Projekter pr. år
Abstract
Primary cilia are microtubule-based sensory organelles whose assembly and function rely on the conserved bidirectional intraflagellar transport (IFT) system, which is powered by anterograde kinesin-2 and retrograde cytoplasmic dynein-2 motors. Nematodes additionally employ a cell-type-specific kinesin-3 motor, KLP-6, which moves within cilia independently of IFT and regulates ciliary content and function. Here, we provide evidence that a KLP-6 homolog, KIF13B, undergoes bursts of bidirectional movement within primary cilia of cultured immortalized human retinal pigment epithelial (hTERT-RPE1) cells. Anterograde and retrograde intraciliary velocities of KIF13B were similar to those of IFT (as assayed using IFT172-eGFP), but intraciliary movement of KIF13B required its own motor domain and appeared to be cell-type specific. Our work provides the first demonstration of motor-driven, intraciliary movement by a vertebrate kinesin other than kinesin-2 motors.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Cell Science |
Vol/bind | 136 |
Udgave nummer | 5 |
ISSN | 0021-9533 |
DOI | |
Status | Udgivet - 1. mar. 2023 |
Bibliografisk note
© 2022. Published by The Company of Biologists Ltd.Fingeraftryk
Dyk ned i forskningsemnerne om 'Transient accumulation and bidirectional movement of KIF13B in primary cilia'. Sammen danner de et unikt fingeraftryk.-
Novo Nordisk fonden - SDU bioimaging Infrastructure
Brewer, J. (Overordnet koordinator)
01/01/2019 → 31/12/2023
Projekter: Projekt › Forskning
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Towards a Thinking X-ray Microscope: Deep Learning to Predict Fluorescence Labels of Cellular Organelles in SXT
Wüstner, D. (PI)
Projekter: Projekt › Private fonde