrane of intact intestinal epithelia.
OriginalsprogEngelsk
TidsskriftBBA General Subjects
Vol/bind902
Udgave nummer1
Sider (fra-til)145-8
Antal sider4
ISSN0304-4165
StatusUdgivet - 7. aug. 1987

Fingeraftryk

beta-Alanine
Ileum
Alanine
Rabbits
Neutral Amino Acids
Microvilli
Membranes
Intestinal Mucosa
Epithelium
Amino Acids

Citer dette

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title = "Transport of the alpha-amino-mono-carboxylic acid L-alanine by the beta-alanine carrier of the rabbit ileum",
abstract = "The proposal that the beta-alanine carrier of the rabbit ileum is a high affinity carrier of the neutral amino acids was examined by means of measurements of influx across the brush border membrane of the intact epithelium using L-alanine as a representative of the neutral amino acids. Confirming the proposal, evidence was provided for mutual competitive inhibition between beta-alanine and L-alanine; and it was also demonstrated that a process contributes to the influx of L-alanine, which is characterized by a maximum rate of transport equal to that of beta-alanine and a Kt, which is equal to the Ki of L-alanine against the influx of beta-alanine. In the concentration range 0.01 to 0.125 mM the influx of L-alanine was found to be linearly related to the concentration indicating a significant unstirred layer influence on present and previous estimates of the Kt values for influx of amino acids across the brush-border membrane of intact intestinal epithelia.",
keywords = "Alanine, Animals, Binding, Competitive, Biological Transport, Carrier Proteins, Female, Ileum, Kinetics, Microvilli, Rabbits, beta-Alanine",
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Transport of the alpha-amino-mono-carboxylic acid L-alanine by the beta-alanine carrier of the rabbit ileum. / Andersen, Vibeke; Munck, B G.

I: BBA General Subjects, Bind 902, Nr. 1, 07.08.1987, s. 145-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Transport of the alpha-amino-mono-carboxylic acid L-alanine by the beta-alanine carrier of the rabbit ileum

AU - Andersen, Vibeke

AU - Munck, B G

PY - 1987/8/7

Y1 - 1987/8/7

N2 - The proposal that the beta-alanine carrier of the rabbit ileum is a high affinity carrier of the neutral amino acids was examined by means of measurements of influx across the brush border membrane of the intact epithelium using L-alanine as a representative of the neutral amino acids. Confirming the proposal, evidence was provided for mutual competitive inhibition between beta-alanine and L-alanine; and it was also demonstrated that a process contributes to the influx of L-alanine, which is characterized by a maximum rate of transport equal to that of beta-alanine and a Kt, which is equal to the Ki of L-alanine against the influx of beta-alanine. In the concentration range 0.01 to 0.125 mM the influx of L-alanine was found to be linearly related to the concentration indicating a significant unstirred layer influence on present and previous estimates of the Kt values for influx of amino acids across the brush-border membrane of intact intestinal epithelia.

AB - The proposal that the beta-alanine carrier of the rabbit ileum is a high affinity carrier of the neutral amino acids was examined by means of measurements of influx across the brush border membrane of the intact epithelium using L-alanine as a representative of the neutral amino acids. Confirming the proposal, evidence was provided for mutual competitive inhibition between beta-alanine and L-alanine; and it was also demonstrated that a process contributes to the influx of L-alanine, which is characterized by a maximum rate of transport equal to that of beta-alanine and a Kt, which is equal to the Ki of L-alanine against the influx of beta-alanine. In the concentration range 0.01 to 0.125 mM the influx of L-alanine was found to be linearly related to the concentration indicating a significant unstirred layer influence on present and previous estimates of the Kt values for influx of amino acids across the brush-border membrane of intact intestinal epithelia.

KW - Alanine

KW - Animals

KW - Binding, Competitive

KW - Biological Transport

KW - Carrier Proteins

KW - Female

KW - Ileum

KW - Kinetics

KW - Microvilli

KW - Rabbits

KW - beta-Alanine

M3 - Journal article

C2 - 3111534

VL - 902

SP - 145

EP - 148

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1

ER -