Thymoglobulin reduces regulatory T cell specific forkhead box P3 (FOXP3) transcripts in kidney transplant recipients

Qais Waleed Saleh, Subagini Nagaraj, Marianne Rasmussen, Martin Tepel

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Abstract

Background
The expression of the forkhead box P3 (FOXP3) protein is essential for the differentiation of T regulatory cells, which are major regulators of immune tolerance and immunologic activity. Determination of FOXP3 transcripts in peripheral blood mononuclear cells may indicate the level of tolerance in kidney transplant recipients. Now, we evaluated the effect of different induction therapies on FOXP3 transcripts on the first postoperative day.
Methods
Blood samples were collected the first day after transplantation from incident adult kidney transplant recipients. Data concerning induction therapies were collected from medical record review. Ribonucleic acid was extracted from peripheral blood mononuclear cells and used in quantitative reverse transcription polymerase chain reaction. Levels of FOXP3 were normalized to the reference gene β-actin, transformed to a logarithmic scale, and correlated to variables using a Bonferroni corrected Spearman’s correlation.
Results
538 patients were included, of which 70 were recipients of ABO-incompatible donor kidneys, 172 ABO-compatible donor kidneys, and 296 deceased donor kidneys.
Compared to recipients with other immunosuppressive induction therapies, kidney transplant recipients receiving thymoglobulin had significantly lower levels of FOXP3 (log scale, median (IQR)): ABO-incompatible, other, -7.8 (-8.5 to -7.2) vs thymoglobuline, -10.7 (-11.3 to -10.1); p<0.001. ABO-compatible, other, -8.0 (-8.5 to -7.5) vs thymoglobuline -10.1 (-11.1 to -9.8); p<0.001. Deceased donors, other, -8.0 (-8.4 to -7.4) vs thymoglobuline -10.8 (-11.5 to -9.7); p<0.001 (Figure 1). Levels of FOXP3 correlated positively with the lymphocyte counts (r=0.4: p<0.01) (Figure 2) and negatively with number of previous transplantations (r=- 0.2; p<0.01) (Figure 3).
Conclusions
Treatment with thymoglobulin significantly reduces FOXP3 transcripts compared to the reference gene in peripheral blood mononuclear cells.
OriginalsprogEngelsk
Publikationsdato2022
StatusUdgivet - 2022
BegivenhedNordiske Nyredage - Odense, 5000
Varighed: 5. maj 20227. maj 2022

Konference

KonferenceNordiske Nyredage
LokationOdense
By5000
Periode05/05/202207/05/2022

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