Tight Coupling of Metabolic Oscillations and Intracellular Water Dynamics in Saccharomyces cerevisiae

Henrik Seir Thoke, Asger Tobiesen, Jonathan R. Brewer, Per Lyngs Hansen, Roberto Stock, Lars Folke Olsen, Luis Bagatolli

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Resumé

We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.
OriginalsprogEngelsk
TidsskriftPLOS ONE
Vol/bind10
Udgave nummer2
Antal sider16
DOI
StatusUdgivet - 23. feb. 2015

Emneord

  • Fluorescence
  • Fluorescence microscopy
  • Genetic oscillations
  • Circadian oscillations
  • Glycolysis
  • Yeast
  • Fluorescence imaging
  • Relaxation (Physics)

Citer dette

Thoke, Henrik Seir ; Tobiesen, Asger ; Brewer, Jonathan R. ; Hansen, Per Lyngs ; Stock, Roberto ; Olsen, Lars Folke ; Bagatolli, Luis. / Tight Coupling of Metabolic Oscillations and Intracellular Water Dynamics in Saccharomyces cerevisiae. I: PLOS ONE. 2015 ; Bind 10, Nr. 2.
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abstract = "We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.",
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Tight Coupling of Metabolic Oscillations and Intracellular Water Dynamics in Saccharomyces cerevisiae. / Thoke, Henrik Seir; Tobiesen, Asger; Brewer, Jonathan R.; Hansen, Per Lyngs; Stock, Roberto; Olsen, Lars Folke; Bagatolli, Luis.

I: PLOS ONE, Bind 10, Nr. 2, 23.02.2015.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Tight Coupling of Metabolic Oscillations and Intracellular Water Dynamics in Saccharomyces cerevisiae

AU - Thoke, Henrik Seir

AU - Tobiesen, Asger

AU - Brewer, Jonathan R.

AU - Hansen, Per Lyngs

AU - Stock, Roberto

AU - Olsen, Lars Folke

AU - Bagatolli, Luis

PY - 2015/2/23

Y1 - 2015/2/23

N2 - We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.

AB - We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.

KW - Fluorescence

KW - Fluorescence microscopy

KW - Genetic oscillations

KW - Circadian oscillations

KW - Glycolysis

KW - Yeast

KW - Fluorescence imaging

KW - Relaxation (Physics)

U2 - 10.1371/journal.pone.0117308

DO - 10.1371/journal.pone.0117308

M3 - Journal article

VL - 10

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 2

ER -