Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon

G P van Wezel, I M Krab, S Douthwaite, M J Bibb, E Vijgenboom, L Bosch

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1994-Dec
OriginalsprogEngelsk
TidsskriftMicrobiology
Vol/bind140
Udgave nummer12
Sider (fra-til)3357-3365
Antal sider8
ISSN1350-0872
StatusUdgivet - 1. dec. 1994

Fingeraftryk

Streptomyces coelicolor
Transcription Initiation Site
Sigma Factor
Holoenzymes
rRNA Genes

Citer dette

van Wezel, G. P., Krab, I. M., Douthwaite, S., Bibb, M. J., Vijgenboom, E., & Bosch, L. (1994). Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon. Microbiology, 140(12), 3357-3365.
van Wezel, G P ; Krab, I M ; Douthwaite, S ; Bibb, M J ; Vijgenboom, E ; Bosch, L. / Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon. I: Microbiology. 1994 ; Bind 140, Nr. 12. s. 3357-3365.
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title = "Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon",
abstract = "Transcription start sites and processing sites of the Streptomyces coelicolor A3(2) rrnA operon have been investigated by a combination of in vivo and in vitro transcription analyses. The data from these approaches are consistent with the existence of four in vivo transcription sites, corresponding to the promoters P1-P4. The transcription start sites are located at -597, -416, -334 and -254 relative to the start of the 16S rRNA gene. Two putative processing sites were identified, one of which is similar to a sequence reported earlier in S. coelicolor and other eubacteria. The P1 promoter is likely to be recognized by the RNA polymerase holoenzyme containing sigma hrdB, the principal sigma factor in S. coelicolor. P2 also shares homology with the consensus for vegetative promoters, but has a sequence overlapping the consensus -35 region that is also present in the -35 regions of P3 and P4. The -35 sequence common to P2, P3 and P4 is not similar to any other known consensus promoter sequence. In fast-growing mycelium, P2 appears to be the most frequently used promoter. Transcription from all of the rrnA promoters decreased during the transition from exponential to stationary phase, although transcription from P1 and P2 ceased several hours before that from P3 and P4.",
keywords = "Base Sequence, Chromosome Mapping, DNA Primers, DNA, Bacterial, Genes, Bacterial, Molecular Sequence Data, Operon, Promoter Regions, Genetic, RNA Processing, Post-Transcriptional, RNA, Bacterial, RNA, Ribosomal, 16S, Sequence Homology, Nucleic Acid, Single-Strand Specific DNA and RNA Endonucleases, Streptomyces, Transcription, Genetic",
author = "{van Wezel}, {G P} and Krab, {I M} and S Douthwaite and Bibb, {M J} and E Vijgenboom and L Bosch",
year = "1994",
month = "12",
day = "1",
language = "English",
volume = "140",
pages = "3357--3365",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "12",

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van Wezel, GP, Krab, IM, Douthwaite, S, Bibb, MJ, Vijgenboom, E & Bosch, L 1994, 'Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon', Microbiology, bind 140, nr. 12, s. 3357-3365.

Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon. / van Wezel, G P; Krab, I M; Douthwaite, S; Bibb, M J; Vijgenboom, E; Bosch, L.

I: Microbiology, Bind 140, Nr. 12, 01.12.1994, s. 3357-3365.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon

AU - van Wezel, G P

AU - Krab, I M

AU - Douthwaite, S

AU - Bibb, M J

AU - Vijgenboom, E

AU - Bosch, L

PY - 1994/12/1

Y1 - 1994/12/1

N2 - Transcription start sites and processing sites of the Streptomyces coelicolor A3(2) rrnA operon have been investigated by a combination of in vivo and in vitro transcription analyses. The data from these approaches are consistent with the existence of four in vivo transcription sites, corresponding to the promoters P1-P4. The transcription start sites are located at -597, -416, -334 and -254 relative to the start of the 16S rRNA gene. Two putative processing sites were identified, one of which is similar to a sequence reported earlier in S. coelicolor and other eubacteria. The P1 promoter is likely to be recognized by the RNA polymerase holoenzyme containing sigma hrdB, the principal sigma factor in S. coelicolor. P2 also shares homology with the consensus for vegetative promoters, but has a sequence overlapping the consensus -35 region that is also present in the -35 regions of P3 and P4. The -35 sequence common to P2, P3 and P4 is not similar to any other known consensus promoter sequence. In fast-growing mycelium, P2 appears to be the most frequently used promoter. Transcription from all of the rrnA promoters decreased during the transition from exponential to stationary phase, although transcription from P1 and P2 ceased several hours before that from P3 and P4.

AB - Transcription start sites and processing sites of the Streptomyces coelicolor A3(2) rrnA operon have been investigated by a combination of in vivo and in vitro transcription analyses. The data from these approaches are consistent with the existence of four in vivo transcription sites, corresponding to the promoters P1-P4. The transcription start sites are located at -597, -416, -334 and -254 relative to the start of the 16S rRNA gene. Two putative processing sites were identified, one of which is similar to a sequence reported earlier in S. coelicolor and other eubacteria. The P1 promoter is likely to be recognized by the RNA polymerase holoenzyme containing sigma hrdB, the principal sigma factor in S. coelicolor. P2 also shares homology with the consensus for vegetative promoters, but has a sequence overlapping the consensus -35 region that is also present in the -35 regions of P3 and P4. The -35 sequence common to P2, P3 and P4 is not similar to any other known consensus promoter sequence. In fast-growing mycelium, P2 appears to be the most frequently used promoter. Transcription from all of the rrnA promoters decreased during the transition from exponential to stationary phase, although transcription from P1 and P2 ceased several hours before that from P3 and P4.

KW - Base Sequence

KW - Chromosome Mapping

KW - DNA Primers

KW - DNA, Bacterial

KW - Genes, Bacterial

KW - Molecular Sequence Data

KW - Operon

KW - Promoter Regions, Genetic

KW - RNA Processing, Post-Transcriptional

KW - RNA, Bacterial

KW - RNA, Ribosomal, 16S

KW - Sequence Homology, Nucleic Acid

KW - Single-Strand Specific DNA and RNA Endonucleases

KW - Streptomyces

KW - Transcription, Genetic

M3 - Journal article

VL - 140

SP - 3357

EP - 3365

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 12

ER -

van Wezel GP, Krab IM, Douthwaite S, Bibb MJ, Vijgenboom E, Bosch L. Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon. Microbiology. 1994 dec 1;140(12):3357-3365.